Gene description for KIT
Gene name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Gene symbol KIT
Other names/aliases C-Kit
CD117
PBT
SCFR
Species Homo sapiens
 Database cross references - KIT
ExoCarta ExoCarta_3815
Vesiclepedia VP_3815
Entrez Gene 3815
HGNC 6342
MIM 164920
UniProt P10721  
 KIT identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for KIT
Molecular Function
    metal ion binding GO:0046872 IEA
    protease binding GO:0002020 IEA
    protein homodimerization activity GO:0042803 IPI
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IDA
    stem cell factor receptor activity GO:0005020 IEA
    ATP binding GO:0005524 IEA
    receptor signaling protein tyrosine kinase activity GO:0004716 IEA
    cytokine binding GO:0019955 IDA
    protein tyrosine kinase activity GO:0004713 TAS
    protein binding GO:0005515 IPI
Biological Process
    Kit signaling pathway GO:0038109 IDA
    detection of mechanical stimulus involved in sensory perception of sound GO:0050910 ISS
    embryonic hemopoiesis GO:0035162 ISS
    visual learning GO:0008542 IEA
    activation of MAPK activity GO:0000187 IDA
    cytokine-mediated signaling pathway GO:0019221 IDA
    male gonad development GO:0008584 IEP
    cellular response to thyroid hormone stimulus GO:0097067 IEA
    hemopoiesis GO:0030097 TAS
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    actin cytoskeleton reorganization GO:0031532 IDA
    myeloid progenitor cell differentiation GO:0002318 IEA
    positive regulation of tyrosine phosphorylation of Stat5 protein GO:0042523 IMP
    regulation of developmental pigmentation GO:0048070 IEA
    melanocyte differentiation GO:0030318 TAS
    signal transduction by protein phosphorylation GO:0023014 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    lamellipodium assembly GO:0030032 ISS
    positive regulation of tyrosine phosphorylation of Stat1 protein GO:0042511 IMP
    positive regulation of tyrosine phosphorylation of Stat3 protein GO:0042517 IMP
    regulation of cell shape GO:0008360 ISS
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    positive regulation of long-term neuronal synaptic plasticity GO:0048170 IEA
    mast cell degranulation GO:0043303 IMP
    spermatid development GO:0007286 IEA
    epithelial cell proliferation GO:0050673 IEA
    T cell differentiation GO:0030217 ISS
    melanocyte migration GO:0097324 ISS
    positive regulation of Notch signaling pathway GO:0045747 IEA
    positive regulation of cell migration GO:0030335 IEA
    lymphoid progenitor cell differentiation GO:0002320 IEA
    mast cell cytokine production GO:0032762 IDA
    negative regulation of programmed cell death GO:0043069 IEA
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 TAS
    signal transduction GO:0007165 TAS
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IMP
    ovarian follicle development GO:0001541 ISS
    mast cell chemotaxis GO:0002551 IDA
    positive regulation of phospholipase C activity GO:0010863 TAS
    positive regulation of pseudopodium assembly GO:0031274 IEA
    spermatogenesis GO:0007283 TAS
    ectopic germ cell programmed cell death GO:0035234 IEA
    protein autophosphorylation GO:0046777 IDA
    glycosphingolipid metabolic process GO:0006687 IEA
    positive regulation of gene expression GO:0010628 IEA
    digestive tract development GO:0048565 ISS
    somatic stem cell maintenance GO:0035019 IEA
    positive regulation of cell proliferation GO:0008284 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    positive regulation of JAK-STAT cascade GO:0046427 IMP
    immature B cell differentiation GO:0002327 ISS
    dendritic cell cytokine production GO:0002371 ISS
    positive regulation of MAPK cascade GO:0043410 IMP
    mast cell proliferation GO:0070662 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    cell chemotaxis GO:0060326 IDA
    megakaryocyte development GO:0035855 ISS
    innate immune response GO:0045087 TAS
    erythrocyte differentiation GO:0030218 ISS
    regulation of cell proliferation GO:0042127 TAS
    Fc receptor signaling pathway GO:0038093 IDA
    pigmentation GO:0043473 ISS
    somatic stem cell division GO:0048103 IEA
    stem cell maintenance GO:0019827 TAS
    mast cell differentiation GO:0060374 TAS
    stem cell differentiation GO:0048863 ISS
    erythropoietin-mediated signaling pathway GO:0038162 ISS
    inflammatory response GO:0006954 ISS
    germ cell migration GO:0008354 IEA
    melanocyte adhesion GO:0097326 ISS
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
    cytoplasmic side of plasma membrane GO:0009898 IEA
    mast cell granule GO:0042629 IMP
    acrosomal vesicle GO:0001669 IEA
    extracellular space GO:0005615 IDA
    cell-cell junction GO:0005911 IEA
    external side of plasma membrane GO:0009897 IEA
 Experiment description of studies that identified KIT in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 255
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPN11 5781
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
2 KITLG  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
3 INPP5D 3635
Reconstituted Complex Homo sapiens
4 GRB2 2885
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
5 MATK  
Invitro Homo sapiens
Invitro Homo sapiens
6 PTPN6 5777
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
7 GRB7 2886
Reconstituted Complex Homo sapiens
8 CD9 928
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 LCK 3932
Affinity Capture-Western Homo sapiens
10 GRAP  
Invivo Homo sapiens
Invitro Homo sapiens
11 SOCS1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 GRB10 2887
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
13 LYN 4067
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
14 RFC1 5981
Affinity Capture-Western Homo sapiens
15 TEC 7006
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 DOK1 1796
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 STAT1 6772
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
18 CRKL 1399
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
19 YES1 7525
Reconstituted Complex Homo sapiens
20 CD81 975
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 PIK3R2 5296
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
22 PIK3R1 5295
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 CLTC 1213
Affinity Capture-Western Homo sapiens
24 CD63 967
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 PLCG1 5335
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
26 PTPRO 5800
Invivo Homo sapiens
27 MPDZ  
Invitro Homo sapiens
Two-hybrid Homo sapiens
28 JAK2 3717
Affinity Capture-Western Homo sapiens
29 BCR 613
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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