Gene description for PIK3C2A
Gene name phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha
Gene symbol PIK3C2A
Other names/aliases CPK
PI3-K-C2(ALPHA)
PI3-K-C2A
Species Homo sapiens
 Database cross references - PIK3C2A
ExoCarta ExoCarta_5286
Entrez Gene 5286
HGNC 8971
MIM 603601
UniProt O00443  
 PIK3C2A identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for PIK3C2A
Molecular Function
    phosphatidylinositol 3-kinase activity GO:0035004 TAS
    phosphatidylinositol binding GO:0035091 IEA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IEA
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 TAS
    ATP binding GO:0005524 IEA
Biological Process
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IBA
    vascular smooth muscle contraction GO:0014829 TAS
    platelet-derived growth factor receptor signaling pathway GO:0048008 TAS
    exocytosis GO:0006887 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    innate immune response GO:0045087 IBA
    endocytosis GO:0006897 IMP
    small molecule metabolic process GO:0044281 TAS
    inflammatory response GO:0006954 IBA
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    platelet activation GO:0030168 IBA
    clathrin coat assembly GO:0048268 TAS
    phospholipid metabolic process GO:0006644 TAS
    protein phosphorylation GO:0006468 IBA
    cell chemotaxis GO:0060326 IBA
    adaptive immune response GO:0002250 IBA
    phosphatidylinositol phosphorylation GO:0046854 IBA
    negative regulation of zinc ion transmembrane transport GO:0071583 IEA
    phosphatidylinositol 3-kinase signaling GO:0014065 IBA
    insulin receptor signaling pathway GO:0008286 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    phosphatidylinositol 3-kinase complex GO:0005942 IBA
    nucleus GO:0005634 IEA
    vesicle GO:0031982 IDA
    clathrin-coated vesicle GO:0030136 IEA
    plasma membrane GO:0005886 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    Golgi apparatus GO:0005794 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified PIK3C2A in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PIK3C2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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