Gene description for ADRBK1
Gene name adrenergic, beta, receptor kinase 1
Gene symbol ADRBK1
Other names/aliases BARK1
BETA-ARK1
GRK2
Species Homo sapiens
 Database cross references - ADRBK1
ExoCarta ExoCarta_156
Vesiclepedia VP_156
Entrez Gene 156
HGNC 289
MIM 109635
UniProt P25098  
 ADRBK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for ADRBK1
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    protein kinase activity GO:0004672 IMP
    protein kinase activity GO:0004672 ISS
    G protein-coupled receptor kinase activity GO:0004703 IBA
    G protein-coupled receptor kinase activity GO:0004703 IMP
    G protein-coupled receptor kinase activity GO:0004703 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    alpha-2A adrenergic receptor binding GO:0031694 ISS
    Edg-2 lysophosphatidic acid receptor binding GO:0031755 IDA
    beta-adrenergic receptor kinase activity GO:0047696 TAS
Biological Process
    regulation of the force of heart contraction GO:0002026 IBA
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 IBA
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 IMP
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 ISS
    negative regulation of the force of heart contraction by chemical signal GO:0003108 IMP
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    G protein-coupled acetylcholine receptor signaling pathway GO:0007213 ISS
    tachykinin receptor signaling pathway GO:0007217 IDA
    heart development GO:0007507 IEA
    peptidyl-serine phosphorylation GO:0018105 ISS
    viral genome replication GO:0019079 IMP
    receptor internalization GO:0031623 IDA
    positive regulation of catecholamine secretion GO:0033605 ISS
    negative regulation of striated muscle contraction GO:0045988 IMP
    symbiont entry into host cell GO:0046718 IMP
    cardiac muscle contraction GO:0060048 IMP
    negative regulation of relaxation of smooth muscle GO:1901081 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    cilium GO:0005929 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 ISS
    cytoplasmic side of mitochondrial outer membrane GO:0032473 IDA
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified ADRBK1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADRBK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FSHR  
Biochemical Activity Homo sapiens
2 DDX6 1656
Co-fractionation Homo sapiens
3 KBTBD7  
Affinity Capture-MS Homo sapiens
4 SNCA 6622
Protein-peptide Homo sapiens
5 FOXR1  
Affinity Capture-MS Homo sapiens
6 PTPRF 5792
Co-fractionation Homo sapiens
7 Rfx5  
Affinity Capture-MS Mus musculus
8 CSNK2B 1460
Two-hybrid Homo sapiens
9 AGTR1  
Affinity Capture-Western Homo sapiens
10 PAPOLA 10914
Co-fractionation Homo sapiens
11 Rapgef3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 GPR17 2840
Affinity Capture-Luminescence Homo sapiens
13 Tnpo1 238799
Affinity Capture-MS Mus musculus
14 WDR1 9948
Co-fractionation Homo sapiens
15 PDCL 5082
Biochemical Activity Homo sapiens
16 GIT2 9815
Affinity Capture-Western Homo sapiens
17 TBC1D14 57533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FERMT2 10979
Co-fractionation Homo sapiens
19 ADRB2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
20 MEX3A  
Affinity Capture-RNA Homo sapiens
21 PYGL 5836
Co-fractionation Homo sapiens
22 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
23 DNAAF5 54919
Co-fractionation Homo sapiens
24 GNAQ 2776
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 SQSTM1 8878
Proximity Label-MS Homo sapiens
26 CALCOCO1 57658
Affinity Capture-MS Homo sapiens
27 ADRBK1 156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MALT1 10892
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 EDNRB 1910
Affinity Capture-Western Homo sapiens
30 PDE6G  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
31 GRM1  
Affinity Capture-Western Homo sapiens
32 PDGFRB 5159
Affinity Capture-Western Homo sapiens
33 PEBP1 5037
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 PRKCB 5579
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 CDC42BPA 8476
Affinity Capture-MS Homo sapiens
36 HDAC6 10013
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
37 ADSL 158
Co-fractionation Homo sapiens
38 ALDH16A1 126133
Co-fractionation Homo sapiens
39 KCNIP3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
40 MED20 9477
Co-fractionation Homo sapiens
41 PPP1R16B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ADRBK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ACACA 31
Affinity Capture-MS Homo sapiens
44 RASGRF1  
Two-hybrid Homo sapiens
45 SLAIN2  
Affinity Capture-MS Homo sapiens
46 MOV10 4343
Affinity Capture-RNA Homo sapiens
47 GIT1 28964
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 PDC  
Biochemical Activity Homo sapiens
49 CCR4 1233
Affinity Capture-Western Homo sapiens
50 PPWD1 23398
Co-fractionation Homo sapiens
51 ARRB1 408
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
52 Nedd4 25489
Biochemical Activity Rattus norvegicus
53 RHO  
Reconstituted Complex Homo sapiens
54 SMO 6608
Affinity Capture-Western Homo sapiens
55 Kif13b  
Affinity Capture-MS Mus musculus
56 GNB1 2782
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
57 PRPF4 9128
Affinity Capture-MS Homo sapiens
58 TRAF2 7186
Affinity Capture-Western Homo sapiens
59 EPHA2 1969
Two-hybrid Homo sapiens
60 BICD2 23299
Proximity Label-MS Homo sapiens
61 BICD1 636
Proximity Label-MS Homo sapiens
62 FBXO25  
Biochemical Activity Homo sapiens
63 SRC 6714
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
64 NARS2  
Co-fractionation Homo sapiens
65 RXRB 6257
Affinity Capture-MS Homo sapiens
66 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
67 CTR9 9646
Affinity Capture-MS Homo sapiens
68 DDX58 23586
Affinity Capture-RNA Homo sapiens
69 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
70 CANX 821
Cross-Linking-MS (XL-MS) Homo sapiens
71 CAV1 857
Reconstituted Complex Homo sapiens
72 TIMP2 7077
Co-fractionation Homo sapiens
73 SCFD2 152579
Affinity Capture-MS Homo sapiens
74 MDM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
75 GNA15 2769
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
76 Malt1  
Affinity Capture-MS Rattus norvegicus
77 PROSC 11212
Co-fractionation Homo sapiens
78 GNB2 2783
Biochemical Activity Homo sapiens
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