Gene description for SNX5
Gene name sorting nexin 5
Gene symbol SNX5
Other names/aliases -
Species Homo sapiens
 Database cross references - SNX5
ExoCarta ExoCarta_27131
Vesiclepedia VP_27131
Entrez Gene 27131
HGNC 14969
MIM 605937
UniProt Q9Y5X3  
 SNX5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SNX5
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-5-phosphate binding GO:0010314 IEA
    D1 dopamine receptor binding GO:0031748 IEA
    dynactin binding GO:0034452 IBA
    dynactin binding GO:0034452 IDA
    phosphatidylinositol binding GO:0035091 IBA
    phosphatidylinositol binding GO:0035091 IDA
    phosphatidylinositol binding GO:0035091 ISS
    cadherin binding GO:0045296 HDA
    phosphatidylinositol-4-phosphate binding GO:0070273 IEA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IEA
Biological Process
    intracellular protein transport GO:0006886 IEA
    pinocytosis GO:0006907 IBA
    pinocytosis GO:0006907 IDA
    epidermal growth factor catabolic process GO:0007174 IEA
    regulation of macroautophagy GO:0016241 NAS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IMP
    retrograde transport, endosome to Golgi GO:0042147 NAS
    negative regulation of blood pressure GO:0045776 IEA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of insulin receptor signaling pathway GO:0046628 IMP
Subcellular Localization
    ruffle GO:0001726 IEA
    phagocytic cup GO:0001891 IEA
    endosome GO:0005768 IBA
    cytosol GO:0005829 IDA
    brush border GO:0005903 IEA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    retromer complex GO:0030904 IDA
    retromer, tubulation complex GO:0030905 NAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    macropinocytic cup GO:0070685 IDA
    tubular endosome GO:0097422 IDA
    cytoplasmic side of early endosome membrane GO:0098559 IDA
 Experiment description of studies that identified SNX5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SNX5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GAGE1  
Affinity Capture-MS Homo sapiens
2 OCLN 100506658
Proximity Label-MS Homo sapiens
3 LAMP3  
Proximity Label-MS Homo sapiens
4 GJA1 2697
Proximity Label-MS Homo sapiens
5 PIP5K1C  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 NTNG1  
Affinity Capture-MS Homo sapiens
7 LAMP2 3920
Proximity Label-MS Homo sapiens
8 CCDC102B  
Affinity Capture-MS Homo sapiens
9 VPS26A 9559
Co-fractionation Homo sapiens
10 ZBTB3  
Affinity Capture-MS Homo sapiens
11 C1orf87  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GTF2E2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 GNAO1 2775
Affinity Capture-MS Homo sapiens
14 KLHL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FAM221A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ARID4B  
Affinity Capture-MS Homo sapiens
17 SEMA4C 54910
Affinity Capture-MS Homo sapiens
18 TOM1 10043
Affinity Capture-MS Homo sapiens
19 Mib1  
Two-hybrid Mus musculus
20 PRKRA 8575
Two-hybrid Homo sapiens
21 C11orf42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FAM124A  
Affinity Capture-MS Homo sapiens
23 SNX27 81609
Affinity Capture-MS Homo sapiens
24 SARS 6301
Co-fractionation Homo sapiens
25 LAMTOR1 55004
Proximity Label-MS Homo sapiens
26 CLTCL1 8218
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
27 CCDC114  
Affinity Capture-MS Homo sapiens
28 NUP155 9631
Co-fractionation Homo sapiens
29 MIB1 57534
Two-hybrid Homo sapiens
30 GNAT2 2780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 DNAJC5 80331
Proximity Label-MS Homo sapiens
32 CDH1 999
Proximity Label-MS Homo sapiens
33 RPA2 6118
Proximity Label-MS Homo sapiens
34 SIPA1L2  
Affinity Capture-MS Homo sapiens
35 OGT 8473
Reconstituted Complex Homo sapiens
36 NPTN 27020
Affinity Capture-MS Homo sapiens
37 MLX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SDF2L1 23753
Affinity Capture-MS Homo sapiens
39 ELOVL5 60481
Proximity Label-MS Homo sapiens
40 DNAJB11 51726
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
41 HCLS1 3059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 MESDC2 23184
Two-hybrid Homo sapiens
43 SNX1 6642
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 LAPTM4B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
45 AQP2 359
Two-hybrid Homo sapiens
46 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 BLVRA 644
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RAB5A 5868
Proximity Label-MS Homo sapiens
49 HMOX2 3163
Two-hybrid Homo sapiens
50 LAMP1 3916
Proximity Label-MS Homo sapiens
51 ATG12  
Proximity Label-MS Homo sapiens
52 HSD3B7 80270
Proximity Label-MS Homo sapiens
53 ELAC2 60528
Co-fractionation Homo sapiens
54 ARF6 382
Proximity Label-MS Homo sapiens
55 FAM90A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 ATP1B3 483
Affinity Capture-MS Homo sapiens
57 DEFB136  
Affinity Capture-MS Homo sapiens
58 FANCA  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 SLC12A3 6559
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 IGFBPL1  
Affinity Capture-MS Homo sapiens
62 HYOU1 10525
Affinity Capture-MS Homo sapiens
63 CLTC 1213
Two-hybrid Homo sapiens
64 STK4 6789
Proximity Label-MS Homo sapiens
65 ANKFY1 51479
Proximity Label-MS Homo sapiens
66 ITSN1 6453
Two-hybrid Homo sapiens
67 STAC2  
Affinity Capture-MS Homo sapiens
68 CCDC102A 92922
Affinity Capture-MS Homo sapiens
69 TEX28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CDC37 11140
Two-hybrid Homo sapiens
71 BOP1 23246
Co-fractionation Homo sapiens
72 PFDN1 5201
Two-hybrid Homo sapiens
73 CEACAM16  
Affinity Capture-MS Homo sapiens
74 ARFIP2 23647
Co-fractionation Homo sapiens
75 CUX2 23316
Affinity Capture-MS Homo sapiens
76 APPBP2  
Two-hybrid Homo sapiens
77 XPO5 57510
Co-fractionation Homo sapiens
78 SPANXB1  
Affinity Capture-MS Homo sapiens
79 LRRC61 65999
Proximity Label-MS Homo sapiens
80 DNM3 26052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 RAB11A 8766
Proximity Label-MS Homo sapiens
82 BIN3 55909
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 SERBP1 26135
Affinity Capture-MS Homo sapiens
84 SNX2 6643
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
85 GAS2L1 10634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 VPS33B 26276
Co-fractionation Homo sapiens
87 SNX3 8724
Co-fractionation Homo sapiens
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