Gene description for KHK
Gene name ketohexokinase (fructokinase)
Gene symbol KHK
Other names/aliases -
Species Homo sapiens
 Database cross references - KHK
ExoCarta ExoCarta_3795
Vesiclepedia VP_3795
Entrez Gene 3795
HGNC 6315
MIM 614058
UniProt P50053  
 KHK identified in sEVs derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for KHK
Molecular Function
    ketohexokinase activity GO:0004454 IBA
    ketohexokinase activity GO:0004454 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    identical protein binding GO:0042802 IDA
    protein homodimerization activity GO:0042803 IDA
    fructose binding GO:0070061 IDA
Biological Process
    fructose metabolic process GO:0006000 IBA
    fructose metabolic process GO:0006000 IDA
    fructose metabolic process GO:0006000 IEA
    response to sucrose GO:0009744 IBA
    response to glucose GO:0009749 IBA
    response to fructose GO:0009750 IBA
    response to zinc ion GO:0010043 IBA
    response to insulin GO:0032868 IBA
    carbohydrate phosphorylation GO:0046835 IEA
    regulation of glycogen metabolic process GO:0070873 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KHK in sEVs
1
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for KHK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP2S1 1175
Affinity Capture-MS Homo sapiens
2 DNAJB14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LYZ 4069
Affinity Capture-MS Homo sapiens
4 CUEDC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 UGP2 7360
Co-fractionation Homo sapiens
6 SNRPB 6628
Affinity Capture-MS Homo sapiens
7 LHX2  
Two-hybrid Homo sapiens
8 GLB1 2720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CST8  
Affinity Capture-MS Homo sapiens
10 SULT1C4  
Co-fractionation Homo sapiens
11 RANBP3 8498
Affinity Capture-MS Homo sapiens
12 AGL 178
Co-fractionation Homo sapiens
13 CD2BP2 10421
Affinity Capture-MS Homo sapiens
14 RNPS1 10921
Affinity Capture-MS Homo sapiens
15 ARRB1 408
Affinity Capture-MS Homo sapiens
16 NPEPL1 79716
Co-fractionation Homo sapiens
17 CDK5RAP3 80279
Affinity Capture-MS Homo sapiens
18 COPS5 10987
Affinity Capture-MS Homo sapiens
19 LCN1 3933
Affinity Capture-MS Homo sapiens
20 SPG21 51324
Affinity Capture-MS Homo sapiens
21 DEDD  
Affinity Capture-MS Homo sapiens
22 LHX9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here