Gene description for CHD2
Gene name chromodomain helicase DNA binding protein 2
Gene symbol CHD2
Other names/aliases EEOC
Species Homo sapiens
 Database cross references - CHD2
ExoCarta ExoCarta_1106
Entrez Gene 1106
HGNC 1917
MIM 602119
UniProt O14647  
 CHD2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for CHD2
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    ATP binding GO:0005524 IEA
    DNA binding GO:0003677 TAS
    core promoter sequence-specific DNA binding GO:0001046 ISS
    ATP-dependent DNA helicase activity GO:0004003 TAS
    protein binding GO:0005515 IPI
    histone binding GO:0042393 ISS
Biological Process
    DNA duplex unwinding GO:0032508 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    cellular response to DNA damage stimulus GO:0006974 IEA
    hematopoietic stem cell differentiation GO:0060218 IEA
    muscle organ development GO:0007517 ISS
    transcription, DNA-templated GO:0006351 IEA
    chromatin modification GO:0016568 IEA
Subcellular Localization
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleolus GO:0005730 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CHD2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CHD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CHD2 is involved
No pathways found





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