Gene description for PRKAB2
Gene name protein kinase, AMP-activated, beta 2 non-catalytic subunit
Gene symbol PRKAB2
Other names/aliases -
Species Homo sapiens
 Database cross references - PRKAB2
ExoCarta ExoCarta_5565
Vesiclepedia VP_5565
Entrez Gene 5565
HGNC 9379
MIM 602741
UniProt O43741  
 PRKAB2 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for PRKAB2
Molecular Function
    AMP-activated protein kinase activity GO:0004679 IDA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
Biological Process
    fatty acid biosynthetic process GO:0006633 IEA
    signal transduction GO:0007165 IBA
    cellular response to nutrient levels GO:0031669 IDA
    cellular response to nutrient levels GO:0031669 NAS
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    nucleotide-activated protein kinase complex GO:0031588 IBA
    nucleotide-activated protein kinase complex GO:0031588 IDA
    nucleotide-activated protein kinase complex GO:0031588 IPI
    nucleotide-activated protein kinase complex GO:0031588 ISS
 Experiment description of studies that identified PRKAB2 in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for PRKAB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 MORN3  
Two-hybrid Homo sapiens
3 NET1 10276
Affinity Capture-MS Homo sapiens
4 ZMYND19  
Two-hybrid Homo sapiens
5 PRKAA1 5562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GATAD2B 57459
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 PPP1R3C  
Two-hybrid Homo sapiens
8 PDE6G  
Two-hybrid Homo sapiens
9 RBPMS 11030
Two-hybrid Homo sapiens
10 CCDC36  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 KRTAP2-3  
Two-hybrid Homo sapiens
12 PDE6H  
Two-hybrid Homo sapiens
13 PSMD11 5717
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 REL 5966
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 MID1IP1  
Affinity Capture-MS Homo sapiens
16 QKI 9444
Two-hybrid Homo sapiens
17 MDFI  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 TCF7L2  
Two-hybrid Homo sapiens
19 FANCI 55215
Affinity Capture-MS Homo sapiens
20 KRTAP5-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 TRIM55  
Two-hybrid Homo sapiens
22 KRTAP1-1  
Two-hybrid Homo sapiens
23 GCN1L1 10985
Affinity Capture-MS Homo sapiens
24 PRKDC 5591
Affinity Capture-MS Homo sapiens
25 TRIM54  
Two-hybrid Homo sapiens
26 RB1CC1 9821
Affinity Capture-MS Homo sapiens
27 MAP3K5 4217
Affinity Capture-MS Homo sapiens
28 GYG1 2992
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KRTAP4-12  
Two-hybrid Homo sapiens
30 SSC4D  
Two-hybrid Homo sapiens
31 RHEBL1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 GFPT1 2673
Affinity Capture-MS Homo sapiens
33 TCF12  
Two-hybrid Homo sapiens
34 TRIM35 23087
Two-hybrid Homo sapiens
35 STIM2 57620
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 RAB3IP  
Two-hybrid Homo sapiens
37 KRTAP2-4  
Two-hybrid Homo sapiens
38 KRT40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 SCRIB 23513
Affinity Capture-MS Homo sapiens
40 CALCOCO2  
Two-hybrid Homo sapiens
41 KLF15  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 IL37  
Two-hybrid Homo sapiens
43 DAO  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 KRTAP10-11  
Two-hybrid Homo sapiens
45 ANAPC11  
Two-hybrid Homo sapiens
46 MAP3K6  
Affinity Capture-MS Homo sapiens
47 ARID5A  
Two-hybrid Homo sapiens
48 GRAPL  
Two-hybrid Homo sapiens
49 YY1AP1 55249
Two-hybrid Homo sapiens
50 KRTAP4-2  
Two-hybrid Homo sapiens
51 CCDC28B  
Two-hybrid Homo sapiens
52 CSNK2B 1460
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 KRTAP17-1  
Two-hybrid Homo sapiens
54 RPH3AL 9501
Two-hybrid Homo sapiens
55 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
56 SERTAD2 9792
Two-hybrid Homo sapiens
57 PSME3 10197
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 C19orf54  
Two-hybrid Homo sapiens
59 GOLGA6L9  
Two-hybrid Homo sapiens
60 ADAMTSL4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 BANP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 ZNF177  
