Gene description for VTN
Gene name vitronectin
Gene symbol VTN
Other names/aliases V75
VN
VNT
Species Homo sapiens
 Database cross references - VTN
ExoCarta ExoCarta_7448
Vesiclepedia VP_7448
Entrez Gene 7448
HGNC 12724
MIM 193190
UniProt P04004  
 VTN identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 15326289    
 Gene ontology annotations for VTN
Molecular Function
    scavenger receptor activity GO:0005044 IEA
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IDA
    integrin binding GO:0005178 IPI
    extracellular matrix structural constituent GO:0005201 ISS
    extracellular matrix structural constituent GO:0005201 RCA
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IEA
    heparin binding GO:0008201 IEA
    polysaccharide binding GO:0030247 IEA
    identical protein binding GO:0042802 IEA
    extracellular matrix binding GO:0050840 IBA
Biological Process
    immune response GO:0006955 IEA
    cell adhesion GO:0007155 TAS
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 IDA
    cell-matrix adhesion GO:0007160 IGI
    positive regulation of cell-substrate adhesion GO:0010811 IEA
    negative regulation of endopeptidase activity GO:0010951 IDA
    positive regulation of smooth muscle cell migration GO:0014911 IDA
    vesicle-mediated transport GO:0016192 IEA
    cell migration GO:0016477 IGI
    regulation of cell adhesion GO:0030155 IDA
    negative regulation of blood coagulation GO:0030195 IC
    extracellular matrix organization GO:0030198 IEA
    positive regulation of vascular endothelial growth factor receptor signaling pathway GO:0030949 TAS
    positive regulation of protein binding GO:0032092 IDA
    cell adhesion mediated by integrin GO:0033627 IBA
    cell adhesion mediated by integrin GO:0033627 IDA
    endodermal cell differentiation GO:0035987 IDA
    positive regulation of receptor-mediated endocytosis GO:0048260 IDA
    oligodendrocyte differentiation GO:0048709 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    protein polymerization GO:0051258 IEA
    negative regulation of fibrinolysis GO:0051918 NAS
    smooth muscle cell-matrix adhesion GO:0061302 IDA
    positive regulation of wound healing GO:0090303 IC
    liver regeneration GO:0097421 IEA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IEA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum GO:0005783 IDA
    Golgi lumen GO:0005796 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    rough endoplasmic reticulum lumen GO:0048237 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IDA
    collagen-containing extracellular matrix GO:0062023 ISS
    collagen-containing extracellular matrix GO:0062023 NAS
    extracellular exosome GO:0070062 HDA
    alphav-beta3 integrin-vitronectin complex GO:0071062 TAS
    blood microparticle GO:0072562 HDA
    protein complex involved in cell-matrix adhesion GO:0098637 IPI
    peptidase inhibitor complex GO:1904090 IPI
 Experiment description of studies that identified VTN in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
13
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
21
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
22
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
25
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
29
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
30
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
31
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
32
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for VTN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INSIG1  
Affinity Capture-MS Homo sapiens
2 ST6GALNAC4  
Affinity Capture-MS Homo sapiens
3 SLC37A4 2542
Affinity Capture-MS Homo sapiens
4 OTUD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CBX1 10951
Two-hybrid Homo sapiens
6 FBXO33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TRMT61B  
Affinity Capture-MS Homo sapiens
8 VPRBP 9730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RPL13A 23521
Two-hybrid Homo sapiens
10 SF3B1 23451
Co-fractionation Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 HNRNPR 10236
Co-fractionation Homo sapiens
13 POMC  
Reconstituted Complex Homo sapiens
14 SIX1  
Two-hybrid Homo sapiens
15 PRPF40A 55660
Co-fractionation Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 CD247 919
Two-hybrid Homo sapiens
18 ILF2 3608
Co-fractionation Homo sapiens
19 SAFB 6294
Co-fractionation Homo sapiens
20 HOXB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TTLL4  
Affinity Capture-MS Homo sapiens
22 C21orf2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TGFB1 7040
Reconstituted Complex Homo sapiens
24 KIF23 9493
Affinity Capture-MS Homo sapiens
25 USP39 10713
Co-fractionation Homo sapiens
26 TRIOBP 11078
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 COPS5 10987
Two-hybrid Homo sapiens
28 CRADD  
Two-hybrid Homo sapiens
29 ARMCX3 51566
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ISOC1 51015
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 IGF2 3481
Reconstituted Complex Homo sapiens
32 MRS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 GOPC 57120
Two-hybrid Homo sapiens
34 EED  
Affinity Capture-MS Homo sapiens
35 PHF11 51131
Affinity Capture-MS Homo sapiens
36 PRDX4 10549
Two-hybrid Homo sapiens
37 WDR18 57418
Co-fractionation Homo sapiens
38 SNTG1  
Two-hybrid Homo sapiens
39 BUD31 8896
Co-fractionation Homo sapiens
40 SMARCB1 6598
Co-fractionation Homo sapiens
41 RPS11 6205
Co-fractionation Homo sapiens
42 GPRIN1 114787
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KIF14 9928
Affinity Capture-MS Homo sapiens
44 FGF2 2247
