Gene description for SNX2
Gene name sorting nexin 2
Gene symbol SNX2
Other names/aliases TRG-9
Species Homo sapiens
 Database cross references - SNX2
ExoCarta ExoCarta_6643
Vesiclepedia VP_6643
Entrez Gene 6643
HGNC 11173
MIM 605929
UniProt O60749  
 SNX2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for SNX2
Molecular Function
    epidermal growth factor receptor binding GO:0005154 IDA
    insulin receptor binding GO:0005158 IDA
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IBA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    cadherin binding GO:0045296 HDA
    protein heterodimerization activity GO:0046982 IPI
    transferrin receptor binding GO:1990459 IDA
    leptin receptor binding GO:1990460 IDA
Biological Process
    intracellular protein transport GO:0006886 IEA
    early endosome to Golgi transport GO:0034498 IBA
    retrograde transport, endosome to Golgi GO:0042147 IMP
    retrograde transport, endosome to Golgi GO:0042147 NAS
    lamellipodium morphogenesis GO:0072673 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IDA
    endosome GO:0005768 IBA
    endosome GO:0005768 IDA
    cytosol GO:0005829 IEA
    endosome membrane GO:0010008 IBA
    endosome membrane GO:0010008 IDA
    membrane GO:0016020 IDA
    lamellipodium GO:0030027 IEA
    retromer complex GO:0030904 IDA
    retromer, tubulation complex GO:0030905 IPI
    retromer, tubulation complex GO:0030905 NAS
    early endosome membrane GO:0031901 IEA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified SNX2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SNX2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNX32  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
3 NCBP1 4686
Co-fractionation Homo sapiens
4 MAN2B1 4125
Co-fractionation Homo sapiens
5 MARCKS 4082
Proximity Label-MS Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 PAWR 5074
Co-fractionation Homo sapiens
9 KCTD10 83892
Affinity Capture-MS Homo sapiens
10 ATP6V1C1 528
Co-fractionation Homo sapiens
11 NFYC 4802
Two-hybrid Homo sapiens
12 C9orf78 51759
Affinity Capture-MS Homo sapiens
13 NTNG1  
Affinity Capture-MS Homo sapiens
14 LAMP2 3920
Proximity Label-MS Homo sapiens
15 ARL6IP1 23204
Two-hybrid Homo sapiens
16 PPAT 5471
Co-fractionation Homo sapiens
17 SNX6 58533
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
18 RAPH1 65059
Co-fractionation Homo sapiens
19 VPS29 51699
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
20 DNAJB6 10049
Affinity Capture-MS Homo sapiens
21 VPS26A 9559
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
22 LAMC1 3915
Two-hybrid Homo sapiens
23 TCTEX1D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GSPT2 23708
Co-fractionation Homo sapiens
25 RDX 5962
Co-fractionation Homo sapiens
26 PDXK 8566
Co-fractionation Homo sapiens
27 IMPDH2 3615
Co-fractionation Homo sapiens
28 PCID2 55795
Co-fractionation Homo sapiens
29 GNAO1 2775
Affinity Capture-MS Homo sapiens
30 HIRIP3  
Co-fractionation Homo sapiens
31 HGS 9146
Co-fractionation Homo sapiens
32 FAM221A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 IGH 3492
Affinity Capture-MS Homo sapiens
34 TREML2 79865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ERC1 23085
Co-fractionation Homo sapiens
36 TRIM56 81844
Co-fractionation Homo sapiens
37 CCDC53 51019
Affinity Capture-MS Homo sapiens
38 TDG 6996
Co-fractionation Homo sapiens
39 SNX3 8724
Co-fractionation Homo sapiens
40 GSPT1 2935
Co-fractionation Homo sapiens
41 TBC1D15 64786
Co-fractionation Homo sapiens
42 SFXN2  
Affinity Capture-MS Homo sapiens
43 C11orf42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ATG7 10533
Co-fractionation Homo sapiens
45 ZNF202  
Two-hybrid Homo sapiens
46 CTPS1 1503
Co-fractionation Homo sapiens
47 RAB35 11021
Proximity Label-MS Homo sapiens
48 CSNK2B 1460
Two-hybrid Homo sapiens
49 SNX4 8723
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
50 KIF14 9928
Affinity Capture-MS Homo sapiens
51 ENOPH1 58478
Co-fractionation Homo sapiens
52 PDAP1 11333
Co-fractionation Homo sapiens
53 C1orf122  
Two-hybrid Homo sapiens
54 SNRNP70 6625
Cross-Linking-MS (XL-MS) Homo sapiens
55 IKBIP 121457
Affinity Capture-MS Homo sapiens
56 ACVR2B  
Affinity Capture-Western Homo sapiens
57 PARK2  
Affinity Capture-MS Homo sapiens
58 PDGFRB 5159
Affinity Capture-Western Homo sapiens
59 SHMT1 6470
Co-fractionation Homo sapiens
60 RBM4B  
Co-fractionation Homo sapiens
61 GNAT2 2780
Affinity Capture-MS Homo sapiens
62 RAB4A 5867
Proximity Label-MS Homo sapiens
63 DNAJC5 80331
Proximity Label-MS Homo sapiens
64 ACVR1B 91
Affinity Capture-Western Homo sapiens
65 CDH1 999
Proximity Label-MS Homo sapiens
66 CYB5A 1528
Affinity Capture-MS Homo sapiens
