Gene description for ANAPC2
Gene name anaphase promoting complex subunit 2
Gene symbol ANAPC2
Other names/aliases APC2
Species Homo sapiens
 Database cross references - ANAPC2
ExoCarta ExoCarta_29882
Entrez Gene 29882
HGNC 19989
MIM 606946
UniProt Q9UJX6  
 ANAPC2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for ANAPC2
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
Biological Process
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    positive regulation of dendrite morphogenesis GO:0050775 IEA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    mitotic spindle assembly checkpoint GO:0007094 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    mitotic cell cycle GO:0000278 TAS
    positive regulation of synaptic plasticity GO:0031915 ISS
    mitotic nuclear division GO:0007067 IEA
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    cell division GO:0051301 IEA
    positive regulation of axon extension GO:0045773 IEA
    protein K11-linked ubiquitination GO:0070979 IDA
    positive regulation of synapse maturation GO:0090129 ISS
Subcellular Localization
    anaphase-promoting complex GO:0005680 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified ANAPC2 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ANAPC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC4  
Reconstituted Complex Homo sapiens
2 ANAPC1 64682
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
3 ANAPC11  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 ANAPC5 51433
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
5 UBE2C  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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