Gene description for RPH3AL
Gene name rabphilin 3A-like (without C2 domains)
Gene symbol RPH3AL
Other names/aliases NOC2
Species Homo sapiens
 Database cross references - RPH3AL
ExoCarta ExoCarta_9501
Vesiclepedia VP_9501
Entrez Gene 9501
HGNC 10296
MIM 604881
UniProt Q9UNE2  
 RPH3AL identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RPH3AL
Molecular Function
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 TAS
    small GTPase binding GO:0031267 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    intracellular protein transport GO:0006886 IEA
    exocytosis GO:0006887 TAS
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    positive regulation of insulin secretion GO:0032024 IEA
    glucose homeostasis GO:0042593 IEA
    negative regulation of G protein-coupled receptor signaling pathway GO:0045744 IEA
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IBA
    calcium-dependent activation of synaptic vesicle fusion GO:0099502 IBA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    transport vesicle membrane GO:0030658 IEA
    synapse GO:0045202 IBA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified RPH3AL in exosomes
1
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RPH3AL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SCNM1  
Two-hybrid Homo sapiens
2 HNRNPK 3190
Two-hybrid Homo sapiens
3 ATPAF2 91647
Two-hybrid Homo sapiens
4 PSMD14 10213
Affinity Capture-MS Homo sapiens
5 MYO15B 80022
Two-hybrid Homo sapiens
6 RAB3GAP1 22930
Reconstituted Complex Homo sapiens
7 SAAL1 113174
Two-hybrid Homo sapiens
8 KHDRBS3  
Two-hybrid Homo sapiens
9 PRKAB2 5565
Two-hybrid Homo sapiens
10 TCEA3  
Two-hybrid Homo sapiens
11 UNC13B 10497
Reconstituted Complex Homo sapiens
12 PNMA3  
Two-hybrid Homo sapiens
13 RAB27A 5873
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
14 MID2 11043
Two-hybrid Homo sapiens
15 C1orf109  
Two-hybrid Homo sapiens
16 PSME3 10197
Two-hybrid Homo sapiens
17 ANKRD11 29123
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RPH3AL is involved
No pathways found





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