|
 Gene description for RNU12 |
| Gene name |
RNA, U12 small nuclear |
| Gene symbol |
RNU12 |
| Other names/aliases |
RNU12-1
RNU12L
RNU12P
dJ222E13.7 |
| Species |
Homo sapiens |
| Database cross references - RNU12 |
| ExoCarta |
ExoCarta_267010 |
| Entrez Gene |
267010 |
| HGNC |
19380 |
| RNU12 identified in exosomes derived from the following tissue/cell type |
|
Endothelial cells
|
26027894
|
|
Plasma
|
23663360
|
| Gene ontology annotations for RNU12 |
|
|
| Experiment description of studies that identified RNU12 in exosomes |
| 1 |
| Experiment ID |
226 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
GAPDH
|
EV Cytosolic markers |
|
✔
CD9|FLOT1
|
EV Membrane markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
snRNA
|
| Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
| Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting |
|
|
| 2 |
| Experiment ID |
222 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
snRNA
|
| Identification method |
RT-PCR
|
| PubMed ID |
23663360
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of human plasma-derived exosomal RNAs by deep sequencing |
| Authors |
Huang X, Yuan T, Tschannen M, Sun Z, Jacob H, Du M, Liang M, Dittmar RL, Liu Y, Liang M, Kohli M, Thibodeau SN, Boardman L, Wang L |
| Journal name |
BMC Genomics
|
| Publication year |
2013 |
| Sample |
Plasma |
| Sample name |
Plasma - Normal |
| Isolation/purification methods |
Exoquick |
| Flotation density |
-
|
| Molecules identified in the study |
miRNA
tRNA
snRNA
snoRNA
rRNA
piRNA
lncRNA |
| Methods used in the study |
RT-PCR |
|
|
| Protein-protein interactions for RNU12 |
| |
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
|
No interactions are found.
|
|
| Pathways in which RNU12 is involved |
|
No pathways found
|
|
|
