Gene description for TRAPPC8
Gene name trafficking protein particle complex 8
Gene symbol TRAPPC8
Other names/aliases GSG1
HsT2706
KIAA1012
TRS85
Species Homo sapiens
 Database cross references - TRAPPC8
ExoCarta ExoCarta_22878
Vesiclepedia VP_22878
Entrez Gene 22878
HGNC 29169
MIM 614136
UniProt Q9Y2L5  
 TRAPPC8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for TRAPPC8
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 NAS
    Golgi organization GO:0007030 IMP
    collagen biosynthetic process GO:0032964 IMP
    COPII vesicle coating GO:0048208 NAS
    vesicle tethering GO:0099022 NAS
Subcellular Localization
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    TRAPP complex GO:0030008 IDA
    TRAPPIII protein complex GO:1990072 IBA
    TRAPPIII protein complex GO:1990072 NAS
 Experiment description of studies that identified TRAPPC8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRAPPC8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAPPC3L  
Two-hybrid Homo sapiens
2 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
3 MIS12  
Affinity Capture-MS Homo sapiens
4 MYL6 4637
Affinity Capture-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 TRAPPC9 83696
Two-hybrid Homo sapiens
7 TECPR1 25851
Affinity Capture-MS Homo sapiens
8 TRAPPC6B 122553
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
9 EBAG9 9166
Proximity Label-MS Homo sapiens
10 TRAPPC5 126003
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TRAPPC10 7109
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
12 PAXIP1  
Two-hybrid Homo sapiens
13 LAMTOR1 55004
Proximity Label-MS Homo sapiens
14 LAMP3  
Proximity Label-MS Homo sapiens
15 GJA1 2697
Proximity Label-MS Homo sapiens
16 NTRK1 4914
Affinity Capture-MS Homo sapiens
17 ACTR2 10097
Affinity Capture-MS Homo sapiens
18 UNK  
Affinity Capture-RNA Homo sapiens
19 RAB9A 9367
Proximity Label-MS Homo sapiens
20 TBC1D14 57533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TRAPPC8 22878
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 RAB43 339122
Affinity Capture-MS Homo sapiens
23 ACAT2 39
Affinity Capture-MS Homo sapiens
24 YWHAB 7529
Affinity Capture-MS Homo sapiens
25 LAMP2 3920
Proximity Label-MS Homo sapiens
26 TMEM132D  
Two-hybrid Homo sapiens
27 TKT 7086
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TRAPPC13 80006
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
30 GAB2 9846
Affinity Capture-MS Homo sapiens
31 TRAPPC2B  
Affinity Capture-MS Homo sapiens
32 CMBL 134147
Affinity Capture-MS Homo sapiens
33 TRAPPC1 58485
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB4A 5867
Proximity Label-MS Homo sapiens
35 ARL8B 55207
Affinity Capture-MS Homo sapiens
36 ERGIC2 51290
Proximity Label-MS Homo sapiens
37 DNAJC5 80331
Proximity Label-MS Homo sapiens
38 ARL3 403
Affinity Capture-MS Homo sapiens
39 TRAPPC11 60684
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
40 RPA3 6119
Proximity Label-MS Homo sapiens
41 BARD1 580
Two-hybrid Homo sapiens
42 TRAPPC4 51399
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 TRAPPC3 27095
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
44 RAB11A 8766
Proximity Label-MS Homo sapiens
45 TRAPPC12 51112
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
46 TRAPPC2L 51693
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
47 KRAS 3845
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
48 Trappc11  
Affinity Capture-MS Mus musculus
49 OTUB2  
Affinity Capture-MS Homo sapiens
50 KLHL14 57565
Affinity Capture-MS Homo sapiens
51 SSRP1 6749
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CHD4 1108
Affinity Capture-MS Homo sapiens
53 CUL4A 8451
Affinity Capture-MS Homo sapiens
54 STX6 10228
Proximity Label-MS Homo sapiens
55 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 EIF4B 1975
Affinity Capture-MS Homo sapiens
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