Gene description for RNU12
Gene name RNA, U12 small nuclear
Gene symbol RNU12
Other names/aliases RNU12-1
RNU12L
RNU12P
dJ222E13.7
Species Homo sapiens
 Database cross references - RNU12
ExoCarta ExoCarta_267010
Entrez Gene 267010
HGNC 19380
 RNU12 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Plasma 23663360    
 Gene ontology annotations for RNU12
 Experiment description of studies that identified RNU12 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule snRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 222
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule snRNA
Identification method RT-PCR
PubMed ID 23663360    
Organism Homo sapiens
Experiment description Characterization of human plasma-derived exosomal RNAs by deep sequencing
Authors Huang X, Yuan T, Tschannen M, Sun Z, Jacob H, Du M, Liang M, Dittmar RL, Liu Y, Liang M, Kohli M, Thibodeau SN, Boardman L, Wang L
Journal name BMC Genomics
Publication year 2013
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Exoquick
Flotation density -
Molecules identified in the study miRNA
tRNA
snRNA
snoRNA
rRNA
piRNA
lncRNA
Methods used in the study RT-PCR
 Protein-protein interactions for RNU12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RNU12 is involved
No pathways found





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