Gene description for TBC1D14
Gene name TBC1 domain family, member 14
Gene symbol TBC1D14
Other names/aliases -
Species Homo sapiens
 Database cross references - TBC1D14
ExoCarta ExoCarta_57533
Vesiclepedia VP_57533
Entrez Gene 57533
HGNC 29246
MIM 614855
UniProt Q9P2M4  
 TBC1D14 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TBC1D14
Molecular Function
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    autophagy GO:0006914 IEA
    negative regulation of autophagy GO:0010507 IMP
    recycling endosome to Golgi transport GO:0071955 IMP
    regulation of cilium assembly GO:1902017 IMP
    regulation of autophagosome assembly GO:2000785 IBA
    regulation of autophagosome assembly GO:2000785 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    autophagosome GO:0005776 IBA
    autophagosome GO:0005776 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome GO:0055037 IBA
    recycling endosome GO:0055037 IDA
    recycling endosome GO:0055037 TAS
 Experiment description of studies that identified TBC1D14 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TBC1D14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 LRRC15 131578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 Sumo3  
Affinity Capture-MS Mus musculus
4 TRAPPC9 83696
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SELENBP1 8991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ZNF418  
Affinity Capture-MS Homo sapiens
7 TRAPPC11 60684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TRAPPC5 126003
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TRAPPC1 58485
Affinity Capture-MS Homo sapiens
10 TRAPPC10 7109
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 Osgep  
Affinity Capture-MS Mus musculus
12 NXF1 10482
Two-hybrid Homo sapiens
13 HEPHL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 TRAPPC8 22878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 VILL  
Affinity Capture-MS Homo sapiens
17 UBE3A 7337
Two-hybrid Homo sapiens
18 TRAPPC13 80006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GAB2 9846
Affinity Capture-MS Homo sapiens
20 CENPA  
Affinity Capture-MS Homo sapiens
21 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TRAPPC4 51399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TRAPPC3 27095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TRAPPC12 51112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PML 5371
Affinity Capture-MS Homo sapiens
27 TCHH  
Affinity Capture-MS Homo sapiens
28 TGM4 7047
Affinity Capture-MS Homo sapiens
29 TFEB  
Affinity Capture-MS Homo sapiens
30 TRAPPC6B 122553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TBC1D14 is involved
PathwayEvidenceSource
Membrane Trafficking TAS Reactome
Rab regulation of trafficking TAS Reactome
TBC/RABGAPs TAS Reactome
Vesicle-mediated transport TAS Reactome





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