Gene description for CHUK
Gene name conserved helix-loop-helix ubiquitous kinase
Gene symbol CHUK
Other names/aliases IKBKA
IKK-alpha
IKK1
IKKA
NFKBIKA
TCF16
Species Homo sapiens
 Database cross references - CHUK
ExoCarta ExoCarta_1147
Vesiclepedia VP_1147
Entrez Gene 1147
HGNC 1974
MIM 600664
UniProt O15111  
 CHUK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CHUK
Molecular Function
    protein homodimerization activity GO:0042803 IDA
    scaffold protein binding GO:0097110 IDA
    IkappaB kinase activity GO:0008384 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase activity GO:0004672 IDA
    protein heterodimerization activity GO:0046982 IDA
Biological Process
    inflammatory response GO:0006954 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    striated muscle cell differentiation GO:0051146 IEA
    lactation GO:0007595 IEA
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    response to drug GO:0042493 IEA
    mammary gland alveolus development GO:0060749 IEA
    I-kappaB kinase/NF-kappaB signaling GO:0007249 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    innate immune response GO:0045087 TAS
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    response to hydroperoxide GO:0033194 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    response to toxic substance GO:0009636 IEA
    Rho protein signal transduction GO:0007266 IEA
    response to amino acid GO:0043200 IEA
    anatomical structure morphogenesis GO:0009653 TAS
    skeletal muscle contraction GO:0003009 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    I-kappaB phosphorylation GO:0007252 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    immune response GO:0006955 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    mammary gland epithelial cell proliferation GO:0033598 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    cellular response to tumor necrosis factor GO:0071356 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    response to virus GO:0009615 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    protein phosphorylation GO:0006468 IDA
    morphogenesis of an epithelial sheet GO:0002011 IEA
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    response to lipopolysaccharide GO:0032496 IEA
    osteoclast differentiation GO:0030316 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
Subcellular Localization
    cytoplasmic side of plasma membrane GO:0009898 ISS
    cytoplasm GO:0005737 IDA
    IkappaB kinase complex GO:0008385 TAS
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    CD40 receptor complex GO:0035631 ISS
 Experiment description of studies that identified CHUK in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CHUK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNFAIP3 7128
Invitro Homo sapiens
Invivo Homo sapiens
2 NALP4  
Affinity Capture-Western Homo sapiens
3 IKBKAP 8518
Invivo Homo sapiens
Invitro Homo sapiens
4 MAP3K14  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 TANK 10010
Affinity Capture-Western Homo sapiens
6 TRAF3IP2  
Invivo Homo sapiens
7 TRAF2 7186
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 TP53 7157
Invivo Homo sapiens
9 PPM1B 5495
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 UBE2E3 10477
Two-hybrid Homo sapiens
11 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 NFKBIA  
Biochemical Activity Homo sapiens
13 CDC37 11140
Reconstituted Complex Homo sapiens
14 IKBKG 8517
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
15 CASP8 841
Invivo Homo sapiens
16 IRAK1  
Affinity Capture-Western Homo sapiens
17 MAP3K1 4214
Invivo Homo sapiens
18 MAP3K8  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
19 MAP3K7  
Affinity Capture-Western Homo sapiens
20 PRKDC 5591
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
21 FANCA  
Two-hybrid Homo sapiens
Co-purification Homo sapiens
22 BCL10  
Invivo Homo sapiens
23 MAP3K11 4296
Invivo Homo sapiens
24 AKT2 208
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
25 NFKB1 4790
Affinity Capture-Western Homo sapiens
26 AKT1 207
Invivo Homo sapiens
27 IKBKB 3551
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
28 IRS1 3667
Affinity Capture-Western Homo sapiens
29 CHUK 1147
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
30 TRPC4AP  
Affinity Capture-Western Homo sapiens
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