Experiment description of studies that identified RNU12 in exosomes

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule snRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 222 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule snRNA Identification method RT-PCR PubMed ID 23663360     Organism Homo sapiens Experiment description Characterization of human plasma-derived exosomal RNAs by deep sequencing Authors "Huang X, Yuan T, Tschannen M, Sun Z, Jacob H, Du M, Liang M, Dittmar RL, Liu Y, Liang M, Kohli M, Thibodeau SN, Boardman L, Wang L" Journal name BMC Genomics Publication year 2013 Sample Plasma Sample name Plasma - Normal Isolation/purification methods Exoquick Flotation density - Molecules identified in the study miRNA tRNA snRNA snoRNA rRNA piRNA lncRNA Methods used in the study RT-PCR

Protein-protein interactions for RNU12

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YBX1 4904 Affinity Capture-MS Homo sapiens 2 SF3B2 10992 Affinity Capture-MS Homo sapiens 3 SNRPG 6637 Affinity Capture-MS Homo sapiens 4 SNRNP35   Affinity Capture-MS Homo sapiens 5 DHX15 1665 Affinity Capture-MS Homo sapiens 6 SF3B4 10262 Affinity Capture-MS Homo sapiens 7 PRPF6 24148 Affinity Capture-MS Homo sapiens 8 ZCRB1   Affinity Capture-MS Homo sapiens 9 PRPF8 10594 Affinity Capture-MS Homo sapiens 10 G3BP1 10146 Affinity Capture-RNA Homo sapiens 11 ZMAT5   Affinity Capture-MS Homo sapiens 12 SNRNP25   Affinity Capture-MS Homo sapiens 13 UBAP2L 9898 Affinity Capture-RNA Homo sapiens 14 RNPC3   Affinity Capture-MS Homo sapiens 15 EFTUD2 9343 Affinity Capture-MS Homo sapiens 16 SNRNP200 23020 Affinity Capture-MS Homo sapiens 17 SNRPD1 6632 Affinity Capture-MS Homo sapiens 18 SF3B1 23451 Affinity Capture-MS Homo sapiens 19 PHF5A 84844 Affinity Capture-MS Homo sapiens 20 SF3B3 23450 Affinity Capture-MS Homo sapiens 21 SNRNP48   Affinity Capture-MS Homo sapiens 22 PDCD7   Affinity Capture-MS Homo sapiens 23 SNRPD2 6633 Affinity Capture-MS Homo sapiens 24 SNRPD3 6634 Affinity Capture-MS Homo sapiens 25 U2AF1 7307 Affinity Capture-MS Homo sapiens View the network

Pathways in which RNU12 is involved

No pathways found