Gene description for GAB2
Gene name GRB2-associated binding protein 2
Gene symbol GAB2
Other names/aliases -
Species Homo sapiens
 Database cross references - GAB2
ExoCarta ExoCarta_9846
Vesiclepedia VP_9846
Entrez Gene 9846
HGNC 14458
MIM 606203
UniProt Q9UQC2  
 GAB2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GAB2
Molecular Function
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IBA
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IDA
    protein binding GO:0005515 IPI
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISS
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 ISS
Biological Process
    signal transduction GO:0007165 IBA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IEA
    positive regulation of cell population proliferation GO:0008284 IDA
    osteoclast differentiation GO:0030316 IMP
    positive regulation of mast cell degranulation GO:0043306 ISS
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane raft GO:0045121 ISS
 Experiment description of studies that identified GAB2 in exosomes
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GAB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
2 GRAP2 9402
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 HDAC4  
Affinity Capture-MS Homo sapiens
4 ETV6  
Affinity Capture-Western Homo sapiens
5 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 KSR1  
Affinity Capture-MS Homo sapiens
7 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CRK 1398
Affinity Capture-MS Homo sapiens
9 ZAP70 7535
Affinity Capture-Western Homo sapiens
10 CDC25B 994
Affinity Capture-MS Homo sapiens
11 SRGAP2 23380
Affinity Capture-MS Homo sapiens
12 CBY1  
Affinity Capture-MS Homo sapiens
13 EIF4E2  
Affinity Capture-MS Homo sapiens
14 CD247 919
Affinity Capture-Western Homo sapiens
15 KIF13B 23303
Affinity Capture-MS Homo sapiens
16 MARK2 2011
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 PLCG2 5336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 ZBTB21  
Affinity Capture-MS Homo sapiens
19 ZNF638 27332
Affinity Capture-MS Homo sapiens
20 RAB3IP  
Affinity Capture-MS Homo sapiens
21 SRC 6714
Affinity Capture-Western Homo sapiens
22 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PLCG1 5335
Reconstituted Complex Homo sapiens
24 YWHAH 7533
Affinity Capture-MS Homo sapiens
25 LRFN1  
Affinity Capture-MS Homo sapiens
26 TRAPPC11 60684
Affinity Capture-MS Homo sapiens
27 NCK1 4690
Reconstituted Complex Homo sapiens
28 YWHAB 7529
Affinity Capture-MS Homo sapiens
29 MAPK1 5594
Biochemical Activity Homo sapiens
30 PTPN6 5777
Affinity Capture-Western Homo sapiens
31 TBC1D14 57533
Affinity Capture-MS Homo sapiens
32 CDK16 5127
Affinity Capture-MS Homo sapiens
33 MAPK3 5595
Biochemical Activity Homo sapiens
34 SET 6418
Affinity Capture-MS Homo sapiens
35 ANKRD34A  
Affinity Capture-MS Homo sapiens
36 USP21  
Affinity Capture-MS Homo sapiens
37 FAM110A  
Affinity Capture-MS Homo sapiens
38 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
39 TESK2  
Affinity Capture-MS Homo sapiens
40 LYN 4067
Affinity Capture-Western Homo sapiens
41 TGM4 7047
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PIK3CB 5291
Affinity Capture-Western Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 CRKL 1399
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
45 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
46 YWHAQ 10971
Affinity Capture-MS Homo sapiens
47 PIK3R1 5295
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
48 FAM110B  
Affinity Capture-MS Homo sapiens
49 KCTD3  
Affinity Capture-MS Homo sapiens
50 LIMA1 51474
Affinity Capture-MS Homo sapiens
51 LAT 27040
Affinity Capture-Western Homo sapiens
52 PTPN11 5781
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
53 ARHGAP32  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
54 DCLK1 9201
Affinity Capture-MS Homo sapiens
55 XPO1 7514
Affinity Capture-MS Homo sapiens
