Experiment description of studies that identified GABARAPL2 in exosomes

1 Experiment ID 231 ISEV standards ✘ EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ CD63|CD9 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 232 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 233 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for GABARAPL2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ATG3 64422 Affinity Capture-MS Homo sapiens 2 TBC1D5   Two-hybrid Homo sapiens 3 ULK1   Invitro Homo sapiens Two-hybrid Homo sapiens 4 EZR 7430 Affinity Capture-MS Homo sapiens 5 LRPPRC 10128 Affinity Capture-MS Homo sapiens 6 VDAC1 7416 Affinity Capture-MS Homo sapiens 7 NEK9 91754 Affinity Capture-MS Homo sapiens 8 TTBK1   Affinity Capture-MS Homo sapiens 9 BCL2L13 23786 Two-hybrid Homo sapiens 10 SQSTM1 8878 Two-hybrid Homo sapiens 11 HBG1 3047 Affinity Capture-MS Homo sapiens 12 EIF4G1 1981 Affinity Capture-MS Homo sapiens 13 ATG4B 23192 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 14 NDP52   Two-hybrid Homo sapiens 15 UBA5 79876 Two-hybrid Homo sapiens 16 IRGQ1   Affinity Capture-MS Homo sapiens 17 MLX   Two-hybrid Homo sapiens 18 RAP1A 5906 Biochemical Activity Homo sapiens 19 RAP2A 5911 Biochemical Activity Homo sapiens View the network

Pathways in which GABARAPL2 is involved

No pathways found