Gene description for ZAP70
Gene name zeta-chain (TCR) associated protein kinase 70kDa
Gene symbol ZAP70
Other names/aliases SRK
STCD
STD
TZK
ZAP-70
Species Homo sapiens
 Database cross references - ZAP70
ExoCarta ExoCarta_7535
Vesiclepedia VP_7535
Entrez Gene 7535
HGNC 12858
MIM 176947
UniProt P43403  
 ZAP70 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for ZAP70
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    protein tyrosine kinase activity GO:0004713 IBA
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 NAS
    protein tyrosine kinase activity GO:0004713 TAS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 NAS
    histone H3Y41 kinase activity GO:0035401 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    adaptive immune response GO:0002250 TAS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 NAS
    immune response GO:0006955 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IMP
    calcium-mediated signaling GO:0019722 IEA
    T cell differentiation GO:0030217 NAS
    intracellular signal transduction GO:0035556 NAS
    T cell activation GO:0042110 TAS
    B cell activation GO:0042113 TAS
    beta selection GO:0043366 IEA
    positive thymic T cell selection GO:0045059 IDA
    negative thymic T cell selection GO:0045060 IEA
    positive regulation of T cell differentiation GO:0045582 IDA
    positive regulation of alpha-beta T cell differentiation GO:0046638 IEA
    positive regulation of alpha-beta T cell proliferation GO:0046641 IEA
    positive regulation of calcium-mediated signaling GO:0050850 IEA
    T cell receptor signaling pathway GO:0050852 IDA
    T cell receptor signaling pathway GO:0050852 TAS
    T cell aggregation GO:0070489 TAS
    T cell migration GO:0072678 TAS
Subcellular Localization
    immunological synapse GO:0001772 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IEA
    T cell receptor complex GO:0042101 IDA
    membrane raft GO:0045121 IDA
 Experiment description of studies that identified ZAP70 in exosomes
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ZAP70
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Affinity Capture-Western Homo sapiens
2 SHC3  
Affinity Capture-Western Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 USP2  
Biochemical Activity Homo sapiens
5 VAV1 7409
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 LAT 27040
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
7 CBLB 868
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 ACP1 52
Affinity Capture-Western Homo sapiens
9 USP12 219333
Proximity Label-MS Homo sapiens
10 ERBB2 2064
Reconstituted Complex Homo sapiens
11 CSK 1445
Reconstituted Complex Homo sapiens
12 PEAR1 375033
Affinity Capture-Western Homo sapiens
13 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 SHC1 6464
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
15 PTPN22  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 SLA  
Affinity Capture-Western Homo sapiens
17 PTPN6 5777
Reconstituted Complex Homo sapiens
18 NR0B2  
Two-hybrid Homo sapiens
19 TYROBP  
Protein-peptide Homo sapiens
20 BTF3 689
Affinity Capture-MS Homo sapiens
21 CD4 920
Affinity Capture-Western Homo sapiens
22 FCRL3  
Reconstituted Complex Homo sapiens
23 ABL1 25
Affinity Capture-Western Homo sapiens
24 RNF41  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
25 LCK 3932
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 PLCG1 5335
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 DBNL 28988
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
28 ERBB4 2066
Reconstituted Complex Homo sapiens
29 SHB 6461
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 EGFR 1956
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Reconstituted Complex Homo sapiens
31 SLA2 84174
Affinity Capture-Western Homo sapiens
32 ERBB3 2065
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
33 GAB2 9846
Affinity Capture-Western Homo sapiens
34 ASB3 51130
Two-hybrid Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 TUBA1A 7846
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
37 CD247 919
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 CBL 867
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
39 HSPA4 3308
Affinity Capture-Western Homo sapiens
40 PAG1 55824
Reconstituted Complex Homo sapiens
41 CD5 921
Affinity Capture-Western Homo sapiens
42 TUBA1B 10376
Affinity Capture-Western Homo sapiens
43 SH3BP2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 UBASH3A 53347
Affinity Capture-Western Homo sapiens
45 PELI1 57162
Reconstituted Complex Homo sapiens
46 TUBB 203068
Affinity Capture-Western Homo sapiens
47 NDUFB8 4714
Two-hybrid Homo sapiens
48 CRK 1398
Reconstituted Complex Homo sapiens
49 LCP2 3937
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
50 ZAP70 7535
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 MET 4233
Reconstituted Complex Homo sapiens
52 SIT1 27240
Affinity Capture-Western Homo sapiens
53 PIK3R1 5295
Affinity Capture-Western Homo sapiens
54 CD38 952
Co-fractionation Homo sapiens
55 FYN 2534
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
56 CD3E 916
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
57 Cd3e  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
58 ANAPC11  
Two-hybrid Homo sapiens
59 KIT 3815
Reconstituted Complex Homo sapiens
60 CBLC  
Protein-peptide Homo sapiens
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