Gene description for TRAPPC5
Gene name trafficking protein particle complex 5
Gene symbol TRAPPC5
Other names/aliases TRS31
Species Homo sapiens
 Database cross references - TRAPPC5
ExoCarta ExoCarta_126003
Vesiclepedia VP_126003
Entrez Gene 126003
HGNC 23067
UniProt Q8IUR0  
 TRAPPC5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for TRAPPC5
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 NAS
    vesicle coating GO:0006901 NAS
    COPII vesicle coating GO:0048208 NAS
    vesicle tethering GO:0099022 NAS
Subcellular Localization
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 TAS
    TRAPP complex GO:0030008 IDA
    TRAPPI protein complex GO:1990070 IBA
    TRAPPII protein complex GO:1990071 IBA
    TRAPPII protein complex GO:1990071 NAS
    TRAPPIII protein complex GO:1990072 IBA
    TRAPPIII protein complex GO:1990072 NAS
 Experiment description of studies that identified TRAPPC5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRAPPC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAPPC3L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 TRAPPC2 6399
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 RUFY4  
Affinity Capture-MS Homo sapiens
5 TRAPPC9 83696
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CCNDBP1 23582
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TRAPPC11 60684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 IFNA6  
Affinity Capture-MS Homo sapiens
11 TRAPPC1 58485
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TRAPPC10 7109
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CABS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ATG9A 79065
Proximity Label-MS Homo sapiens
15 ZADH2 284273
Affinity Capture-MS Homo sapiens
16 ACAA1 30
Co-fractionation Homo sapiens
17 STMN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TBC1D14 57533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TRAPPC8 22878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PTCH1  
Affinity Capture-MS Homo sapiens
21 ERGIC3 51614
Affinity Capture-MS Homo sapiens
22 ADI1 55256
Affinity Capture-MS Homo sapiens
23 NPY2R  
Affinity Capture-MS Homo sapiens
24 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
25 KLRD1  
Affinity Capture-MS Homo sapiens
26 TRAPPC6A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SLC18A2  
Affinity Capture-MS Homo sapiens
28 TRAPPC13 80006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 AQP3  
Affinity Capture-MS Homo sapiens
30 PRAMEF17  
Affinity Capture-MS Homo sapiens
31 DDX58 23586
Affinity Capture-RNA Homo sapiens
32 TRAPPC2B  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
33 A2M 2
Two-hybrid Homo sapiens
34 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
35 Trappc8  
Affinity Capture-MS Mus musculus
36 HOXB6 3216
Affinity Capture-MS Homo sapiens
37 S100A6 6277
Affinity Capture-MS Homo sapiens
38 PCDHGB1  
Affinity Capture-MS Homo sapiens
39 RAB43 339122
Affinity Capture-MS Homo sapiens
40 BTBD1  
Affinity Capture-MS Homo sapiens
41 C7orf43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SLC22A4 6583
Affinity Capture-MS Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 FAM69A 388650
Affinity Capture-MS Homo sapiens
45 TRAPPC4 51399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MCM2 4171
Affinity Capture-MS Homo sapiens
47 TRAPPC3 27095
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TRAPPC12 51112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 FAM174A 345757
Affinity Capture-MS Homo sapiens
51 KANK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 KRAS 3845
Negative Genetic Homo sapiens
53 ANP32C 23520
Affinity Capture-MS Homo sapiens
54 UBXN6 80700
Affinity Capture-MS Homo sapiens
55 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 BCL2L14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MTL5  
Affinity Capture-MS Homo sapiens
58 GAB2 9846
Affinity Capture-MS Homo sapiens
59 NANOG  
Affinity Capture-MS Homo sapiens
60 Trappc11  
Affinity Capture-MS Mus musculus
61 HMG20A  
Two-hybrid Homo sapiens
62 RANBP17  
Co-fractionation Homo sapiens
63 SLC2A9  
Affinity Capture-MS Homo sapiens
64 GLB1 2720
Affinity Capture-MS Homo sapiens
65 ITGA8 8516
Affinity Capture-MS Homo sapiens
66 POU5F1  
Affinity Capture-MS Homo sapiens
67 TRAPPC6B 122553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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