Gene description for VRK3
Gene name vaccinia related kinase 3
Gene symbol VRK3
Other names/aliases -
Species Homo sapiens
 Database cross references - VRK3
ExoCarta ExoCarta_51231
Entrez Gene 51231
HGNC 18996
UniProt Q8IV63  
 VRK3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for VRK3
Molecular Function
    protein phosphatase binding GO:0019903 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    ATP binding GO:0005524 IEA
Biological Process
    regulation of cell shape GO:0008360 IBA
    positive regulation of phosphoprotein phosphatase activity GO:0032516 IEA
    peptidyl-serine phosphorylation GO:0018105 IBA
    endocytosis GO:0006897 IBA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    Wnt signaling pathway GO:0016055 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified VRK3 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for VRK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TWF2 11344
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which VRK3 is involved
No pathways found





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