Gene description for NPEPL1
Gene name aminopeptidase-like 1
Gene symbol NPEPL1
Other names/aliases bA261P9.2
Species Homo sapiens
 Database cross references - NPEPL1
ExoCarta ExoCarta_79716
Vesiclepedia VP_79716
Entrez Gene 79716
HGNC 16244
UniProt Q8NDH3  
 NPEPL1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NPEPL1
Molecular Function
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IBA
    manganese ion binding GO:0030145 IEA
    metalloaminopeptidase activity GO:0070006 IEA
Biological Process
    proteolysis GO:0006508 IBA
Subcellular Localization
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified NPEPL1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NPEPL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TFCP2 7024
Two-hybrid Homo sapiens
2 CSTF1 1477
Co-fractionation Homo sapiens
3 NIF3L1 60491
Co-fractionation Homo sapiens
4 GDA 9615
Co-fractionation Homo sapiens
5 MSH2 4436
Co-fractionation Homo sapiens
6 TIPRL 261726
Co-fractionation Homo sapiens
7 XPO1 7514
Co-fractionation Homo sapiens
8 TPT1 7178
Co-fractionation Homo sapiens
9 PFKP 5214
Co-fractionation Homo sapiens
10 ALDH8A1 64577
Co-fractionation Homo sapiens
11 MDFI  
Two-hybrid Homo sapiens
12 KHK 3795
Co-fractionation Homo sapiens
13 CAP2 10486
Co-fractionation Homo sapiens
14 ATRIP  
Co-fractionation Homo sapiens
15 NDRG3 57446
Co-fractionation Homo sapiens
16 PPAT 5471
Co-fractionation Homo sapiens
17 ARHGDIA 396
Co-fractionation Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 RB1 5925
Co-fractionation Homo sapiens
20 MAPT  
Co-fractionation Homo sapiens
21 STAT1 6772
Co-fractionation Homo sapiens
22 MCMBP 79892
Co-fractionation Homo sapiens
23 SUMO2 6613
Co-fractionation Homo sapiens
24 PRRC1 133619
Co-fractionation Homo sapiens
25 HMBS 3145
Co-fractionation Homo sapiens
26 PLCG1 5335
Co-fractionation Homo sapiens
27 COMMD4 54939
Co-fractionation Homo sapiens
28 CCDC25 55246
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NPEPL1 is involved
No pathways found





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