Experiment description of studies that identified USP54 in exosomes

1 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for USP54

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CHMP2A 27243 Reconstituted Complex Homo sapiens 2 RBMXL1   Affinity Capture-MS Homo sapiens 3 NXT2   Two-hybrid Homo sapiens 4 KRT2 3849 Affinity Capture-MS Homo sapiens 5 HOOK1   Proximity Label-MS Homo sapiens 6 EPM2AIP1 9852 Two-hybrid Homo sapiens 7 LGR4 55366 Affinity Capture-MS Homo sapiens 8 CCDC24   Two-hybrid Homo sapiens 9 OCLN 100506658 Proximity Label-MS Homo sapiens 10 ATXN7L2   Two-hybrid Homo sapiens 11 AES 166 Two-hybrid Homo sapiens 12 BBS7 55212 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 PARD3 56288 Proximity Label-MS Homo sapiens 14 P4HA2 8974 Affinity Capture-MS Homo sapiens 15 CRYL1 51084 Affinity Capture-MS Homo sapiens 16 TRIM66   Affinity Capture-MS Homo sapiens 17 CELA1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 KIF20A 10112 Affinity Capture-MS Homo sapiens 19 UBC 7316 Reconstituted Complex Homo sapiens 20 CSNK2A1 1457 Affinity Capture-MS Homo sapiens 21 BUD13   Affinity Capture-MS Homo sapiens 22 ATN1   Two-hybrid Homo sapiens 23 THSD4 79875 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 EIF4E2   Two-hybrid Homo sapiens 25 ARMC7   Two-hybrid Homo sapiens 26 LGALS3BP 3959 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 PRR35   Two-hybrid Homo sapiens 28 MLLT4 4301 Proximity Label-MS Homo sapiens 29 FBXO17 115290 Two-hybrid Homo sapiens 30 DYRK1A 1859 Affinity Capture-MS Homo sapiens 31 PLAC8   Two-hybrid Homo sapiens 32 CARD8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 TBX6   Two-hybrid Homo sapiens 34 ZNF148   Two-hybrid Homo sapiens 35 HGS 9146 Two-hybrid Homo sapiens 36 MLST8 64223 Affinity Capture-MS Homo sapiens 37 OIP5   Two-hybrid Homo sapiens 38 TSEN15 116461 Two-hybrid Homo sapiens 39 CHMP2B 25978 Reconstituted Complex Homo sapiens 40 CHMP1B 57132 Reconstituted Complex Homo sapiens 41 YWHAH 7533 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 IGFN1   Two-hybrid Homo sapiens 43 IL17RA 23765 Affinity Capture-MS Homo sapiens 44 YWHAG 7532 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 YWHAB 7529 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 CCT2 10576 Affinity Capture-MS Homo sapiens 47 CPEB1   Proximity Label-MS Homo sapiens 48 CHMP4C 92421 Reconstituted Complex Homo sapiens 49 VRK3 51231 Affinity Capture-MS Homo sapiens 50 S100A2 6273 Affinity Capture-MS Homo sapiens 51 CCDC13 152206 Two-hybrid Homo sapiens 52 TEKT5   Two-hybrid Homo sapiens 53 PRPH 5630 Proximity Label-MS Homo sapiens 54 NCKIPSD 51517 Proximity Label-MS Homo sapiens 55 CXADR 1525 Proximity Label-MS Homo sapiens 56 CSTF2 1478 Two-hybrid Homo sapiens 57 TCEA2   Two-hybrid Homo sapiens 58 KIAA1683   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 TSSK3   Two-hybrid Homo sapiens 60 CYSRT1 375791 Two-hybrid Homo sapiens 61 PIGT 51604 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 62 PNMA2   Affinity Capture-MS Homo sapiens 63 CLEC11A 6320 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 64 BICD2 23299 Proximity Label-MS Homo sapiens 65 MED25   Two-hybrid Homo sapiens 66 VENTX   Two-hybrid Homo sapiens 67 FAM124B   Two-hybrid Homo sapiens 68 SS18L1   Two-hybrid Homo sapiens 69 LATS2 26524 Proximity Label-MS Homo sapiens 70 YWHAE 7531 Affinity Capture-MS Homo sapiens 71 ATXN1 6310 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens 72 DISC1 27185 Affinity Capture-MS Homo sapiens 73 HABP4   Affinity