Gene description for SNRPD1
Gene name small nuclear ribonucleoprotein D1 polypeptide 16kDa
Gene symbol SNRPD1
Other names/aliases HsT2456
SMD1
SNRPD
Sm-D1
Species Homo sapiens
 Database cross references - SNRPD1
ExoCarta ExoCarta_6632
Entrez Gene 6632
HGNC 11158
MIM 601063
UniProt P62314  
 SNRPD1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Urine 15326289    
 Gene ontology annotations for SNRPD1
Molecular Function
    RNA binding GO:0003723 TAS
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 TAS
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    ncRNA metabolic process GO:0034660 TAS
    gene expression GO:0010467 TAS
    spliceosomal complex assembly GO:0000245 TAS
    RNA splicing GO:0008380 TAS
    spliceosomal snRNP assembly GO:0000387 TAS
    positive regulation of defense response to virus by host GO:0002230 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    methylosome GO:0034709 IDA
    SMN-Sm protein complex GO:0034719 IDA
    nucleoplasm GO:0005654 TAS
    U12-type spliceosomal complex GO:0005689 IDA
    small nuclear ribonucleoprotein complex GO:0030532 TAS
    catalytic step 2 spliceosome GO:0071013 IDA
    U4 snRNP GO:0005687 IDA
    pICln-Sm protein complex GO:0034715 IDA
    nucleus GO:0005634 IMP
    U1 snRNP GO:0005685 IDA
 Experiment description of studies that identified SNRPD1 in exosomes
1
Experiment ID 285
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 286
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 255
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for SNRPD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LSM10  
Affinity Capture-MS Homo sapiens
2 RNU11 26824
Affinity Capture-MS Homo sapiens
3 GEMIN5 25929
Reconstituted Complex Homo sapiens
4 LSM8  
Two-hybrid Homo sapiens
5 PRMT5 10419
Reconstituted Complex Homo sapiens
6 RNU12  
Affinity Capture-MS Homo sapiens
7 SMN1 6606
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
8 WDR77 79084
Reconstituted Complex Homo sapiens
9 DDX20  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 CLNS1A 1207
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 SNRPD2 6633
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SNRPD1 is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
snRNP Assembly TAS Reactome





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