Gene description for SMO
Gene name smoothened, frizzled class receptor
Gene symbol SMO
Other names/aliases FZD11
Gx
SMOH
Species Homo sapiens
 Database cross references - SMO
ExoCarta ExoCarta_6608
Vesiclepedia VP_6608
Entrez Gene 6608
HGNC 11119
MIM 601500
UniProt Q99835  
 SMO identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SMO
Molecular Function
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IDA
    G protein-coupled receptor activity GO:0004930 IEA
    patched binding GO:0005113 IBA
    patched binding GO:0005113 IPI
    protein binding GO:0005515 IPI
    oxysterol binding GO:0008142 ISS
    protein kinase A catalytic subunit binding GO:0034236 ISS
    protein sequestering activity GO:0140311 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    vasculogenesis GO:0001570 IEA
    osteoblast differentiation GO:0001649 IEA
    in utero embryonic development GO:0001701 IEA
    cell fate specification GO:0001708 IEA
    neural crest cell migration GO:0001755 IEA
    negative regulation of protein phosphorylation GO:0001933 ISS
    heart looping GO:0001947 ISS
    positive regulation of neuroblast proliferation GO:0002052 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    determination of left/right asymmetry in lateral mesoderm GO:0003140 ISS
    type B pancreatic cell development GO:0003323 IEA
    protein import into nucleus GO:0006606 IEA
    apoptotic process GO:0006915 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    smoothened signaling pathway GO:0007224 IBA
    smoothened signaling pathway GO:0007224 IDA
    smoothened signaling pathway GO:0007224 IEP
    smoothened signaling pathway GO:0007224 IMP
    smoothened signaling pathway GO:0007224 ISS
    ventral midline determination GO:0007371 ISS
    pattern specification process GO:0007389 IBA
    neuroblast proliferation GO:0007405 IEA
    central nervous system development GO:0007417 IBA
    midgut development GO:0007494 ISS
    anterior/posterior pattern specification GO:0009952 IEA
    gene expression GO:0010467 IEA
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISS
    negative regulation of gene expression GO:0010629 ISS
    spinal cord dorsal/ventral patterning GO:0021513 IEA
    dentate gyrus development GO:0021542 IEA
    cerebellar cortex morphogenesis GO:0021696 IEA
    thalamus development GO:0021794 IEA
    dorsal/ventral neural tube patterning GO:0021904 IEA
    central nervous system neuron differentiation GO:0021953 IEA
    cerebral cortex development GO:0021987 IEA
    positive regulation of cell migration GO:0030335 IMP
    negative regulation of epithelial cell differentiation GO:0030857 IEA
    hair follicle morphogenesis GO:0031069 IEA
    multicellular organism growth GO:0035264 IEA
    positive regulation of multicellular organism growth GO:0040018 IEA
    positive regulation of protein import into nucleus GO:0042307 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of DNA binding GO:0043392 ISS
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of organ growth GO:0046622 IEA
    astrocyte activation GO:0048143 IEA
    skeletal muscle fiber development GO:0048741 IEA
    smooth muscle tissue development GO:0048745 IEP
    forebrain morphogenesis GO:0048853 ISS
    homeostasis of number of cells within a tissue GO:0048873 IEA
    epithelial cell proliferation GO:0050673 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    protein stabilization GO:0050821 IEA
    myoblast migration GO:0051451 IEA
    negative regulation of hair follicle development GO:0051799 IEA
    contact inhibition GO:0060242 IMP
    atrial septum morphogenesis GO:0060413 IEA
    mammary gland epithelial cell differentiation GO:0060644 IEA
    epithelial-mesenchymal cell signaling GO:0060684 IEA
    somite development GO:0061053 ISS
    pancreas morphogenesis GO:0061113 IEA
    left/right axis specification GO:0070986 IEA
    cellular response to cholesterol GO:0071397 ISS
    dopaminergic neuron differentiation GO:0071542 IEA
    commissural neuron axon guidance GO:0071679 IBA
    mesenchymal to epithelial transition involved in metanephric renal vesicle formation GO:0072285 ISS
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 ISS
    response to inositol GO:1902140 IEA
    regulation of somatic stem cell population maintenance GO:1904672 IEA
    regulation of heart morphogenesis GO:2000826 ISS
Subcellular Localization
    late endosome GO:0005770 IEA
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    Golgi apparatus GO:0005794 IDA
    centriole GO:0005814 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    caveola GO:0005901 IDA
    cilium GO:0005929 IBA
    cilium GO:0005929 IDA
    cilium GO:0005929 ISS
    cilium GO:0005929 TAS
    dendrite GO:0030425 IBA
    endocytic vesicle membrane GO:0030666 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    ciliary membrane GO:0060170 TAS
    extracellular exosome GO:0070062 HDA
    ciliary tip GO:0097542 TAS
    9+0 non-motile cilium GO:0097731 IEA
 Experiment description of studies that identified SMO in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Affinity Capture-Western Homo sapiens
2 HMOX1 3162
Two-hybrid Homo sapiens
3 FAM189B  
Affinity Capture-MS Homo sapiens
4 ARV1  
Two-hybrid Homo sapiens
5 HMGN4  
Two-hybrid Homo sapiens
6 NEDD4 4734
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 CHRNA4  
Affinity Capture-MS Homo sapiens
8 TTYH1  
Affinity Capture-MS Homo sapiens
9 ITGB1BP1 9270
Two-hybrid Homo sapiens
10 OR10H2  
Affinity Capture-MS Homo sapiens
11 JPH3 57338
Two-hybrid Homo sapiens
12 SLC41A3 54946
Two-hybrid Homo sapiens
13 NDFIP1 80762
Two-hybrid Homo sapiens
14 PTCH2  
Affinity Capture-Western Homo sapiens
15 HMOX2 3163
Two-hybrid Homo sapiens
16 S1PR5  
Two-hybrid Homo sapiens
17 EMC10 284361
Two-hybrid Homo sapiens
18 GABARAPL2 11345
Two-hybrid Homo sapiens
19 ATM 472
Negative Genetic Homo sapiens
20 FKBP8 23770
Two-hybrid Homo sapiens
21 GABBR1  
Two-hybrid Homo sapiens
22 TSPAN7  
Two-hybrid Homo sapiens
23 LEPROT 54741
Two-hybrid Homo sapiens
24 NIPA1  
Two-hybrid Homo sapiens
25 WLS 79971
Two-hybrid Homo sapiens
26 SCN4A  
Affinity Capture-MS Homo sapiens
27 C18orf32  
Two-hybrid Homo sapiens
28 PPAPDC3  
Two-hybrid Homo sapiens
29 RAB11A 8766
Proximity Label-MS Homo sapiens
30 TIMMDC1  
Two-hybrid Homo sapiens
31 CHEK1  
Negative Genetic Homo sapiens
32 C4orf3  
Two-hybrid Homo sapiens
33 SSPN 8082
Two-hybrid Homo sapiens
34 BCL6  
Affinity Capture-MS Homo sapiens
35 HERC4 26091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 TMCC2  
Two-hybrid Homo sapiens
37 CLGN 1047
Affinity Capture-MS Homo sapiens
38 PTCH1  
Affinity Capture-Western Homo sapiens
39 C3orf52  
Affinity Capture-MS Homo sapiens
40 RPN2 6185
Two-hybrid Homo sapiens
41 NGEF  
Two-hybrid Homo sapiens
42 KIT 3815
Positive Genetic Homo sapiens
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