Gene description for MLH1
Gene name mutL homolog 1
Gene symbol MLH1
Other names/aliases COCA2
FCC2
HNPCC
HNPCC2
hMLH1
Species Homo sapiens
 Database cross references - MLH1
ExoCarta ExoCarta_4292
Vesiclepedia VP_4292
Entrez Gene 4292
HGNC 7127
MIM 120436
UniProt P40692  
 MLH1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Thymus 23844026    
 Gene ontology annotations for MLH1
Molecular Function
    ATP binding GO:0005524 IEA
    guanine/thymine mispair binding GO:0032137 IEA
    protein binding GO:0005515 IPI
    MutSalpha complex binding GO:0032407 IDA
    single-stranded DNA binding GO:0003697 IDA
    ATPase activity GO:0016887 IBA
Biological Process
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IEA
    somatic hypermutation of immunoglobulin genes GO:0016446 IBA
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 IEA
    mismatch repair GO:0006298 TAS
    double-strand break repair via nonhomologous end joining GO:0006303 IEA
    meiotic metaphase I plate congression GO:0043060 IEA
    resolution of meiotic recombination intermediates GO:0000712 IEA
    DNA repair GO:0006281 TAS
    negative regulation of mitotic recombination GO:0045950 IEA
    isotype switching GO:0045190 IEA
    spermatogenesis GO:0007283 IEA
    oogenesis GO:0048477 IEA
    meiotic spindle midzone assembly GO:0051257 IEA
    synapsis GO:0007129 IEA
    male meiosis chromosome segregation GO:0007060 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    MutLalpha complex GO:0032389 IBA
    male germ cell nucleus GO:0001673 IEA
    membrane GO:0016020 IDA
    chiasma GO:0005712 IBA
    nucleus GO:0005634 IC
    synaptonemal complex GO:0000795 IBA
 Experiment description of studies that identified MLH1 in exosomes
1
Experiment ID 237
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 217
MISEV standards
EM
EV Biophysical techniques
TSG101|CD81|CD9|CD63
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MLH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLM 641
Two-hybrid Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
2 NBN 4683
Co-purification Homo sapiens
3 PMS2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 MSH4  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 MLH3  
Two-hybrid Homo sapiens
6 ZC3HDC11A  
Two-hybrid Homo sapiens
7 BRCA1 672
Co-purification Homo sapiens
8 AP2B1 163
Two-hybrid Homo sapiens
9 RAD50 10111
Co-purification Homo sapiens
10 TRIM29 23650
Two-hybrid Homo sapiens
11 ATM 472
Co-purification Homo sapiens
12 MYC  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
13 MRE11A 4361
Co-purification Homo sapiens
14 RFC1 5981
Co-purification Homo sapiens
15 PMS1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 MSH6 2956
Co-purification Homo sapiens
17 MSH2 4436
Co-purification Homo sapiens
18 FRMD6 122786
Two-hybrid Homo sapiens
19 MBD4  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
20 UBOX5  
Two-hybrid Homo sapiens
21 EXO1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
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