Gene description for NDRG4
Gene name NDRG family member 4
Gene symbol NDRG4
Other names/aliases BDM1
SMAP-8
SMAP8
Species Homo sapiens
 Database cross references - NDRG4
ExoCarta ExoCarta_65009
Vesiclepedia VP_65009
Entrez Gene 65009
HGNC 14466
MIM 614463
UniProt Q9ULP0  
 NDRG4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDRG4
Molecular Function
    molecular_function GO:0003674 ND
    protein binding GO:0005515 IPI
Biological Process
    heart looping GO:0001947 ISS
    signal transduction GO:0007165 IBA
    visual learning GO:0008542 IEA
    negative regulation of platelet-derived growth factor receptor signaling pathway GO:0010642 IDA
    positive regulation of neuron projection development GO:0010976 ISS
    negative regulation of smooth muscle cell migration GO:0014912 IDA
    cell differentiation GO:0030154 NAS
    embryonic heart tube development GO:0035050 ISS
    vesicle docking GO:0048278 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 IDA
    cardiac muscle cell proliferation GO:0060038 ISS
    cell migration involved in heart development GO:0060973 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    regulation of endocytic recycling GO:2001135 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum membrane GO:0005789 ISS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    basolateral plasma membrane GO:0016323 IDA
    cell projection membrane GO:0031253 IEA
 Experiment description of studies that identified NDRG4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDRG4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 UFSP2 55325
Affinity Capture-MS Homo sapiens
3 GPM6A 2823
Affinity Capture-MS Homo sapiens
4 LIME1 54923
Two-hybrid Homo sapiens
5 PICK1  
Affinity Capture-MS Homo sapiens
6 APLNR  
Two-hybrid Homo sapiens
7 SGK1  
Two-hybrid Homo sapiens
8 PRAF2 11230
Two-hybrid Homo sapiens
9 ATP5J 522
Two-hybrid Homo sapiens
10 TP53 7157
Affinity Capture-Western Homo sapiens
11 SIN3A  
Two-hybrid Homo sapiens
12 PRNP 5621
Affinity Capture-MS Homo sapiens
13 LRRC59 55379
Two-hybrid Homo sapiens
14 SDR16C5  
Two-hybrid Homo sapiens
15 SFT2D2 375035
Two-hybrid Homo sapiens
16 THAP4 51078
Two-hybrid Homo sapiens
17 MTIF3  
Two-hybrid Homo sapiens
18 LEUTX  
Two-hybrid Homo sapiens
19 MAL2 114569
Two-hybrid Homo sapiens
20 ERGIC3 51614
Two-hybrid Homo sapiens
21 CACFD1  
Two-hybrid Homo sapiens
22 ARL6IP1 23204
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TMEM208  
Two-hybrid Homo sapiens
25 SYP  
Two-hybrid Homo sapiens
26 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 CPLX2 10814
Two-hybrid Homo sapiens
28 CDR1  
Two-hybrid Homo sapiens
29 RABAC1 10567
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 YAP1 10413
Affinity Capture-MS Homo sapiens
31 CMTM5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SMPD2 6610
Two-hybrid Homo sapiens
34 COQ3  
Two-hybrid Homo sapiens
35 PLP2 5355
Two-hybrid Homo sapiens
36 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CIDEB  
Two-hybrid Homo sapiens
38 FXYD3 5349
Affinity Capture-MS Homo sapiens
39 ARFIP2 23647
Two-hybrid Homo sapiens
40 DGAT2L6  
Two-hybrid Homo sapiens
41 YIF1A 10897
Two-hybrid Homo sapiens
42 SYNGR3  
Two-hybrid Homo sapiens
43 REEP6  
Two-hybrid Homo sapiens
44 FXYD1 5348
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NDRG4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here