Gene description for PIK3C2A
Gene name phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha
Gene symbol PIK3C2A
Other names/aliases CPK
PI3-K-C2(ALPHA)
PI3-K-C2A
Species Homo sapiens
 Database cross references - PIK3C2A
ExoCarta ExoCarta_5286
Vesiclepedia VP_5286
Entrez Gene 5286
HGNC 8971
MIM 603601
UniProt O00443  
 PIK3C2A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for PIK3C2A
Molecular Function
    ATP binding GO:0005524 IEA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IBA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IDA
    1-phosphatidylinositol-3-kinase activity GO:0016303 TAS
    clathrin binding GO:0030276 IDA
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 IBA
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 IDA
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 TAS
    phosphatidylinositol binding GO:0035091 IEA
    1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity GO:0046934 IDA
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    exocytosis GO:0006887 TAS
    endocytosis GO:0006897 IMP
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    positive regulation of autophagy GO:0010508 IMP
    vascular associated smooth muscle contraction GO:0014829 TAS
    cell migration GO:0016477 IBA
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IBA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IBA
    platelet-derived growth factor receptor signaling pathway GO:0048008 TAS
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    clathrin coat assembly GO:0048268 TAS
    membrane organization GO:0061024 TAS
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    clathrin-coated vesicle GO:0030136 IDA
    vesicle GO:0031982 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PIK3C2A in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PIK3C2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 RPA2 6118
Proximity Label-MS Homo sapiens
3 AP2B1 163
Proximity Label-MS Homo sapiens
4 PPP1CB 5500
Affinity Capture-MS Homo sapiens
5 LGR4 55366
Affinity Capture-MS Homo sapiens
6 PMS1  
Affinity Capture-Western Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 POGZ 23126
Affinity Capture-MS Homo sapiens
9 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 Calml3  
Affinity Capture-MS Mus musculus
11 DBN1 1627
Affinity Capture-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 FKBP4 2288
Affinity Capture-MS Homo sapiens
14 Actb 11461
Affinity Capture-MS Mus musculus
15 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
17 CAPZB 832
Affinity Capture-MS Homo sapiens
18 PARK2  
Affinity Capture-MS Homo sapiens
19 CDC16 8881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ALDH1A1 216
Affinity Capture-MS Homo sapiens
21 YWHAG 7532
Affinity Capture-MS Homo sapiens
22 IQGAP1 8826
Affinity Capture-MS Homo sapiens
23 GUSBP5  
Affinity Capture-MS Homo sapiens
24 MAGED2 10916
Affinity Capture-MS Homo sapiens
25 FAM136A 84908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 STX6 10228
Proximity Label-MS Homo sapiens
27 ATG7 10533
Affinity Capture-MS Homo sapiens
28 DNAJC7 7266
Proximity Label-MS Homo sapiens
29 PMS2  
Affinity Capture-MS Homo sapiens
30 GTSE1 51512
Affinity Capture-MS Homo sapiens
31 SEC23A 10484
Affinity Capture-MS Homo sapiens
32 OASL 8638
Affinity Capture-MS Homo sapiens
33 TMEM74  
Affinity Capture-MS Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 METTL10  
Affinity Capture-MS Homo sapiens
36 HSPA8 3312
Affinity Capture-MS Homo sapiens
37 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 POLR2K  
Affinity Capture-MS Homo sapiens
39 NUP155 9631
Proximity Label-MS Homo sapiens
40 PFN1 5216
Affinity Capture-MS Homo sapiens
41 MYO18A 399687
Affinity Capture-MS Homo sapiens
42 QPRT 23475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 Sec24c  
Affinity Capture-MS Mus musculus
44 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 YWHAE 7531
Affinity Capture-MS Homo sapiens
46 MYH9 4627
Affinity Capture-MS Homo sapiens
47 LRCH1 23143
Affinity Capture-MS Homo sapiens
48 ACTN4 81
Affinity Capture-MS Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
50 PILRA 29992
