Gene description for IGFBP5
Gene name insulin-like growth factor binding protein 5
Gene symbol IGFBP5
Other names/aliases IBP5
Species Homo sapiens
 Database cross references - IGFBP5
ExoCarta ExoCarta_3488
Vesiclepedia VP_3488
Entrez Gene 3488
HGNC 5474
MIM 146734
UniProt P24593  
 IGFBP5 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for IGFBP5
Molecular Function
    fibronectin binding GO:0001968 IBA
    protein binding GO:0005515 IPI
    insulin-like growth factor I binding GO:0031994 IBA
    insulin-like growth factor I binding GO:0031994 IPI
    insulin-like growth factor II binding GO:0031995 IBA
    receptor ligand activity GO:0048018 IDA
Biological Process
    regulation of cell growth GO:0001558 IEA
    osteoblast differentiation GO:0001649 IEA
    signal transduction GO:0007165 NAS
    female pregnancy GO:0007565 IEA
    cell population proliferation GO:0008283 IDA
    negative regulation of smooth muscle cell migration GO:0014912 IDA
    negative regulation of translation GO:0017148 IDA
    negative regulation of cell migration GO:0030336 IDA
    hair follicle morphogenesis GO:0031069 IEA
    intracellular signal transduction GO:0035556 IEA
    glucose homeostasis GO:0042593 IEA
    regulation of insulin-like growth factor receptor signaling pathway GO:0043567 IBA
    positive regulation of insulin-like growth factor receptor signaling pathway GO:0043568 IEA
    negative regulation of insulin-like growth factor receptor signaling pathway GO:0043569 IDA
    type B pancreatic cell proliferation GO:0044342 IEA
    negative regulation of osteoblast differentiation GO:0045668 IEA
    negative regulation of growth GO:0045926 IEA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    lung alveolus development GO:0048286 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 IDA
    striated muscle cell differentiation GO:0051146 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    mammary gland involution GO:0060056 IEA
    response to growth hormone GO:0060416 ISS
    cellular response to cAMP GO:0071320 IDA
    cellular response to organic cyclic compound GO:0071407 IDA
    negative regulation of muscle tissue development GO:1901862 IEA
    negative regulation of skeletal muscle hypertrophy GO:1904205 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IGI
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 IGI
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IC
    nucleus GO:0005634 IEA
    endoplasmic reticulum lumen GO:0005788 TAS
    insulin-like growth factor binding protein complex GO:0016942 IC
    insulin-like growth factor ternary complex GO:0042567 IDA
 Experiment description of studies that identified IGFBP5 in exosomes
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for IGFBP5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLDN7 1366
Two-hybrid Homo sapiens
2 GPR151  
Two-hybrid Homo sapiens
3 SLC10A6  
Two-hybrid Homo sapiens
4 CHODL 140578
Two-hybrid Homo sapiens
5 ATE1 11101
Affinity Capture-MS Homo sapiens
6 KIAA2013 90231
Two-hybrid Homo sapiens
7 FCRL3  
Two-hybrid Homo sapiens
8 SYNE4  
Two-hybrid Homo sapiens
9 HRAS 3265
Affinity Capture-MS Homo sapiens
10 MFSD5  
Two-hybrid Homo sapiens
11 EPS8 2059
Affinity Capture-MS Homo sapiens
12 IGFALS 3483
Reconstituted Complex Homo sapiens
13 MS4A3  
Two-hybrid Homo sapiens
14 AMIGO1  
Two-hybrid Homo sapiens
15 TMEM179B 374395
Two-hybrid Homo sapiens
16 TMPRSS2 7113
Two-hybrid Homo sapiens
17 SLC13A4  