Two-hybrid Homo sapiens
63 NEB 4703
Affinity Capture-MS Homo sapiens
64 POLR3K  
Affinity Capture-MS Homo sapiens
65 ZNF526  
Two-hybrid Homo sapiens
66 SLC9A5  
Affinity Capture-MS Homo sapiens
67 CCDC33 80125
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
68 TRIM42  
Two-hybrid Homo sapiens
69 AES 166
Two-hybrid Homo sapiens
70 PRKAG1 5571
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
71 LRIF1  
Two-hybrid Homo sapiens
72 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 CFP  
Two-hybrid Homo sapiens
74 KRTAP4-5  
Two-hybrid Homo sapiens
75 TRIM14 9830
Two-hybrid Homo sapiens
76 ABHD11 83451
Two-hybrid Homo sapiens
77 ZBTB32  
Two-hybrid Homo sapiens
78 C11orf1  
Two-hybrid Homo sapiens
79 CASP6 839
Two-hybrid Homo sapiens
80 GORASP2 26003
Two-hybrid Homo sapiens
81 LHX3  
Two-hybrid Homo sapiens
82 WBSCR27  
Two-hybrid Homo sapiens
83 MEOX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
84 ULK1  
Affinity Capture-MS Homo sapiens
85 IRAK1BP1 134728
Two-hybrid Homo sapiens
86 ZNF581  
Two-hybrid Homo sapiens
87 GCSAML  
Two-hybrid Homo sapiens
88 CDX4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
89 GET4 51608
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
90 TRIM63  
Two-hybrid Homo sapiens
91 TSR2  
Two-hybrid Homo sapiens
92 KRBOX4  
Two-hybrid Homo sapiens
93 KRTAP10-7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
94 DDAH2 23564
Two-hybrid Homo sapiens
95 ELL2 22936
Two-hybrid Homo sapiens
96 KRTAP9-3  
Two-hybrid Homo sapiens
97 KRTAP9-8  
Two-hybrid Homo sapiens
98 DST 667
Two-hybrid Homo sapiens
99 RNF144B  
Two-hybrid Homo sapiens
100 KATNBL1  
Two-hybrid Homo sapiens
101 KRTAP1-3  
Two-hybrid Homo sapiens
102 OXER1  
Two-hybrid Homo sapiens
103 DOK3 79930
Two-hybrid Homo sapiens
104 IKZF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
105 C11orf85  
Two-hybrid Homo sapiens
106 TRIM10  
Two-hybrid Homo sapiens
107 GYS1 2997
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
109 PRKAA2 5563
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
110 NEBL 10529
Two-hybrid Homo sapiens
111 CASP2  
Two-hybrid Homo sapiens
112 USP54 159195
Two-hybrid Homo sapiens
113 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 PHKG2 5261
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 KRTAP10-3  
Two-hybrid Homo sapiens
116 IKZF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
117 AGL 178
Affinity Capture-MS Homo sapiens
118 TTC23  
Two-hybrid Homo sapiens
119 BAIAP2L1 55971
Affinity Capture-MS Homo sapiens
120 VBP1 7411
Two-hybrid Homo sapiens
121 TGM7  
Two-hybrid Homo sapiens
122 CRX  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
123 RIMBP3 85376
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
124 LZTS2 84445
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
125 GADD45GIP1  
Affinity Capture-MS Homo sapiens
126 DICER1  
Two-hybrid Homo sapiens
127 STX19  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
128 KIAA0907  
Two-hybrid Homo sapiens
129 TADA2A  
Two-hybrid Homo sapiens
130 AVP  
Two-hybrid Homo sapiens
131 TRIM11  
Affinity Capture-MS Homo sapiens
132 FAM208B 54906
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
133 SPRY1 10252
Two-hybrid Homo sapiens
134 AUNIP  
Two-hybrid Homo sapiens
135 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
136 C2orf42  
Two-hybrid Homo sapiens
137 KRTAP9-2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
138 STBD1 8987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 PRKAB2 5565
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
140 ZC2HC1C  
Two-hybrid Homo sapiens
141 PPP1R3B  
Two-hybrid Homo sapiens
142 PFDN5 5204
Two-hybrid Homo sapiens
143 KRTAP10-5  
Two-hybrid Homo sapiens
144 CREB3L1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
145 SMUG1  
Two-hybrid Homo sapiens
146 STX11 8676
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
147 KRTAP3-3  
Two-hybrid Homo sapiens
