Gene description for KIF20A
Gene name kinesin family member 20A
Gene symbol KIF20A
Other names/aliases MKLP2
RAB6KIFL
Species Homo sapiens
 Database cross references - KIF20A
ExoCarta ExoCarta_10112
Vesiclepedia VP_10112
Entrez Gene 10112
HGNC 9787
MIM 605664
UniProt O95235  
 KIF20A identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for KIF20A
Molecular Function
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    ATP hydrolysis activity GO:0016887 IBA
    protein kinase binding GO:0019901 IPI
Biological Process
    mitotic cytokinesis GO:0000281 IMP
    microtubule bundle formation GO:0001578 IDA
    microtubule-based movement GO:0007018 IBA
    protein transport GO:0015031 IEA
    regulation of cytokinesis GO:0032465 IDA
    midbody abscission GO:0061952 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IEA
    spindle GO:0005819 IDA
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    midbody GO:0030496 IDA
    intercellular bridge GO:0045171 IDA
 Experiment description of studies that identified KIF20A in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF20A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 P4HB 5034
Affinity Capture-MS Homo sapiens
4 RALYL 138046
Affinity Capture-MS Homo sapiens
5 GAR1 54433
Affinity Capture-MS Homo sapiens
6 WDR6 11180
Affinity Capture-MS Homo sapiens
7 UTRN 7402
Affinity Capture-MS Homo sapiens
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
9 HDAC2 3066
Affinity Capture-MS Homo sapiens
10 ITIH4 3700
Affinity Capture-MS Homo sapiens
11 SHC1 6464
Affinity Capture-MS Homo sapiens
12 PRPF8 10594
Affinity Capture-MS Homo sapiens
13 PKM 5315
Affinity Capture-MS Homo sapiens
14 PKP2 5318
Affinity Capture-MS Homo sapiens
15 TPM2 7169
Affinity Capture-MS Homo sapiens
16 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
17 GLB1 2720
Affinity Capture-MS Homo sapiens
18 DDX17 10521
Affinity Capture-MS Homo sapiens
19 ACTC1 70
Affinity Capture-MS Homo sapiens
20 BRCA1 672
Affinity Capture-MS Homo sapiens
21 KIF20A 10112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MAD2L1BP  
Affinity Capture-MS Homo sapiens
23 MAP4K4 9448
Affinity Capture-MS Homo sapiens
24 KPNA2 3838
Affinity Capture-MS Homo sapiens
25 CTDP1 9150
Affinity Capture-MS Homo sapiens
26 PRPF40A 55660
Affinity Capture-MS Homo sapiens
27 SNCB 6620
Affinity Capture-MS Homo sapiens
28 GCN1L1 10985
Affinity Capture-MS Homo sapiens
29 PRKDC 5591
Affinity Capture-MS Homo sapiens
30 AKAP8L  
Affinity Capture-MS Homo sapiens
31 RPL22 6146
Affinity Capture-MS Homo sapiens
32 POLH  
Affinity Capture-MS Homo sapiens
33 CPSF2 53981
Affinity Capture-MS Homo sapiens
34 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
35 SNRNP70 6625
Affinity Capture-MS Homo sapiens
36 C9orf89 84270
Affinity Capture-MS Homo sapiens
37 CGN  
Affinity Capture-MS Homo sapiens
38 CAPN12  
Affinity Capture-MS Homo sapiens
39 KIF23 9493
Affinity Capture-MS Homo sapiens
40 RAF1 5894
Affinity Capture-MS Homo sapiens
41 SF3B1 23451
Affinity Capture-MS Homo sapiens
42 PPP1R18 170954
Affinity Capture-MS Homo sapiens
43 STOM 2040
Affinity Capture-MS Homo sapiens
44 SEL1L 6400
Affinity Capture-MS Homo sapiens
45 FLII 2314
Affinity Capture-MS Homo sapiens
46 ZFAT  
Affinity Capture-MS Homo sapiens
47 CIRH1A 84916
Affinity Capture-MS Homo sapiens
48 ABCD3 5825
