Gene description for ADAM12
Gene name ADAM metallopeptidase domain 12
Gene symbol ADAM12
Other names/aliases ADAM12-OT1
CAR10
MCMP
MCMPMltna
MLTN
MLTNA
Species Homo sapiens
 Database cross references - ADAM12
ExoCarta ExoCarta_8038
Vesiclepedia VP_8038
Entrez Gene 8038
HGNC 190
MIM 602714
UniProt O43184  
 ADAM12 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ADAM12
Molecular Function
    metalloendopeptidase activity GO:0004222 IBA
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 TAS
    SH3 domain binding GO:0017124 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    proteolysis GO:0006508 IBA
    cell adhesion GO:0007155 IEA
    myoblast fusion GO:0007520 TAS
    positive regulation of angiogenesis GO:0045766 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified ADAM12 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ADAM12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT83  
Two-hybrid Homo sapiens
2 KRTAP9-8  
Two-hybrid Homo sapiens
3 KRTAP3-1  
Two-hybrid Homo sapiens
4 LCE5A  
Two-hybrid Homo sapiens
5 GUCD1  
Two-hybrid Homo sapiens
6 KRTAP1-3  
Two-hybrid Homo sapiens
7 IGFBP3 3486
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
8 NOTCH2NL 388677
Two-hybrid Homo sapiens
9 ADAMTSL4  
Two-hybrid Homo sapiens
10 KRTAP10-8  
Two-hybrid Homo sapiens
11 NBPF19  
Two-hybrid Homo sapiens
12 FHL5 9457
Two-hybrid Homo sapiens
13 MAPKBP1  
Two-hybrid Homo sapiens
14 KRTAP1-1  
Two-hybrid Homo sapiens
15 KRTAP5-6  
Two-hybrid Homo sapiens
16 IGFBP5 3488
Biochemical Activity Homo sapiens
17 HOXA1 3198
Two-hybrid Homo sapiens
18 KRTAP5-9  
Two-hybrid Homo sapiens
19 OTX1  
Two-hybrid Homo sapiens
20 KRTAP4-12  
Two-hybrid Homo sapiens
21 ESM1 11082
Two-hybrid Homo sapiens
22 KRTAP6-2  
Two-hybrid Homo sapiens
23 ACTN2 88
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 SPRY4 81848
Two-hybrid Homo sapiens
25 CYSRT1 375791
Two-hybrid Homo sapiens
26 SLC15A2 6565
Two-hybrid Homo sapiens
27 SPRY1 10252
Two-hybrid Homo sapiens
28 YARS2  
Co-fractionation Homo sapiens
29 RECK 8434
Two-hybrid Homo sapiens
30 KRTAP9-2  
Two-hybrid Homo sapiens
31 PIK3R1 5295
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 KRTAP3-2  
Two-hybrid Homo sapiens
33 KRTAP12-2  
Two-hybrid Homo sapiens
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