Experiment description of studies that identified THAP4 in exosomes

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for THAP4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 TMEM60   Two-hybrid Homo sapiens 2 SLC30A8   Two-hybrid Homo sapiens 3 LAT 27040 Two-hybrid Homo sapiens 4 CMTM7   Two-hybrid Homo sapiens 5 GFER   Affinity Capture-MS Homo sapiens 6 SERPINA12   Affinity Capture-MS Homo sapiens 7 TMEM107 84314 Two-hybrid Homo sapiens 8 AIFM1 9131 Affinity Capture-MS Homo sapiens 9 HSPA8 3312 Affinity Capture-MS Homo sapiens 10 YIPF6 286451 Two-hybrid Homo sapiens 11 CPNE7 27132 Affinity Capture-MS Homo sapiens 12 RTP2   Two-hybrid Homo sapiens 13 ANKRD46 157567 Two-hybrid Homo sapiens 14 CPNE2 221184 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 15 MAL2 114569 Two-hybrid Homo sapiens 16 CDSN 1041 Affinity Capture-MS Homo sapiens 17 MS4A1 931 Two-hybrid Homo sapiens 18 ACTBL2 345651 Affinity Capture-MS Homo sapiens 19 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 20 AIG1   Two-hybrid Homo sapiens 21 TGM1 7051 Affinity Capture-MS Homo sapiens 22 IGFBP5 3488 Two-hybrid Homo sapiens 23 HSP90AA1 3320 Affinity Capture-Luminescence Homo sapiens 24 LOR   Affinity Capture-MS Homo sapiens 25 ALOX12B   Affinity Capture-MS Homo sapiens 26 CYP4F2   Two-hybrid Homo sapiens 27 APP 351 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 28 TRIM54   Two-hybrid Homo sapiens 29 MAPK6   Two-hybrid Homo sapiens 30 HAL 3034 Affinity Capture-MS Homo sapiens 31 SYP   Two-hybrid Homo sapiens 32 AGTRAP 57085 Two-hybrid Homo sapiens 33 TMEM86B   Two-hybrid Homo sapiens 34 CHCHD4   Affinity Capture-MS Homo sapiens 35 ARG1 383 Affinity Capture-MS Homo sapiens 36 NDRG4 65009 Two-hybrid Homo sapiens 37 CORO2A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 BSND   Two-hybrid Homo sapiens 39 THAP4 51078 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 40 HSPA2 3306 Affinity Capture-MS Homo sapiens 41 COL4A5   Two-hybrid Homo sapiens 42 CMTM5   Two-hybrid Homo sapiens 43 GPR37L1   Two-hybrid Homo sapiens 44 GIMAP1   Two-hybrid Homo sapiens 45 CD81 975 Two-hybrid Homo sapiens 46 TMEM86A   Two-hybrid Homo sapiens 47 ACTB 60 Affinity Capture-MS Homo sapiens 48 PLP2 5355 Two-hybrid Homo sapiens 49 CST6   Affinity Capture-MS Homo sapiens 50 CACNG1   Two-hybrid Homo sapiens 51 TGM3 7053 Affinity Capture-MS Homo sapiens 52 SERPINB7 8710 Affinity Capture-MS Homo sapiens 53 ATRX 546 Affinity Capture-MS Homo sapiens 54 DGAT2L6   Two-hybrid Homo sapiens 55 RMDN2 151393 Two-hybrid Homo sapiens 56 LPAR3   Two-hybrid Homo sapiens 57 AMD1   Two-hybrid Homo sapiens 58 TTPA   Two-hybrid Homo sapiens 59 TNFRSF10D 8793 Two-hybrid Homo sapiens 60 CEBPA   Proximity Label-MS Homo sapiens View the network

Pathways in which THAP4 is involved

No pathways found