Affinity Capture-MS Homo sapiens
49 FBL 2091
Affinity Capture-MS Homo sapiens
50 TFIP11  
Two-hybrid Homo sapiens
51 RBFOX3  
Affinity Capture-MS Homo sapiens
52 RPS9 6203
Affinity Capture-MS Homo sapiens
53 ZNF408  
Affinity Capture-MS Homo sapiens
54 POLRMT 5442
Affinity Capture-MS Homo sapiens
55 C19orf33  
Affinity Capture-MS Homo sapiens
56 CLINT1 9685
Affinity Capture-MS Homo sapiens
57 HERC2 8924
Affinity Capture-MS Homo sapiens
58 EIF2B5 8893
Affinity Capture-MS Homo sapiens
59 SNRPD3 6634
Affinity Capture-MS Homo sapiens
60 RPL13AP3 645683
Affinity Capture-MS Homo sapiens
61 PRDX4 10549
Affinity Capture-MS Homo sapiens
62 PKN3 29941
Affinity Capture-MS Homo sapiens
63 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RPS11 6205
Affinity Capture-MS Homo sapiens
65 C5orf30  
Affinity Capture-MS Homo sapiens
66 TRIM4 89122
Affinity Capture-MS Homo sapiens
67 RPL19 6143
Affinity Capture-MS Homo sapiens
68 RBM14 10432
Affinity Capture-MS Homo sapiens
69 MYL6 4637
Affinity Capture-MS Homo sapiens
70 EXOSC9 5393
Affinity Capture-MS Homo sapiens
71 AGO2 27161
Affinity Capture-MS Homo sapiens
72 URB1 9875
Affinity Capture-MS Homo sapiens
73 OOEP  
Affinity Capture-MS Homo sapiens
74 APC2  
Affinity Capture-MS Homo sapiens
75 RBM12B  
Affinity Capture-MS Homo sapiens
76 TRIM7  
Affinity Capture-MS Homo sapiens
77 LIN28B  
Affinity Capture-MS Homo sapiens
78 CAPZA1 829
Affinity Capture-MS Homo sapiens
79 RAB14 51552
Affinity Capture-MS Homo sapiens
80 TOP2B 7155
Affinity Capture-MS Homo sapiens
81 LUZP1 7798
Affinity Capture-MS Homo sapiens
82 PGAM5 192111
Affinity Capture-MS Homo sapiens
83 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
84 SRSF3 6428
Affinity Capture-MS Homo sapiens
85 CECR5 27440
Affinity Capture-MS Homo sapiens
86 TXNRD3NB  
Affinity Capture-MS Homo sapiens
87 CLTCL1 8218
Affinity Capture-MS Homo sapiens
88 TUFM 7284
Affinity Capture-MS Homo sapiens
89 PSIP1 11168
Affinity Capture-MS Homo sapiens
90 YME1L1 10730
Affinity Capture-MS Homo sapiens
91 TPM4 7171
Affinity Capture-MS Homo sapiens
92 UBASH3B 84959
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
93 PABPC1 26986
Affinity Capture-MS Homo sapiens
94 FYN 2534
Affinity Capture-MS Homo sapiens
95 ARPC4 10093
Affinity Capture-MS Homo sapiens
96 MYH9 4627
Affinity Capture-MS Homo sapiens
97 ACTN4 81
Affinity Capture-MS Homo sapiens
98 RPL31 6160
Affinity Capture-MS Homo sapiens
99 CBX5 23468
Affinity Capture-MS Homo sapiens
100 ZEB2  
Affinity Capture-MS Homo sapiens
101 ACADM 34
Affinity Capture-MS Homo sapiens
102 XRCC5 7520
Affinity Capture-MS Homo sapiens
103 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
104 BANF1 8815
Affinity Capture-MS Homo sapiens
105 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
106 ZNF281  
Affinity Capture-MS Homo sapiens
107 WHSC1L1  
Affinity Capture-MS Homo sapiens
108 SON 6651
Affinity Capture-MS Homo sapiens
109 DDX39B 7919
Affinity Capture-MS Homo sapiens
110 FLOT2 2319
Affinity Capture-MS Homo sapiens
111 RPS26P11  
Affinity Capture-MS Homo sapiens
112 VDAC2 7417
Affinity Capture-MS Homo sapiens
113 GTPBP4 23560
Affinity Capture-MS Homo sapiens
114 GYS2 2998
Affinity Capture-MS Homo sapiens
115 LRCH3 84859
Affinity Capture-MS Homo sapiens
116 CD3EAP  
Proximity Label-MS Homo sapiens
