Gene description for ARRB1
Gene name arrestin, beta 1
Gene symbol ARRB1
Other names/aliases ARB1
ARR1
Species Homo sapiens
 Database cross references - ARRB1
ExoCarta ExoCarta_408
Vesiclepedia VP_408
Entrez Gene 408
HGNC 711
MIM 107940
UniProt P49407  
 ARRB1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ARRB1
Molecular Function
    insulin-like growth factor receptor binding GO:0005159 IPI
    angiotensin receptor binding GO:0031701 IPI
    enzyme inhibitor activity GO:0004857 TAS
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IEA
    transcription factor binding GO:0008134 IPI
    GTPase activator activity GO:0005096 IMP
    histone acetyltransferase activity GO:0004402 IDA
    protein binding GO:0005515 IPI
    mitogen-activated protein kinase kinase binding GO:0031434 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    transcription regulatory region DNA binding GO:0044212 IMP
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    positive regulation of GTPase activity GO:0043547 IMP
    protein ubiquitination GO:0016567 IMP
    Notch signaling pathway GO:0007219 TAS
    negative regulation of protein ubiquitination GO:0031397 IDA
    membrane organization GO:0061024 TAS
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    platelet activation GO:0030168 TAS
    negative regulation of interleukin-6 production GO:0032715 IDA
    G-protein coupled receptor internalization GO:0002031 IMP
    positive regulation of protein binding GO:0032092 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    positive regulation of receptor internalization GO:0002092 IMP
    protein transport GO:0015031 IEA
    positive regulation of histone acetylation GO:0035066 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    positive regulation of Rho protein signal transduction GO:0035025 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    phototransduction GO:0007602 IEA
    positive regulation of histone H4 acetylation GO:0090240 IMP
    negative regulation of interleukin-8 production GO:0032717 IDA
    stress fiber assembly GO:0043149 IMP
    transcription from RNA polymerase II promoter GO:0006366 IMP
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IEA
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IEA
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    blood coagulation GO:0007596 TAS
Subcellular Localization
    coated pit GO:0005905 IEA
    cytosol GO:0005829 TAS
    cytoplasmic vesicle membrane GO:0030659 TAS
    cytoplasm GO:0005737 IDA
    Golgi membrane GO:0000139 TAS
    pseudopodium GO:0031143 IEA
    cytoplasmic vesicle GO:0031410 IDA
    lysosomal membrane GO:0005765 TAS
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 TAS
    nucleus GO:0005634 IDA
    chromatin GO:0000785 IDA
    heterotrimeric G-protein complex GO:0005834 TAS
 Experiment description of studies that identified ARRB1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ARRB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 NSF 4905
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 PTHR1  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 ARRB2 409
Affinity Capture-MS Homo sapiens
5 GNMT 27232
Two-hybrid Homo sapiens
6 KPNA3 3839
Affinity Capture-MS Homo sapiens
7 CSK 1445
Affinity Capture-Western Homo sapiens
8 CCL14  
Affinity Capture-MS Homo sapiens
9 DVL2 1856
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 RALGDS  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 PES1 23481
Affinity Capture-MS Homo sapiens
12 KHK 3795
Affinity Capture-MS Homo sapiens
13 CHD1 1105
Affinity Capture-MS Homo sapiens
14 OPRD1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 NFKBIA  
Affinity Capture-MS Homo sapiens
16 BOP1 23246
Affinity Capture-MS Homo sapiens
17 ARR3 407
Affinity Capture-MS Homo sapiens
18 PDE4D  
Affinity Capture-MS Homo sapiens
19 ADRB1  
Reconstituted Complex Homo sapiens
20 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
21 CYTH2 9266
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
22 ARF6 382
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 IL8RB  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
24 RPL7L1 285855
Affinity Capture-MS Homo sapiens
25 DVL1 1855
Affinity Capture-Western Homo sapiens
26 CMBL 134147
Affinity Capture-MS Homo sapiens
27 MAPK10 5602
Affinity Capture-Western Homo sapiens
28 RTF1 23168
Affinity Capture-MS Homo sapiens
29 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
30 ZRANB2 9406
Affinity Capture-MS Homo sapiens
31 JAK1 3716
Affinity Capture-MS Homo sapiens
32 NOLC1 9221
Affinity Capture-MS Homo sapiens
33 CCR5 1234
Reconstituted Complex Homo sapiens
34 TCOF1 6949
Affinity Capture-MS Homo sapiens
35 CCR2  
Invivo Homo sapiens
36 AP3B1 8546
Affinity Capture-MS Homo sapiens
37 PTHLH  
Affinity Capture-MS Homo sapiens
38 TRHR  
Invitro Homo sapiens
39 DDX27 55661
Affinity Capture-MS Homo sapiens
40 ZBTB43  
Two-hybrid Homo sapiens
View the network image/svg+xml



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