Gene description for SPRY1
Gene name sprouty homolog 1, antagonist of FGF signaling (Drosophila)
Gene symbol SPRY1
Other names/aliases hSPRY1
Species Homo sapiens
 Database cross references - SPRY1
ExoCarta ExoCarta_10252
Entrez Gene 10252
HGNC 11269
MIM 602465
UniProt O43609  
 SPRY1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 21557262    
 Gene ontology annotations for SPRY1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    organ induction GO:0001759 IEA
    bud elongation involved in lung branching GO:0060449 IEA
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 IEA
    establishment of mitotic spindle orientation GO:0000132 IEA
    negative regulation of neurotrophin TRK receptor signaling pathway GO:0051387 IEA
    negative regulation of MAP kinase activity GO:0043407 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    negative regulation of Ras protein signal transduction GO:0046580 IEA
    metanephros development GO:0001656 IEA
    ureteric bud development GO:0001657 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SPRY1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 114
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors Li Y, Zhang Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SPRY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SPRY1 is involved
PathwayEvidenceSource
EGFR downregulation TAS Reactome





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