67 PMAIP1  
Affinity Capture-MS Homo sapiens
68 NTRK1 4914
Affinity Capture-MS Homo sapiens
69 SAR1B 51128
Affinity Capture-MS Homo sapiens
70 SPATA2  
Affinity Capture-MS Homo sapiens
71 DCTN1 1639
Co-fractionation Homo sapiens
72 PEX14 5195
Proximity Label-MS Homo sapiens
73 STX6 10228
Proximity Label-MS Homo sapiens
74 TPP2 7174
Affinity Capture-MS Homo sapiens
75 FNBP1 23048
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
76 TRIM17  
Affinity Capture-MS Homo sapiens
77 YIF1A 10897
Two-hybrid Homo sapiens
78 PPM1G 5496
Cross-Linking-MS (XL-MS) Homo sapiens
79 DNAJB11 51726
Affinity Capture-MS Homo sapiens
80 WDFY1 57590
Co-fractionation Homo sapiens
81 POTEB3 102724631
Affinity Capture-MS Homo sapiens
82 SNX1 6642
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
83 RNF40 9810
Co-fractionation Homo sapiens
84 FAM129B 64855
Co-fractionation Homo sapiens
85 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 C10orf107  
Affinity Capture-MS Homo sapiens
87 KRT18 3875
Proximity Label-MS Homo sapiens
88 RAB5A 5868
Proximity Label-MS Homo sapiens
89 EGLN3  
Affinity Capture-MS Homo sapiens
90 SNX27 81609
Co-fractionation Homo sapiens
91 MTIF3  
Two-hybrid Homo sapiens
92 RAB11A 8766
Proximity Label-MS Homo sapiens
93 TBC1D5 9779
Co-fractionation Homo sapiens
94 PDGFRA 5156
Affinity Capture-Western Homo sapiens
95 GATS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 CTTN 2017
Co-fractionation Homo sapiens
97 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 HOOK3 84376
Proximity Label-MS Homo sapiens
99 ARF6 382
Proximity Label-MS Homo sapiens
100 FAM90A1  
Affinity Capture-MS Homo sapiens
101 FOXL1  
Affinity Capture-MS Homo sapiens
102 ANP32E 81611
Co-fractionation Homo sapiens
103 TBCD 6904
Co-fractionation Homo sapiens
104 SEC24A 10802
Co-fractionation Homo sapiens
105 ERP44 23071
Co-fractionation Homo sapiens
106 AURKB 9212
Affinity Capture-MS Homo sapiens
107 LEPR 3953
Affinity Capture-Western Homo sapiens
108 DEFB136  
Affinity Capture-MS Homo sapiens
109 NRBP1 29959
Affinity Capture-MS Homo sapiens
110 SLC12A3 6559
Affinity Capture-MS Homo sapiens
111 STAT1 6772
Co-fractionation Homo sapiens
112 RPA3 6119
Proximity Label-MS Homo sapiens
113 IGFBPL1  
Affinity Capture-MS Homo sapiens
114 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
115 RAB9A 9367
Proximity Label-MS Homo sapiens
116 ZDHHC18 84243
Affinity Capture-MS Homo sapiens
117 PLIN3 10226
Two-hybrid Homo sapiens
118 TSN 7247
Co-fractionation Homo sapiens
119 DDB1 1642
Co-fractionation Homo sapiens
120 HYOU1 10525
Affinity Capture-MS Homo sapiens
121 PARP1 142
Proximity Label-MS Homo sapiens
122 KIAA0368 23392
Affinity Capture-MS Homo sapiens
123 FSCN1 6624
Affinity Capture-MS Homo sapiens
124 KRT19 3880
Proximity Label-MS Homo sapiens
125 FAM114A2  
Co-fractionation Homo sapiens
126 RTN4 57142
Co-fractionation Homo sapiens
127 SNX5 27131
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
128 CCDC8  
Affinity Capture-MS Homo sapiens
129 ASUN  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
130 ZRANB1 54764
Affinity Capture-MS Homo sapiens
131 TMEM239  
Two-hybrid Homo sapiens
132 MRPL42  
Affinity Capture-MS Homo sapiens
133 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
134 ANKFY1 51479
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
135 LAMTOR1 55004
Proximity Label-MS Homo sapiens
136 DUSP10 11221
Two-hybrid Homo sapiens
137 USP46 64854
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 TP53 7157
Affinity Capture-MS Homo sapiens
139 RUVBL2 10856
Co-fractionation Homo sapiens
140 HENMT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 INSR 3643
Affinity Capture-Western Homo sapiens
142 ZDHHC23  
Affinity Capture-MS Homo sapiens
143 CUX2 23316
Affinity Capture-MS Homo sapiens
144 KLHL1  
Affinity Capture-MS Homo sapiens
145 SDF2L1 23753
Affinity Capture-MS Homo sapiens
146 VPS26B 112936
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
147 H3F3A 3020
Co-fractionation Homo sapiens
148 B3GAT1  
Proximity Label-MS Homo sapiens
149 RAB7A 7879
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
150 DNM3 26052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 C1orf87  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
152 METTL3  
Co-fractionation Homo sapiens
153 SERBP1 26135
Affinity Capture-MS Homo sapiens
154 PRUNE2  
Affinity Capture-MS Homo sapiens
155 RAB5C 5878
Proximity Label-MS Homo sapiens
156 BMP6  
Affinity Capture-MS Homo sapiens
157 DLD 1738
Affinity Capture-MS Homo sapiens
158 C17orf59 54785
Proximity Label-MS Homo sapiens
159 PAK6 56924
Two-hybrid Homo sapiens
160 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here