Gene description for SF3B1
Gene name splicing factor 3b, subunit 1, 155kDa
Gene symbol SF3B1
Other names/aliases Hsh155
MDS
PRP10
PRPF10
SAP155
SF3b155
Species Homo sapiens
 Database cross references - SF3B1
ExoCarta ExoCarta_23451
Entrez Gene 23451
HGNC 10768
MIM 605590
UniProt O75533  
 SF3B1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SF3B1
Molecular Function
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 TAS
    RNA splicing GO:0008380 TAS
    RNA splicing, via transesterification reactions GO:0000375 NAS
    anterior/posterior pattern specification GO:0009952 IEA
    gene expression GO:0010467 TAS
Subcellular Localization
    spliceosomal complex GO:0005681 NAS
    nuclear speck GO:0016607 IEA
    catalytic step 2 spliceosome GO:0071013 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    U12-type spliceosomal complex GO:0005689 IDA
 Experiment description of studies that identified SF3B1 in exosomes
1
Experiment ID 282
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SF3B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCNE1  
Affinity Capture-Western Homo sapiens
2 BRF2  
Affinity Capture-MS Homo sapiens
3 SF3B6 51639
Reconstituted Complex Homo sapiens
4 SF3B3 23450
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 TCERG1  
Affinity Capture-MS Homo sapiens
6 SF3B2 10992
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 HDAC3 8841
Affinity Capture-MS Homo sapiens
8 SMNDC1  
Affinity Capture-MS Homo sapiens
9 RNPS1 10921
Affinity Capture-MS Homo sapiens
10 RNU12  
Affinity Capture-MS Homo sapiens
11 APG12L  
Affinity Capture-MS Homo sapiens
12 RNU11 26824
Affinity Capture-MS Homo sapiens
13 PPP1R8 5511
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
14 GSK3B 2932
Affinity Capture-MS Homo sapiens
15 PUF60 22827
Affinity Capture-MS Homo sapiens
16 YWHAZ 7534
Affinity Capture-MS Homo sapiens
17 SNRNP40 9410
Affinity Capture-MS Homo sapiens
18 PHF5A 84844
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which SF3B1 is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome





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