Gene description for ARFIP2
Gene name ADP-ribosylation factor interacting protein 2
Gene symbol ARFIP2
Other names/aliases POR1
Species Homo sapiens
 Database cross references - ARFIP2
ExoCarta ExoCarta_23647
Vesiclepedia VP_23647
Entrez Gene 23647
HGNC 17160
MIM 601638
UniProt P53365  
 ARFIP2 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ARFIP2
Molecular Function
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 TAS
    phospholipid binding GO:0005543 IBA
    protein domain specific binding GO:0019904 IEA
    GTP-dependent protein binding GO:0030742 IPI
    small GTPase binding GO:0031267 TAS
    identical protein binding GO:0042802 IPI
    cadherin binding GO:0045296 HDA
    phosphatidylinositol-4-phosphate binding GO:0070273 IDA
    membrane curvature sensor activity GO:0140090 IDA
Biological Process
    mitophagy GO:0000423 IMP
    intracellular protein transport GO:0006886 IBA
    small GTPase-mediated signal transduction GO:0007264 TAS
    lamellipodium assembly GO:0030032 TAS
    actin cytoskeleton organization GO:0030036 IMP
    ruffle organization GO:0031529 TAS
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 IBA
    protein localization to phagophore assembly site GO:0034497 IMP
Subcellular Localization
    ruffle GO:0001726 IMP
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 IDA
    trans-Golgi network membrane GO:0032588 IBA
    trans-Golgi network membrane GO:0032588 IDA
 Experiment description of studies that identified ARFIP2 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ARFIP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCAR1 55749
Affinity Capture-MS Homo sapiens
2 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CALML3 810
Affinity Capture-MS Homo sapiens
4 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
5 REL 5966
Two-hybrid Homo sapiens
6 NUP62 23636
Two-hybrid Homo sapiens
7 ARF3 377
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
8 GSG1  
Two-hybrid Homo sapiens
9 CALM1 801
Affinity Capture-MS Homo sapiens
10 PBX3  
Two-hybrid Homo sapiens
11 APP 351
Reconstituted Complex Homo sapiens
12 TRIM54  
Two-hybrid Homo sapiens
13 B3GAT1  
Proximity Label-MS Homo sapiens
14 SMG9 56006
Two-hybrid Homo sapiens
15 HGS 9146
Two-hybrid Homo sapiens
16 STAT3 6774
Two-hybrid Homo sapiens
17 DGAT2L6  
Two-hybrid Homo sapiens
18 DDOST 1650
Affinity Capture-MS Homo sapiens
19 SNX1 6642
Two-hybrid Homo sapiens
20 XYLT1 64131
Affinity Capture-MS Homo sapiens
21 STX6 10228
Proximity Label-MS Homo sapiens
22 FSD2 123722
Two-hybrid Homo sapiens
23 ARL1 400
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
24 SDCBP 6386
Two-hybrid Homo sapiens
25 SARS 6301
Co-fractionation Homo sapiens
26 LAMTOR1 55004
Proximity Label-MS Homo sapiens
27 IFT20 90410
Affinity Capture-MS Homo sapiens
28 RAB4A 5867
Proximity Label-MS Homo sapiens
29 PFKM 5213
Co-fractionation Homo sapiens
30 NEURL1  
Co-fractionation Homo sapiens
31 GOLGA2 2801
Two-hybrid Homo sapiens
32 ITGB3BP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 DTNBP1  
Two-hybrid Homo sapiens
35 CDH1 999
Proximity Label-MS Homo sapiens
36 C16orf58 64755
Affinity Capture-MS Homo sapiens
37 BICD2 23299
Proximity Label-MS Homo sapiens
38 NRDE2  
Two-hybrid Homo sapiens
39 ELOVL5 60481
Proximity Label-MS Homo sapiens
40 PKNOX2  
Two-hybrid Homo sapiens
41 SCAMP5  
Two-hybrid Homo sapiens
42 REEP5 7905
Two-hybrid Homo sapiens
43 DUSP22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SOCS2  
Two-hybrid Homo sapiens
45 CEP63  
Two-hybrid Homo sapiens
46 TMEM255B  
Two-hybrid Homo sapiens
47 ARF6 382
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
48 METTL3  
Affinity Capture-MS Homo sapiens
49 ARFIP1 27236
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SYP  
Two-hybrid Homo sapiens
51 KRT15 3866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 NDRG4 65009
Two-hybrid Homo sapiens
53 CMTM5  
Two-hybrid Homo sapiens
54 ING5  
Two-hybrid Homo sapiens
55 HSPA1A 3303
Affinity Capture-MS Homo sapiens
56 BICD1 636
Proximity Label-MS Homo sapiens
57 PLP2 5355
Two-hybrid Homo sapiens
58 USHBP1  
Two-hybrid Homo sapiens
59 AKT1 207
Two-hybrid Homo sapiens
60 RAC2 5880
Two-hybrid Homo sapiens
61 KRT16 3868
Two-hybrid Homo sapiens
62 SNX5 27131
Co-fractionation Homo sapiens
63 TTPA  
Two-hybrid Homo sapiens
64 PPIF 10105
Two-hybrid Homo sapiens
65 LY6D 8581
Two-hybrid Homo sapiens
66 HAUS1  
Two-hybrid Homo sapiens
67 ITSN1 6453
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
68 BLOC1S2 282991
Two-hybrid Homo sapiens
69 RND1  
Two-hybrid Homo sapiens
70 ARF5 381
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
71 MAL2 114569
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 DMAP1 55929
Two-hybrid Homo sapiens
73 C19orf25  
Two-hybrid Homo sapiens
74 ARFIP2 23647
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 ARF4 378
Proximity Label-MS Homo sapiens
76 PDLIM5 10611
Co-fractionation Homo sapiens
77 ING1  
Affinity Capture-MS Homo sapiens
78 CDR1  
Two-hybrid Homo sapiens
79 MOV10 4343
Affinity Capture-RNA Homo sapiens
80 MIEF2  
Two-hybrid Homo sapiens
81 RAB7A 7879
Proximity Label-MS Homo sapiens
82 ARF1 375
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
83 RAC3 5881
Two-hybrid Homo sapiens
84 TMEM31  
Affinity Capture-MS Homo sapiens
85 RAC1 5879
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
86 FADS6  
Two-hybrid Homo sapiens
87 CDKN2A 1029
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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