Affinity Capture-MS Homo sapiens
51 GPR182  
Affinity Capture-MS Homo sapiens
52 THBS3 7059
Affinity Capture-MS Homo sapiens
53 SLC7A6 9057
Affinity Capture-MS Homo sapiens
54 DHFRL1  
Proximity Label-MS Homo sapiens
55 C10orf88  
Affinity Capture-MS Homo sapiens
56 RPLP0 6175
Co-fractionation Homo sapiens
57 STAMBP 10617
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 LYPD4  
Affinity Capture-MS Homo sapiens
59 GAK 2580
Affinity Capture-MS Homo sapiens
60 RMDN3 55177
Proximity Label-MS Homo sapiens
61 YWHAH 7533
Affinity Capture-MS Homo sapiens
62 ANLN 54443
Affinity Capture-MS Homo sapiens
63 LIMA1 51474
Affinity Capture-MS Homo sapiens
64 BACE2 25825
Affinity Capture-MS Homo sapiens
65 CLINT1 9685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 BAG2 9532
Affinity Capture-MS Homo sapiens
67 RBM47 54502
Affinity Capture-MS Homo sapiens
68 SNAPC4  
Affinity Capture-MS Homo sapiens
69 NCAPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 YWHAZ 7534
Affinity Capture-MS Homo sapiens
71 MYO19  
Affinity Capture-MS Homo sapiens
72 CCNJL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 DUSP16  
Affinity Capture-MS Homo sapiens
74 SLC25A41  
Affinity Capture-MS Homo sapiens
75 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
76 AKR1C2  
Affinity Capture-MS Homo sapiens
77 FKBP5 2289
Affinity Capture-MS Homo sapiens
78 SYNPO 11346
Affinity Capture-MS Homo sapiens
79 ANKFY1 51479
Affinity Capture-MS Homo sapiens
80 MYO6 4646
Affinity Capture-MS Homo sapiens
81 Uso1 56041
Affinity Capture-MS Mus musculus
82 SEC16A 9919
Affinity Capture-MS Homo sapiens
83 METTL21B  
Affinity Capture-MS Homo sapiens
84 PTPN23 25930
Proximity Label-MS Homo sapiens
85 DUSP21  
Affinity Capture-MS Homo sapiens
86 TRIM28 10155
Affinity Capture-MS Homo sapiens
87 CLTC 1213
Affinity Capture-MS Homo sapiens
88 STAMBPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 MAP7D2 256714
Affinity Capture-MS Homo sapiens
90 AKAP1 8165
Proximity Label-MS Homo sapiens
91 DNAJC6  
Affinity Capture-MS Homo sapiens
92 PA2G4 5036
Affinity Capture-MS Homo sapiens
93 TMSB10 9168
Affinity Capture-MS Homo sapiens
94 ARRB2 409
Affinity Capture-MS Homo sapiens
95 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
96 FBXW11  
Affinity Capture-MS Homo sapiens
97 STK24 8428
Affinity Capture-MS Homo sapiens
98 LAMTOR1 55004
Proximity Label-MS Homo sapiens
99 PGK1 5230
Affinity Capture-MS Homo sapiens
100 DNAJA2 10294
Proximity Label-MS Homo sapiens
101 TRIM27  
Affinity Capture-Western Homo sapiens
102 Myo1c 17913
Affinity Capture-MS Mus musculus
103 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
104 EIF5A2 56648
Affinity Capture-MS Homo sapiens
105 CAPZA2 830
Affinity Capture-MS Homo sapiens
106 SQSTM1 8878
Proximity Label-MS Homo sapiens
107 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
108 MLH1 4292
Affinity Capture-MS Homo sapiens
109 TKT 7086
Affinity Capture-MS Homo sapiens
110 PICALM 8301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 NUP35 129401
Proximity Label-MS Homo sapiens
112 MAGEA9  
Affinity Capture-MS Homo sapiens
113 DDX58 23586
Affinity Capture-RNA Homo sapiens
114 TARS 6897
Affinity Capture-MS Homo sapiens
115 Myh10 77579
Affinity Capture-MS Mus musculus
116 ANAPC2 29882
Proximity Label-MS Homo sapiens
117 C6orf141  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 PPP4R1L  
Affinity Capture-MS Homo sapiens
119 TRIM37  
Proximity Label-MS Homo sapiens
120 NQO1 1728
Affinity Capture-MS Homo sapiens
121 ARRB1 408
Affinity Capture-MS Homo sapiens
122 FLNA 2316
Affinity Capture-MS Homo sapiens
123 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
124 POLR3B 55703
Affinity Capture-MS Homo sapiens
125 PTGES3 10728
Affinity Capture-MS Homo sapiens
126 CCDC96  
Affinity Capture-MS Homo sapiens
127 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 Sept9  
Affinity Capture-MS Mus musculus
129 RPA3 6119
Proximity Label-MS Homo sapiens
130 ARX  
Affinity Capture-MS Homo sapiens
131 CXCL16 58191
Affinity Capture-MS Homo sapiens
132 CAPN2 824
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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