117 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
118 REEP5 7905
Affinity Capture-MS Homo sapiens
119 BCLAF1 9774
Affinity Capture-MS Homo sapiens
120 TRIP13 9319
Affinity Capture-MS Homo sapiens
121 PHLPP1  
Proximity Label-MS Homo sapiens
122 SDHAF1  
Affinity Capture-MS Homo sapiens
123 HIST1H2BL 8340
Affinity Capture-MS Homo sapiens
124 HIST1H2AD 3013
Affinity Capture-MS Homo sapiens
125 RAB6A 5870
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
126 BRF1  
Affinity Capture-MS Homo sapiens
127 HERC1 8925
Affinity Capture-MS Homo sapiens
128 FAM83B  
Affinity Capture-MS Homo sapiens
129 HADHB 3032
Affinity Capture-MS Homo sapiens
130 HADHA 3030
Affinity Capture-MS Homo sapiens
131 NOC3L 64318
Affinity Capture-MS Homo sapiens
132 CTAGE15  
Affinity Capture-MS Homo sapiens
133 MRTO4 51154
Affinity Capture-MS Homo sapiens
134 RNF181  
Affinity Capture-MS Homo sapiens
135 TBKBP1  
Affinity Capture-MS Homo sapiens
136 LMNB1 4001
Affinity Capture-MS Homo sapiens
137 ARPC3 10094
Affinity Capture-MS Homo sapiens
138 LCA5L  
Affinity Capture-MS Homo sapiens
139 ERBB2IP 55914
Affinity Capture-MS Homo sapiens
140 LMO7 4008
Affinity Capture-MS Homo sapiens
141 MYH14 79784
Affinity Capture-MS Homo sapiens
142 SETX  
Affinity Capture-MS Homo sapiens
143 NEBL 10529
Affinity Capture-MS Homo sapiens
144 CUL3 8452
Affinity Capture-MS Homo sapiens
145 DES 1674
Affinity Capture-MS Homo sapiens
146 ATAD3C 219293
Affinity Capture-MS Homo sapiens
147 GPNMB 10457
Affinity Capture-MS Homo sapiens
148 EGFL7 51162
Affinity Capture-MS Homo sapiens
149 PHKG2 5261
Affinity Capture-MS Homo sapiens
150 AAAS 8086
Affinity Capture-MS Homo sapiens
151 SYNE2 23224
Affinity Capture-MS Homo sapiens
152 RPL7L1 285855
Affinity Capture-MS Homo sapiens
153 RBBP4 5928
Affinity Capture-MS Homo sapiens
154 NIP7 51388
Affinity Capture-MS Homo sapiens
155 MYO1C 4641
Affinity Capture-MS Homo sapiens
156 COG1 9382
Affinity Capture-MS Homo sapiens
157 WDR43 23160
Affinity Capture-MS Homo sapiens
158 CDK5RAP2 55755
Affinity Capture-MS Homo sapiens
159 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
161 PCBP1 5093
Affinity Capture-MS Homo sapiens
162 GNAS 2778
Affinity Capture-MS Homo sapiens
163 MFAP1  
Affinity Capture-MS Homo sapiens
164 APOBEC3F 200316
Affinity Capture-MS Homo sapiens
165 RPA3 6119
Proximity Label-MS Homo sapiens
166 SIK1  
Affinity Capture-MS Homo sapiens
167 YBX3 8531
Affinity Capture-MS Homo sapiens
168 CDH9 1007
Affinity Capture-MS Homo sapiens
169 FBLL1 345630
Affinity Capture-MS Homo sapiens
170 AP2A2 161
Affinity Capture-MS Homo sapiens
171 RPL35A 6165
Affinity Capture-MS Homo sapiens
172 HSP90AA2P 3324
Affinity Capture-MS Homo sapiens
173 PLCD1 5333
Affinity Capture-MS Homo sapiens
174 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
175 CAPZA2 830
Affinity Capture-MS Homo sapiens
176 GNA14 9630
Affinity Capture-MS Homo sapiens
177 CRYBG3  
Affinity Capture-MS Homo sapiens
178 TCEANC  
Affinity Capture-MS Homo sapiens
179 KIDINS220 57498
Affinity Capture-MS Homo sapiens
180 HEATR1 55127
Affinity Capture-MS Homo sapiens
181 GPR113  
Affinity Capture-MS Homo sapiens
182 PABPC3 5042
Affinity Capture-MS Homo sapiens
183 PAX3  
Affinity Capture-MS Homo sapiens
184 SUPT16H 11198
Affinity Capture-MS Homo sapiens
185 PIP5K1B 8395
Affinity Capture-MS Homo sapiens
186 GNL3 26354