56 GRAP  
Reconstituted Complex Homo sapiens
57 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 NADK 65220
Affinity Capture-MS Homo sapiens
59 SYDE1 85360
Affinity Capture-MS Homo sapiens
60 TRAPPC1 58485
Affinity Capture-MS Homo sapiens
61 ANKRD52 283373
Affinity Capture-MS Homo sapiens
62 TRAPPC4 51399
Affinity Capture-MS Homo sapiens
63 GIGYF1  
Affinity Capture-MS Homo sapiens
64 TRAPPC12 51112
Affinity Capture-MS Homo sapiens
65 MET 4233
Affinity Capture-Western Homo sapiens
66 CGN  
Affinity Capture-MS Homo sapiens
67 TRAPPC5 126003
Affinity Capture-MS Homo sapiens
68 AKT1 207
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
69 ANKRD28 23243
Affinity Capture-MS Homo sapiens
70 TBC1D25  
Affinity Capture-MS Homo sapiens
71 TRAPPC6B 122553
Affinity Capture-MS Homo sapiens
72 DENND4C 55667
Affinity Capture-MS Homo sapiens
73 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
74 FAM53C 51307
Affinity Capture-MS Homo sapiens
75 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
76 USP34 9736
Affinity Capture-MS Homo sapiens
77 RTKN 6242
Affinity Capture-MS Homo sapiens
78 DENND1A 57706
Affinity Capture-MS Homo sapiens
79 PPM1H  
Affinity Capture-MS Homo sapiens
80 TRAPPC8 22878
Affinity Capture-MS Homo sapiens
81 SRSF12  
Affinity Capture-MS Homo sapiens
82 TRAPPC13 80006
Affinity Capture-MS Homo sapiens
83 AGAP1  
Affinity Capture-MS Homo sapiens
84 NF1 4763
Affinity Capture-MS Homo sapiens
85 LRRK1 79705
Affinity Capture-MS Homo sapiens
86 FRS2 10818
Affinity Capture-Western Homo sapiens
87 TRAPPC3 27095
Affinity Capture-MS Homo sapiens
88 PIK3R2 5296
Affinity Capture-MS Homo sapiens
89 MAST3  
Affinity Capture-MS Homo sapiens
90 MAGI1  
Affinity Capture-MS Homo sapiens
91 BCR 613
Affinity Capture-Western Homo sapiens
92 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GAB2 is involved
PathwayEvidenceSource
Axon guidance TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
FGFR1 mutant receptor activation TAS Reactome
FLT3 Signaling IEA Reactome
FLT3 Signaling TAS Reactome
FLT3 signaling in disease TAS Reactome
FLT3 signaling in disease IEA Reactome
IGF1R signaling cascade TAS Reactome
Immune System IEA Reactome
Immune System TAS Reactome
Innate Immune System IEA Reactome
Innate Immune System TAS Reactome
Insulin receptor signalling cascade TAS Reactome
Interleukin receptor SHC signaling TAS Reactome
Interleukin-15 signaling IEA Reactome
Interleukin-2 family signaling TAS Reactome
Interleukin-2 family signaling IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling TAS Reactome
Intracellular signaling by second messengers TAS Reactome
IRS-mediated signalling TAS Reactome
IRS-related events triggered by IGF1R TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Nervous system development TAS Reactome
PI3K Cascade TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
RET signaling TAS Reactome
Role of LAT2/NTAL/LAB on calcium mobilization IEA Reactome
Role of LAT2/NTAL/LAB on calcium mobilization TAS Reactome
Signal Transduction TAS Reactome
Signaling by CSF1 (M-CSF) in myeloid cells IEA Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by CSF3 (G-CSF) TAS Reactome
Signaling by cytosolic FGFR1 fusion mutants TAS Reactome
Signaling by FGFR in disease TAS Reactome
Signaling by FGFR1 in disease TAS Reactome
Signaling by FLT3 fusion proteins IEA Reactome
Signaling by FLT3 fusion proteins TAS Reactome
Signaling by FLT3 ITD and TKD mutants TAS Reactome
Signaling by FLT3 ITD and TKD mutants IEA Reactome
Signaling by Insulin receptor TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by Interleukins IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by SCF-KIT TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) TAS Reactome
STAT5 Activation IEA Reactome
STAT5 activation downstream of FLT3 ITD mutants TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here