Gene description for PPAT
Gene name phosphoribosyl pyrophosphate amidotransferase
Gene symbol PPAT
Other names/aliases ATASE
GPAT
PRAT
Species Homo sapiens
 Database cross references - PPAT
ExoCarta ExoCarta_5471
Entrez Gene 5471
HGNC 9238
MIM 172450
UniProt Q06203  
 PPAT identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PPAT
Molecular Function
    metal ion binding GO:0046872 IEA
    amidophosphoribosyltransferase activity GO:0004044 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
Biological Process
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    organ regeneration GO:0031100 IEA
    purine nucleotide biosynthetic process GO:0006164 TAS
    protein homotetramerization GO:0051289 IEA
    purine nucleobase metabolic process GO:0006144 TAS
    maternal process involved in female pregnancy GO:0060135 IEA
    cellular response to drug GO:0035690 IEA
    kidney development GO:0001822 IEA
    small molecule metabolic process GO:0044281 TAS
    G1/S transition of mitotic cell cycle GO:0000082 IEA
    'de novo' IMP biosynthetic process GO:0006189 IEA
    glutamine catabolic process GO:0006543 IEA
    purine ribonucleoside monophosphate biosynthetic process GO:0009168 TAS
    nucleoside metabolic process GO:0009116 IEA
    purine nucleobase biosynthetic process GO:0009113 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    lactation GO:0007595 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PPAT in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPAT
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PPAT is involved
PathwayEvidenceSource
Purine ribonucleoside monophosphate biosynthesis TAS Reactome





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