Gene description for SMO
Gene name smoothened, frizzled class receptor
Gene symbol SMO
Other names/aliases FZD11
Gx
SMOH
Species Homo sapiens
 Database cross references - SMO
ExoCarta ExoCarta_6608
Vesiclepedia VP_6608
Entrez Gene 6608
HGNC 11119
MIM 601500
UniProt Q99835  
 SMO identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SMO
Molecular Function
    G-protein coupled receptor activity GO:0004930 IBA
    drug binding GO:0008144 IDA
    Wnt-activated receptor activity GO:0042813 IBA
    Wnt-protein binding GO:0017147 IBA
    patched binding GO:0005113 IPI
    protein binding GO:0005515 IPI
Biological Process
    multicellular organism growth GO:0035264 IEA
    osteoblast differentiation GO:0001649 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    dorsal/ventral neural tube patterning GO:0021904 IEA
    regulation of heart morphogenesis GO:2000826 ISS
    midgut development GO:0007494 ISS
    renal system development GO:0072001 IEP
    negative regulation of epithelial cell differentiation GO:0030857 IEA
    positive regulation of neuroblast proliferation GO:0002052 IEA
    skeletal muscle fiber development GO:0048741 IEA
    mammary gland epithelial cell differentiation GO:0060644 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of DNA binding GO:0043392 ISS
    G-protein coupled receptor signaling pathway GO:0007186 IEA
    thalamus development GO:0021794 IEA
    cerebellar cortex morphogenesis GO:0021696 IEA
    smoothened signaling pathway involved in ventral spinal cord patterning GO:0021910 IEA
    positive regulation of protein import into nucleus GO:0042307 IEA
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    myoblast migration GO:0051451 IEA
    determination of left/right asymmetry in lateral mesoderm GO:0003140 ISS
    hair follicle morphogenesis GO:0031069 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    in utero embryonic development GO:0001701 IEA
    non-canonical Wnt signaling pathway GO:0035567 IBA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    epithelial-mesenchymal cell signaling GO:0060684 IEA
    somite development GO:0061053 ISS
    heart looping GO:0001947 ISS
    smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation GO:0021938 IEA
    detection of cell density by contact stimulus involved in contact inhibition GO:0060248 IMP
    type B pancreatic cell development GO:0003323 IEA
    protein stabilization GO:0050821 IEA
    canonical Wnt signaling pathway GO:0060070 IBA
    mesenchymal to epithelial transition involved in metanephric renal vesicle formation GO:0072285 ISS
    negative regulation of gene expression GO:0010629 ISS
    ventral midline determination GO:0007371 ISS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    vasculogenesis GO:0001570 IEA
    negative regulation of hair follicle development GO:0051799 IEA
    atrial septum morphogenesis GO:0060413 IEA
    positive regulation of gene expression GO:0010628 ISS
    forebrain morphogenesis GO:0048853 ISS
    pancreas morphogenesis GO:0061113 IEA
    regulation of stem cell maintenance GO:2000036 IEA
    central nervous system neuron differentiation GO:0021953 IEA
    smoothened signaling pathway GO:0007224 IMP
    protein localization to nucleus GO:0034504 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    neural crest cell migration GO:0001755 IEA
    cell fate specification GO:0001708 IEA
    cellular response to cholesterol GO:0071397 ISS
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 ISS
    positive regulation of hh target transcription factor activity GO:0007228 ISS
Subcellular Localization
    plasma membrane GO:0005886 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    integral component of membrane GO:0016021 IBA
    extracellular exosome GO:0070062 IDA
    primary cilium GO:0072372 TAS
    ciliary tip GO:0097542 TAS
    endocytic vesicle membrane GO:0030666 TAS
    Golgi apparatus GO:0005794 IDA
    ciliary membrane GO:0060170 TAS
    caveola GO:0005901 IDA
 Experiment description of studies that identified SMO in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 193
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|CD9
EV Enriched markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTCH1  
Affinity Capture-Western Homo sapiens
2 PTCH2  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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