Gene description for TRAPPC4
Gene name trafficking protein particle complex 4
Gene symbol TRAPPC4
Other names/aliases CGI-104
HSPC172
PTD009
SBDN
SYNBINDIN
TRS23
Species Homo sapiens
 Database cross references - TRAPPC4
ExoCarta ExoCarta_51399
Vesiclepedia VP_51399
Entrez Gene 51399
HGNC 19943
MIM 610971
UniProt Q9Y296  
 TRAPPC4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for TRAPPC4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 NAS
    vesicle coating GO:0006901 NAS
    autophagy GO:0006914 IMP
    dendrite development GO:0016358 ISS
    COPII vesicle coating GO:0048208 NAS
    vesicle tethering GO:0099022 NAS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum GO:0005783 IEA
    Golgi stack GO:0005795 ISS
    cytosol GO:0005829 TAS
    synaptic vesicle GO:0008021 ISS
    TRAPP complex GO:0030008 IBA
    TRAPP complex GO:0030008 IDA
    TRAPP complex GO:0030008 IMP
    dendrite GO:0030425 ISS
    synapse GO:0045202 ISS
    presynaptic active zone GO:0048786 IEA
    postsynaptic density membrane GO:0098839 IEA
    TRAPPII protein complex GO:1990071 NAS
    TRAPPIII protein complex GO:1990072 NAS
 Experiment description of studies that identified TRAPPC4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRAPPC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SART1 9092
Affinity Capture-MS Homo sapiens
2 TRAPPC3L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 TRAPPC2 6399
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
4 MIS12  
Affinity Capture-MS Homo sapiens
5 ACTR2 10097
Affinity Capture-MS Homo sapiens
6 RUFY4  
Affinity Capture-MS Homo sapiens
7 AASDHPPT 60496
Co-fractionation Homo sapiens
8 PDLIM7 9260
Affinity Capture-MS Homo sapiens
9 TRAPPC9 83696
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
10 Mis12  
Affinity Capture-MS Mus musculus
11 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
12 TRAPPC11 60684
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
13 TRAPPC5 126003
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 Cenpe  
Affinity Capture-MS Mus musculus
15 TRAPPC10 7109
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 MTL5  
Affinity Capture-MS Homo sapiens
17 KIF14 9928
Affinity Capture-MS Homo sapiens
18 CABS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ATG9A 79065
Proximity Label-MS Homo sapiens
20 YWHAB 7529
Affinity Capture-MS Homo sapiens
21 ZADH2 284273
Affinity Capture-MS Homo sapiens
22 STMN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TBC1D14 57533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TRAPPC8 22878
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 PNP 4860
Co-fractionation Homo sapiens
26 KCNQ3  
Affinity Capture-MS Homo sapiens
27 ISCA1  
Affinity Capture-MS Homo sapiens
28 PRSS23 11098
Two-hybrid Homo sapiens
29 ADI1 55256
Affinity Capture-MS Homo sapiens
30 PHPT1 29085
Co-fractionation Homo sapiens
31 MB21D2  
Affinity Capture-MS Homo sapiens
32 ARL8A 127829
Affinity Capture-MS Homo sapiens
33 GAB2 9846
Affinity Capture-MS Homo sapiens
34 CCDC102B  
Affinity Capture-MS Homo sapiens
35 AARSD1 80755
Affinity Capture-MS Homo sapiens
36 CMBL 134147
Affinity Capture-MS Homo sapiens
37 TRAPPC1 58485
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HOXB6 3216
Affinity Capture-MS Homo sapiens
39 RRAS2 22800
Affinity Capture-MS Homo sapiens
40 UGDH 7358
Co-fractionation Homo sapiens
41 ARL6IP6  
Affinity Capture-MS Homo sapiens
42 RAB43 339122
Affinity Capture-MS Homo sapiens
43 RAB30 27314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ARL3 403
Affinity Capture-MS Homo sapiens
45 BTBD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 APP 351
Reconstituted Complex Homo sapiens
47 TRAPPC4 51399
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
48 TRAPPC3 27095
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
49 ARL8B 55207
Affinity Capture-MS Homo sapiens
50 TRAPPC12 51112
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
51 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
52 RAB3IP  
Affinity Capture-MS Homo sapiens
53 GLB1 2720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RAB3IL1 5866
Affinity Capture-MS Homo sapiens
55 TMEM102  
Affinity Capture-MS Homo sapiens
56 KANK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 KRAS 3845
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
58 APBA1  
Affinity Capture-MS Homo sapiens
59 BCL2L14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 OTUB2  
Affinity Capture-MS Homo sapiens
61 CNN2 1265
Affinity Capture-MS Homo sapiens
62 TFIP11  
Two-hybrid Homo sapiens
63 SERPINB1 1992
Co-fractionation Homo sapiens
64 TRAPPC2B  
Affinity Capture-MS Homo sapiens
65 GRAMD1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 GATA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 HUNK  
Affinity Capture-MS Homo sapiens
68 TRAPPC6B 122553
Co-fractionation Homo sapiens
69 INTU  
Affinity Capture-MS Homo sapiens
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