Gene description for MID2
Gene name midline 2
Gene symbol MID2
Other names/aliases FXY2
MRX101
RNF60
TRIM1
Species Homo sapiens
 Database cross references - MID2
ExoCarta ExoCarta_11043
Entrez Gene 11043
HGNC 7096
MIM 300204
UniProt Q9UJV3  
 MID2 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for MID2
Molecular Function
    zinc ion binding GO:0008270 IEA
    protein homodimerization activity GO:0042803 IDA
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IEA
    microtubule binding GO:0008017 IMP
    protein heterodimerization activity GO:0046982 IDA
    phosphoprotein binding GO:0051219 IPI
Biological Process
    protein ubiquitination GO:0016567 IEA
    negative regulation of viral release from host cell GO:1902187 IDA
    negative regulation of viral transcription GO:0032897 IDA
    protein localization to microtubule GO:0035372 IMP
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IMP
    negative regulation of viral entry into host cell GO:0046597 IDA
    innate immune response GO:0045087 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IDA
Subcellular Localization
    microtubule GO:0005874 IDA
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified MID2 in exosomes
1
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MID2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIM32 22954
Two-hybrid Homo sapiens
2 TRIM29  
Two-hybrid Homo sapiens
3 N/A  
Two-hybrid Homo sapiens
4 MID1  
Invivo Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 MID2 11043
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
6 IGBP1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MID2 is involved
No pathways found





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