Gene description for CMBL
Gene name carboxymethylenebutenolidase homolog (Pseudomonas)
Gene symbol CMBL
Other names/aliases JS-1
Species Homo sapiens
 Database cross references - CMBL
ExoCarta ExoCarta_134147
Vesiclepedia VP_134147
Entrez Gene 134147
HGNC 25090
MIM 613379
UniProt Q96DG6  
 CMBL identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for CMBL
Molecular Function
    hydrolase activity GO:0016787 TAS
Biological Process
    xenobiotic metabolic process GO:0006805 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CMBL in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CMBL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4A2 1974
Affinity Capture-MS Homo sapiens
2 CRBN  
Affinity Capture-MS Homo sapiens
3 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
4 SNCA 6622
Affinity Capture-MS Homo sapiens
5 Crocc  
Affinity Capture-MS Mus musculus
6 ADH5 128
Co-fractionation Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 TRAPPC11 60684
Affinity Capture-MS Homo sapiens
9 ZNF514  
Affinity Capture-MS Homo sapiens
10 SOD2 6648
Co-fractionation Homo sapiens
11 SMARCA2 6595
Affinity Capture-MS Homo sapiens
12 UBE2H 7328
Affinity Capture-MS Homo sapiens
13 CTCF  
Affinity Capture-MS Homo sapiens
14 CD2BP2 10421
Affinity Capture-MS Homo sapiens
15 TRAPPC8 22878
Affinity Capture-MS Homo sapiens
16 SRSF1 6426
Affinity Capture-MS Homo sapiens
17 PGM1 5236
Co-fractionation Homo sapiens
18 QPCT 25797
Affinity Capture-MS Homo sapiens
19 SMURF1 57154
Affinity Capture-MS Homo sapiens
20 SAP30 8819
Affinity Capture-MS Homo sapiens
21 PPME1 51400
Affinity Capture-MS Homo sapiens
22 TRAPPC13 80006
Affinity Capture-MS Homo sapiens
23 STK38L 23012
Affinity Capture-MS Homo sapiens
24 TRA2B 6434
Affinity Capture-MS Homo sapiens
25 AKR1C3 8644
Co-fractionation Homo sapiens
26 TRAPPC1 58485
Affinity Capture-MS Homo sapiens
27 TRIM24  
Affinity Capture-MS Homo sapiens
28 KIAA1429 25962
Affinity Capture-MS Homo sapiens
29 ACP2 53
Co-fractionation Homo sapiens
30 NQO1 1728
Co-fractionation Homo sapiens
31 CAPG 822
Co-fractionation Homo sapiens
32 RPA3 6119
Proximity Label-MS Homo sapiens
33 ARRB1 408
Affinity Capture-MS Homo sapiens
34 TRAPPC4 51399
Affinity Capture-MS Homo sapiens
35 TRAPPC12 51112
Affinity Capture-MS Homo sapiens
36 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
37 SERBP1 26135
Affinity Capture-MS Homo sapiens
38 Chmp6  
Affinity Capture-MS Mus musculus
39 MDH1 4190
Co-fractionation Homo sapiens
40 SMS 6611
Affinity Capture-MS Homo sapiens
41 DDRGK1 65992
Affinity Capture-MS Homo sapiens
42 CUL2 8453
Affinity Capture-MS Homo sapiens
43 H2AFX 3014
Affinity Capture-MS Homo sapiens
44 KIF20B  
Affinity Capture-MS Homo sapiens
45 TSPYL4 23270
Affinity Capture-MS Homo sapiens
46 NAP1L5  
Affinity Capture-MS Homo sapiens
47 ATIC 471
Affinity Capture-MS Homo sapiens
48 PROSC 11212
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CMBL is involved
PathwayEvidenceSource
Biological oxidations TAS Reactome
Metabolism TAS Reactome
Phase I - Functionalization of compounds TAS Reactome





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