Reconstituted Complex Homo sapiens
45 INSIG2  
Affinity Capture-MS Homo sapiens
46 CHMP4C 92421
Affinity Capture-MS Homo sapiens
47 POP1 10940
Affinity Capture-MS Homo sapiens
48 PPP1CA 5499
Co-fractionation Homo sapiens
49 ZNF696  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GNG8  
Affinity Capture-MS Homo sapiens
51 PRPF8 10594
Co-fractionation Homo sapiens
52 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
53 IGFBP5 3488
Reconstituted Complex Homo sapiens
54 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 RAI14 26064
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SMU1 55234
Co-fractionation Homo sapiens
57 ADTRP  
Two-hybrid Homo sapiens
58 GEM  
Two-hybrid Homo sapiens
59 DEK 7913
Co-fractionation Homo sapiens
60 NFIA 4774
Co-fractionation Homo sapiens
61 CHMP4B 128866
Affinity Capture-MS Homo sapiens
62 TRMT11 60487
Affinity Capture-MS Homo sapiens
63 DDX5 1655
Co-fractionation Homo sapiens
64 PTCD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 ARHGAP11A  
Affinity Capture-MS Homo sapiens
66 DLG2 1740
Two-hybrid Homo sapiens
67 SF3B6 51639
Co-fractionation Homo sapiens
68 DHX9 1660
Co-fractionation Homo sapiens
69 TGFB2 7042
Reconstituted Complex Homo sapiens
70 ZNF483  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 BRD4 23476
Affinity Capture-MS Homo sapiens
72 EGF 1950
Reconstituted Complex Homo sapiens
73 SRSF11 9295
Co-fractionation Homo sapiens
74 RBM5  
Affinity Capture-MS Homo sapiens
75 ADH5 128
Two-hybrid Homo sapiens
76 SFPQ 6421
Co-fractionation Homo sapiens
77 RBM39 9584
Co-fractionation Homo sapiens
78 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
79 ACLY 47
Affinity Capture-MS Homo sapiens
80 GLI4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 AGPAT1 10554
Affinity Capture-MS Homo sapiens
82 VEGFA 7422
Reconstituted Complex Homo sapiens
83 RNF14 9604
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 UBQLN2 29978
Two-hybrid Homo sapiens
85 SRSF1 6426
Co-fractionation Homo sapiens
86 WDR33 55339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 RTN2 6253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 NR3C1 2908
Proximity Label-MS Homo sapiens
89 SERPINE1 5054
Reconstituted Complex Homo sapiens
90 ATXN10 25814
Affinity Capture-MS Homo sapiens
91 NAP1L1 4673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 LPCAT3 10162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 UACA 55075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 SF3A1 10291
Co-fractionation Homo sapiens
95 ZNF777  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 RHBDD1 84236
Affinity Capture-MS Homo sapiens
97 PRKACA 5566
Biochemical Activity Homo sapiens
98 CUL9  
Affinity Capture-MS Homo sapiens
99 LACRT 90070
Reconstituted Complex Homo sapiens
100 PRPF19 27339
Co-fractionation Homo sapiens
101 TFDP2  
Affinity Capture-MS Homo sapiens
102 RPP40 10799
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 YWHAQ 10971
Affinity Capture-MS Homo sapiens
104 NHP2L1 4809
Co-fractionation Homo sapiens
105 KNG1 3827
Reconstituted Complex Homo sapiens
106 HP1BP3 50809
Co-fractionation Homo sapiens
107 DDB1 1642
Co-fractionation Homo sapiens
108 SRSF10 10772
Co-fractionation Homo sapiens
109 PVR 5817
Reconstituted Complex Homo sapiens
110 DDRGK1 65992
Affinity Capture-MS Homo sapiens
111 SF3B3 23450
Co-fractionation Homo sapiens
112 SEPT7 989
Co-fractionation Homo sapiens
113 MPRIP 23164
Affinity Capture-MS Homo sapiens
114 HAT1 8520
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 RHOXF1  
Two-hybrid Homo sapiens
116 UBE2U  
Affinity Capture-MS Homo sapiens
117 CCDC8  
Affinity Capture-MS Homo sapiens
118 PHGDH 26227
Co-fractionation Homo sapiens
119 GSG2  
Affinity Capture-MS Homo sapiens
120 DSP 1832
Co-fractionation Homo sapiens
121 KTN1 3895
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 DHX15 1665
Co-fractionation Homo sapiens
123 GLUD1 2746
Two-hybrid Homo sapiens
124 ILF3 3609
Co-fractionation Homo sapiens
125 LNX2  
Two-hybrid Homo sapiens
126 L3MBTL2  
Affinity Capture-MS Homo sapiens
127 RBM25 58517
Co-fractionation Homo sapiens
128 AMPH 273
Reconstituted Complex Homo sapiens
129 ETV7  
Two-hybrid Homo sapiens
130 SAP18 10284
Co-fractionation Homo sapiens
131 RRP1 8568
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 POP4 10775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 HNRNPM 4670
Co-fractionation Homo sapiens
134 SMURF1 57154
Affinity Capture-MS Homo sapiens
135 S100A9 6280
Co-fractionation Homo sapiens
136 NEK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 SLC6A1  
Affinity Capture-MS Homo sapiens
138 UFL1 23376
Affinity Capture-MS Homo sapiens
139 FASTKD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 HACD2 201562
Affinity Capture-MS Homo sapiens
141 MBNL1 4154
Affinity Capture-MS Homo sapiens
142 IK 3550
Co-fractionation Homo sapiens
143 KLK10 5655
Affinity Capture-MS Homo sapiens
144 CDK4 1019
Affinity Capture-MS Homo sapiens
145 PRKCA 5578
Biochemical Activity Homo sapiens
146 CIT 11113
Affinity Capture-MS Homo sapiens
147 C1QBP 708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 CUL7 9820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 HNRNPU 3192
Co-fractionation Homo sapiens
150 CKS2  
Two-hybrid Homo sapiens
151 ITGB5 3693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
152 SNRPA1 6627
Co-fractionation Homo sapiens
153 MATN2 4147
Affinity Capture-MS Homo sapiens
154 CCR1  
Affinity Capture-MS Homo sapiens
155 LUC7L3 51747
Co-fractionation Homo sapiens
156 LEMD2 221496
Affinity Capture-MS Homo sapiens
157 CDC5L 988
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here