148 NUTM1  
Two-hybrid Homo sapiens
149 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 TP53BP2  
Two-hybrid Homo sapiens
151 GRN 2896
Two-hybrid Homo sapiens
152 FDX1 2230
Two-hybrid Homo sapiens
153 ZNF236  
Two-hybrid Homo sapiens
154 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
155 SPRY2 10253
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
156 VPS28 51160
Two-hybrid Homo sapiens
157 PRDM14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
158 BHLHB9 80823
Two-hybrid Homo sapiens
159 PIAS2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
160 CYSRT1 375791
Two-hybrid Homo sapiens
161 MCL1 4170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
162 BLZF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
163 KRTAP9-4  
Two-hybrid Homo sapiens
164 CDSN 1041
Two-hybrid Homo sapiens
165 PRKAB1 5564
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
166 PRKAG2 51422
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 UBXN11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
168 DDIT4L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
169 RBM48  
Two-hybrid Homo sapiens
170 ZNF474  
Two-hybrid Homo sapiens
171 ROR2 4920
Two-hybrid Homo sapiens
172 TCF19  
Two-hybrid Homo sapiens
173 NHLRC4  
Two-hybrid Homo sapiens
174 ZNF559-ZNF177 100529215
Two-hybrid Homo sapiens
175 ZFP90  
Two-hybrid Homo sapiens
176 PYGM 5837
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
177 TCF4  
Two-hybrid Homo sapiens
178 MAGED1 9500
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
179 C12orf74  
Two-hybrid Homo sapiens
180 NAB2 4665
Two-hybrid Homo sapiens
181 PYGB 5834
Affinity Capture-MS Homo sapiens
182 C3orf36  
Two-hybrid Homo sapiens
183 ESM1 11082
Two-hybrid Homo sapiens
184 WDR83  
Two-hybrid Homo sapiens
185 TRIB3  
Two-hybrid Homo sapiens
186 FLNC 2318
Two-hybrid Homo sapiens
187 PRKAG3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
188 GNB2L1 10399
Two-hybrid Homo sapiens
189 BEND5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
190 MAST3  
Affinity Capture-MS Homo sapiens
191 Prkaa1  
Affinity Capture-MS Mus musculus
192 MAP3K15  
Affinity Capture-MS Homo sapiens
193 EPM2A  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
194 KCTD5 54442
Two-hybrid Homo sapiens
195 PPP1R16A 84988
Two-hybrid Homo sapiens
196 TNNI1 7135
Two-hybrid Homo sapiens
197 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
198 KRTAP10-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PRKAB2 is involved
PathwayEvidenceSource
Activation of AMPK downstream of NMDARs IEA Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Activation of PPARGC1A (PGC-1alpha) by phosphorylation IEA Reactome
AMPK inhibits chREBP transcriptional activation activity TAS Reactome
AMPK inhibits chREBP transcriptional activation activity IEA Reactome
Autophagy IEA Reactome
Autophagy TAS Reactome
Carnitine shuttle TAS Reactome
Energy dependent regulation of mTOR by LKB1-AMPK TAS Reactome
Fatty acid metabolism TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Integration of energy metabolism TAS Reactome
Integration of energy metabolism IEA Reactome
Lipophagy IEA Reactome
Macroautophagy IEA Reactome
Macroautophagy TAS Reactome
Membrane Trafficking IEA Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of lipids TAS Reactome
Mitochondrial biogenesis IEA Reactome
MTOR signalling TAS Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Post NMDA receptor activation events IEA Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Activity through Phosphorylation TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Selective autophagy IEA Reactome
Signal Transduction TAS Reactome
TP53 Regulates Metabolic Genes TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Translocation of SLC2A4 (GLUT4) to the plasma membrane IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Vesicle-mediated transport IEA Reactome





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