Affinity Capture-MS Homo sapiens
187 NADK2 133686
Affinity Capture-MS Homo sapiens
188 RPS16 6217
Affinity Capture-MS Homo sapiens
189 GDAP2 54834
Affinity Capture-MS Homo sapiens
190 DDX46 9879
Affinity Capture-MS Homo sapiens
191 NES 10763
Affinity Capture-MS Homo sapiens
192 PAXBP1  
Affinity Capture-MS Homo sapiens
193 YBX1 4904
Affinity Capture-MS Homo sapiens
194 PCBP3 54039
Affinity Capture-MS Homo sapiens
195 HMGCR  
Affinity Capture-MS Homo sapiens
196 SSFA2 6744
Affinity Capture-MS Homo sapiens
197 FASN 2194
Affinity Capture-MS Homo sapiens
198 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
199 ARPC5 10092
Affinity Capture-MS Homo sapiens
200 KIAA1671  
Affinity Capture-MS Homo sapiens
201 HIST1H2AC 8334
Affinity Capture-MS Homo sapiens
202 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
203 POLR1B 84172
Affinity Capture-MS Homo sapiens
204 PLEC 5339
Affinity Capture-MS Homo sapiens
205 RRS1 23212
Affinity Capture-MS Homo sapiens
206 PPIP5K1 9677
Affinity Capture-MS Homo sapiens
207 MACF1 23499
Affinity Capture-MS Homo sapiens
208 RBM25 58517
Affinity Capture-MS Homo sapiens
209 RGPD8  
Affinity Capture-MS Homo sapiens
210 RUVBL2 10856
Affinity Capture-MS Homo sapiens
211 PCMT1 5110
Affinity Capture-MS Homo sapiens
212 SRSF9 8683
Affinity Capture-MS Homo sapiens
213 ALPPL2 251
Affinity Capture-MS Homo sapiens
214 MISP 126353
Affinity Capture-MS Homo sapiens
215 SRSF12  
Affinity Capture-MS Homo sapiens
216 PRSS2 5645
Affinity Capture-MS Homo sapiens
217 SH3BP5L  
Affinity Capture-MS Homo sapiens
218 PPOX 5498
Affinity Capture-MS Homo sapiens
219 S100A10 6281
Affinity Capture-MS Homo sapiens
220 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
221 DLK1  
Affinity Capture-MS Homo sapiens
222 G3BP2 9908
Affinity Capture-MS Homo sapiens
223 PUF60 22827
Affinity Capture-MS Homo sapiens
224 TRA2B 6434
Affinity Capture-MS Homo sapiens
225 RANBP2 5903
Affinity Capture-MS Homo sapiens
226 PPL 5493
Affinity Capture-MS Homo sapiens
227 TRIP11 9321
Affinity Capture-MS Homo sapiens
228 RPS15A 6210
Affinity Capture-MS Homo sapiens
229 SSR3 6747
Affinity Capture-MS Homo sapiens
230 SUGP2  
Affinity Capture-MS Homo sapiens
231 ATAD3A 55210
Affinity Capture-MS Homo sapiens
232 AFAP1 60312
Affinity Capture-MS Homo sapiens
233 KRR1 11103
Affinity Capture-MS Homo sapiens
234 MCM5 4174
Affinity Capture-MS Homo sapiens
235 RPS24 6229
Affinity Capture-MS Homo sapiens
236 NCL 4691
Affinity Capture-MS Homo sapiens
237 ALYREF 10189
Affinity Capture-MS Homo sapiens
238 PSMA5 5686
Affinity Capture-MS Homo sapiens
239 SUPT6H 6830
Affinity Capture-MS Homo sapiens
240 TMPRSS13  
Affinity Capture-MS Homo sapiens
241 CEP135  
Affinity Capture-MS Homo sapiens
242 FAM83A  
Affinity Capture-MS Homo sapiens
243 KXD1 79036
Affinity Capture-MS Homo sapiens
244 ITPR1 3708
Affinity Capture-MS Homo sapiens
245 NRIP1 8204
Affinity Capture-MS Homo sapiens
246 PABPC1L 80336
Affinity Capture-MS Homo sapiens
247 RPL23A 6147
Affinity Capture-MS Homo sapiens
248 FAM214B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
249 LOX 4015
Affinity Capture-MS Homo sapiens
250 ANXA2 302
Affinity Capture-MS Homo sapiens
251 MIS18BP1  
Affinity Capture-MS Homo sapiens
252 KLB 152831
Affinity Capture-MS Homo sapiens
253 CEP170 9859
Affinity Capture-MS Homo sapiens
254