Gene description for NBR1
Gene name neighbor of BRCA1 gene 1
Gene symbol NBR1
Other names/aliases 1A1-3B
IAI3B
M17S2
MIG19
Species Homo sapiens
 Database cross references - NBR1
ExoCarta ExoCarta_4077
Vesiclepedia VP_4077
Entrez Gene 4077
HGNC 6746
MIM 166945
UniProt Q14596  
 NBR1 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NBR1
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 IDA
    mitogen-activated protein kinase binding GO:0051019 ISS
Biological Process
    macroautophagy GO:0016236 IBA
    macroautophagy GO:0016236 IDA
    regulation of bone mineralization GO:0030500 ISS
    regulation of stress-activated MAPK cascade GO:0032872 ISS
    negative regulation of osteoblast differentiation GO:0045668 ISS
Subcellular Localization
    phagophore assembly site GO:0000407 IBA
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 IDA
    mitochondrial intermembrane space GO:0005758 IEA
    lysosome GO:0005764 IEA
    late endosome GO:0005770 ISS
    autophagosome GO:0005776 ISS
    peroxisomal membrane GO:0005778 TAS
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    nuclear body GO:0016604 IDA
    M band GO:0031430 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified NBR1 in exosomes
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NBR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 CCNO  
Affinity Capture-MS Homo sapiens
3 GABARAPL1 23710
Affinity Capture-Western Homo sapiens
4 MAP1LC3C  
Affinity Capture-Western Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 ATPIF1 93974
Affinity Capture-MS Homo sapiens
7 MTCH1 23787
Proximity Label-MS Homo sapiens
8 SPRED2 200734
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 NRSN1  
Affinity Capture-MS Homo sapiens
10 IGFBP3 3486
Affinity Capture-MS Homo sapiens
11 ITSN2 50618
Two-hybrid Homo sapiens
12 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
13 CALM1 801
Reconstituted Complex Homo sapiens
14 TROVE2 6738
Affinity Capture-MS Homo sapiens
15 SYT4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 STX4 6810
Proximity Label-MS Homo sapiens
17 CORO6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GSK3A 2931
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
19 MAP1LC3B 81631
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 VANGL2  
Affinity Capture-Western Homo sapiens
21 E2F2 1870
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
22 B3GAT1  
Proximity Label-MS Homo sapiens
23 IMPDH2 3615
Proximity Label-MS Homo sapiens
24 PQLC2L  
Affinity Capture-MS Homo sapiens
25 MARCH5  
Proximity Label-MS Homo sapiens
26 MAP3K3 4215
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 EXD2  
Proximity Label-MS Homo sapiens
28 RGS9BP  
Affinity Capture-MS Homo sapiens
29 HAP1  
Affinity Capture-MS Homo sapiens
30 GTSE1 51512
Affinity Capture-MS Homo sapiens
31 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 AMOT  
Proximity Label-MS Homo sapiens
33 GSK3B 2932
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
34 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PXMP2  
Proximity Label-MS Homo sapiens
36 KRT8 3856
Proximity Label-MS Homo sapiens
37 TOLLIP 54472
Affinity Capture-Western Homo sapiens
38 CEP55 55165
Affinity Capture-Western Homo sapiens
39 RB1CC1 9821
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 LGR5 8549
Affinity Capture-MS Homo sapiens
41 NBR1 4077
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 HTT 3064
Affinity Capture-Western Homo sapiens
43 Spred2  
Two-hybrid Mus musculus
44 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
45 USP8 9101
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
46 DIRAS3  
Proximity Label-MS Homo sapiens
47 GABARAP 11337
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
48 TTN 7273
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
49 CIB1 10519
Two-hybrid Homo sapiens
50 Sqstm1  
Affinity Capture-Western Mus musculus
51 DHFRL1  
Proximity Label-MS Homo sapiens
52 LYPD4  
Affinity Capture-MS Homo sapiens
53 MRPL41 64975
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PHLPP1  
Proximity Label-MS Homo sapiens
55 MAP7 9053
Affinity Capture-MS Homo sapiens
56 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 THEM5  
Affinity Capture-MS Homo sapiens
58 SNAPC4  
Affinity Capture-MS Homo sapiens
59 ANXA1 301
Affinity Capture-Western Homo sapiens
60 CEBPG  
Affinity Capture-MS Homo sapiens
61 DUSP16  
Affinity Capture-MS Homo sapiens
62 OCIAD1 54940
Proximity Label-MS Homo sapiens
63 SSH3 54961
Proximity Label-MS Homo sapiens
64 JUN 3725
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 SCGN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 GOLGA1  
Proximity Label-MS Homo sapiens
68 JUNB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 MFN2 9927
Proximity Label-MS Homo sapiens
70 CYP2C9  
Proximity Label-MS Homo sapiens
71 HAX1  
Proximity Label-MS Homo sapiens
72 TMEM74  
Affinity Capture-MS Homo sapiens
73 CHUK 1147
Affinity Capture-Western Homo sapiens
74 EDDM3B  
Affinity Capture-MS Homo sapiens
75 ACBD5 91452
Proximity Label-MS Homo sapiens
76 RNF43  
Proximity Label-MS Homo sapiens
77 FEZ1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
78 TNK2 10188
Co-localization Homo sapiens
79 TP53 7157
Affinity Capture-MS Homo sapiens
80 MAP2K4 6416
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
81 ATXN1L  
Two-hybrid Homo sapiens
82 Map1lc3b  
Affinity Capture-Western Mus musculus
83 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
84 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
85 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
86 VTA1 51534
Two-hybrid Homo sapiens
87 TRIM37  
Proximity Label-MS Homo sapiens
88 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 DNAJB4 11080
Proximity Label-MS Homo sapiens
90 CEP135  
Proximity Label-MS Homo sapiens
91 ZCCHC10  
Affinity Capture-MS Homo sapiens
92 CLTA 1211
Affinity Capture-MS Homo sapiens
93 TMEM31  
Affinity Capture-MS Homo sapiens
94 CAV1 857
Proximity Label-MS Homo sapiens
95 METAP1 23173
Two-hybrid Homo sapiens
96 LY86  
Affinity Capture-MS Homo sapiens
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