Capture-MS Homo sapiens 74 NUDT22   Two-hybrid Homo sapiens 75 C15orf41   Affinity Capture-MS Homo sapiens 76 KCTD9   Two-hybrid Homo sapiens 77 SNRPB 6628 Two-hybrid Homo sapiens 78 CTNNB1 1499 Proximity Label-MS Homo sapiens 79 FOXI1   Two-hybrid Homo sapiens 80 PRSS50   Affinity Capture-MS Homo sapiens 81 YWHAZ 7534 Affinity Capture-MS Homo sapiens 82 FAM46B   Two-hybrid Homo sapiens 83 PHF1   Two-hybrid Homo sapiens 84 BHLHE40   Two-hybrid Homo sapiens 85 BFSP2   Two-hybrid Homo sapiens 86 CCNJL   Affinity Capture-MS Homo sapiens 87 HNRNPF 3185 Two-hybrid Homo sapiens 88 OTX1   Two-hybrid Homo sapiens 89 SEC24A 10802 Two-hybrid Homo sapiens 90 RP9   Affinity Capture-MS Homo sapiens 91 ABTB2 25841 Affinity Capture-MS Homo sapiens 92 CHCHD2   Two-hybrid Homo sapiens 93 PRPF38A 84950 Affinity Capture-MS Homo sapiens 94 NIN 51199 Proximity Label-MS Homo sapiens 95 TNS2 23371 Two-hybrid Homo sapiens 96 BICD1 636 Proximity Label-MS Homo sapiens 97 FLII 2314 Affinity Capture-MS Homo sapiens 98 GCM2   Two-hybrid Homo sapiens 99 SHC3   Two-hybrid Homo sapiens 100 PLA2G6 8398 Two-hybrid Homo sapiens 101 PRKAB2 5565 Two-hybrid Homo sapiens 102 RNF208   Two-hybrid Homo sapiens 103 KRT19 3880 Proximity Label-MS Homo sapiens 104 SMYD1   Two-hybrid Homo sapiens 105 STIL   Proximity Label-MS Homo sapiens 106 FLOT1 10211 Proximity Label-MS Homo sapiens 107 FAM103A1   Two-hybrid Homo sapiens 108 ZCCHC14 23174 Two-hybrid Homo sapiens 109 INSC   Two-hybrid Homo sapiens 110 RNF43   Proximity Label-MS Homo sapiens 111 ASB3 51130 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 112 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 113 FBXW11   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 114 STK16 8576 Two-hybrid Homo sapiens 115 SIRT6   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 116 PDCL3 79031 Affinity Capture-MS Homo sapiens 117 RHOBTB3 22836 Two-hybrid Homo sapiens 118 EXOC8 149371 Two-hybrid Homo sapiens 119 ZEB1   Affinity Capture-MS Homo sapiens 120 ATXN1L   Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens 121 DOK6   Two-hybrid Homo sapiens 122 POLR1C 9533 Two-hybrid Homo sapiens 123 LCK 3932 Proximity Label-MS Homo sapiens 124 NINL   Proximity Label-MS Homo sapiens 125 SQSTM1 8878 Proximity Label-MS Homo sapiens 126 PDDC1 347862 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 127 OLFM4 10562 Affinity Capture-MS Homo sapiens 128 LATS1   Proximity Label-MS Homo sapiens 129 ANAPC2 29882 Proximity Label-MS Homo sapiens 130 TRIM52   Affinity Capture-MS Homo sapiens 131 SKAP1 8631 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 132 PPP4R1L   Affinity Capture-MS Homo sapiens 133 SNIP1   Affinity Capture-MS Homo sapiens 134 QRICH1   Two-hybrid Homo sapiens 135 CEP135   Proximity Label-MS Homo sapiens 136 CDC16 8881 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 137 RBPMS2   Two-hybrid Homo sapiens 138 OLFM2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 139 ANKRD11 29123 Two-hybrid Homo sapiens 140 SHARPIN   Two-hybrid Homo sapiens 141 HSD17B7P2   Affinity Capture-MS Homo sapiens 142 HNF1B   Two-hybrid Homo sapiens 143 TAGLN 6876 Affinity Capture-MS Homo sapiens 144 GNB2L1 10399 Two-hybrid Homo sapiens 145 AR 367 Affinity Capture-MS Homo sapiens 146 VPS39 23339 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 147 CCDC96   Affinity Capture-MS Homo sapiens 148 PLEKHN1   Two-hybrid Homo sapiens 149 CAPN2 824 Affinity Capture-MS Homo sapiens View the network

Pathways in which USP54 is involved

No pathways found