Two-hybrid Homo sapiens
18 C3 718
Cross-Linking-MS (XL-MS) Homo sapiens
19 GJB3  
Two-hybrid Homo sapiens
20 TSPO2  
Two-hybrid Homo sapiens
21 IGF2 3481
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CLDN6 9074
Two-hybrid Homo sapiens
23 CLDN9  
Two-hybrid Homo sapiens
24 GPX8 493869
Two-hybrid Homo sapiens
25 TMEM106A  
Two-hybrid Homo sapiens
26 MS4A7  
Two-hybrid Homo sapiens
27 PLCB3 5331
Affinity Capture-MS Homo sapiens
28 TMEM106C 79022
Two-hybrid Homo sapiens
29 ADAM12 8038
Biochemical Activity Homo sapiens
30 VTN 7448
Reconstituted Complex Homo sapiens
31 RPP14  
Affinity Capture-MS Homo sapiens
32 IGHA2 3494
Affinity Capture-MS Homo sapiens
33 TSPAN12  
Two-hybrid Homo sapiens
34 BAG6 7917
Two-hybrid Homo sapiens
35 MPL 4352
Affinity Capture-MS Homo sapiens
36 ANKS6 203286
Two-hybrid Homo sapiens
37 CD53 963
Two-hybrid Homo sapiens
38 SLC41A2 84102
Two-hybrid Homo sapiens
39 RHCG 51458
Two-hybrid Homo sapiens
40 THBS1 7057
Reconstituted Complex Homo sapiens
41 CREB3  
Two-hybrid Homo sapiens
42 PDZK1IP1 10158
Two-hybrid Homo sapiens
43 TMEM56  
Two-hybrid Homo sapiens
44 MFF 56947
Two-hybrid Homo sapiens
45 GJB5  
Two-hybrid Homo sapiens
46 CCDC12  
Affinity Capture-MS Homo sapiens
47 TMEM52B 120939
Two-hybrid Homo sapiens
48 GJA8  
Two-hybrid Homo sapiens
49 LAPTM4B  
Two-hybrid Homo sapiens
50 AQP2 359
Two-hybrid Homo sapiens
51 FNDC9  
Two-hybrid Homo sapiens
52 GJA5 2702
Two-hybrid Homo sapiens
53 TMEM45A  
Two-hybrid Homo sapiens
54 NBEA  
Affinity Capture-MS Homo sapiens
55 SLC41A3 54946
Two-hybrid Homo sapiens
56 GPX1 2876
Affinity Capture-MS Homo sapiens
57 PROKR2  
Two-hybrid Homo sapiens
58 ERGIC3 51614
Two-hybrid Homo sapiens
59 CUL3 8452
Affinity Capture-MS Homo sapiens
60 IGF1  
Reconstituted Complex Homo sapiens
61 SERPINE1 5054
Affinity Capture-Western Homo sapiens
62 HSD17B13  
Two-hybrid Homo sapiens
63 PPP2CA 5515
Affinity Capture-MS Homo sapiens
64 GPR152  
Two-hybrid Homo sapiens
65 FAM209A 200232
Two-hybrid Homo sapiens
66 GPR42  
Two-hybrid Homo sapiens
67 TMEM237 65062
Two-hybrid Homo sapiens
68 GNB2 2783
Affinity Capture-MS Homo sapiens
69 RPP40 10799
Affinity Capture-MS Homo sapiens
70 LHFPL1  
Two-hybrid Homo sapiens
71 AHNAK2 113146
Two-hybrid Homo sapiens
72 CLDN20  
Two-hybrid Homo sapiens
73 FHL2 2274
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
74 CXCR1  
Two-hybrid Homo sapiens
75 TMX2 51075
Two-hybrid Homo sapiens
76 AQP9  
Two-hybrid Homo sapiens
77 CLDN2 9075
Two-hybrid Homo sapiens
78 ASGR1 432
Two-hybrid Homo sapiens
79 LDLRAD1  
Two-hybrid Homo sapiens
80 KLHL8  
Affinity Capture-MS Homo sapiens
81 EIF1AX 1964
Affinity Capture-MS Homo sapiens
82 SPP1 6696
Reconstituted Complex Homo sapiens
83 FBXL16  
Affinity Capture-MS Homo sapiens
84 GPRC5D  
Two-hybrid Homo sapiens
85 LAYN 143903
Two-hybrid Homo sapiens
86 MRPS18B 28973
Two-hybrid Homo sapiens
87 BSND  
Two-hybrid Homo sapiens
88 THAP4 51078
Two-hybrid Homo sapiens
89 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
90 SLC38A1 81539
Two-hybrid Homo sapiens
91 GPR37L1  
Two-hybrid Homo sapiens
92 KCNK5  
Two-hybrid Homo sapiens
93 KRAS 3845
Affinity Capture-MS Homo sapiens
94 APH1A 51107
Two-hybrid Homo sapiens
95 GOLM1 51280
Two-hybrid Homo sapiens
96 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
97 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
98 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
99 CLEC17A  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here