Gene description for CASP3
Gene name caspase 3, apoptosis-related cysteine peptidase
Gene symbol CASP3
Other names/aliases CPP32
CPP32B
SCA-1
Species Homo sapiens
 Database cross references - CASP3
ExoCarta ExoCarta_836
Vesiclepedia VP_836
Entrez Gene 836
HGNC 1504
MIM 600636
UniProt P42574  
 CASP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for CASP3
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 TAS
    peptidase activity GO:0008233 IDA
    cysteine-type endopeptidase activity involved in apoptotic process GO:0097153 IMP
    protein binding GO:0005515 IPI
    cyclin-dependent protein serine/threonine kinase inhibitor activity GO:0004861 IEA
    cysteine-type endopeptidase activity involved in execution phase of apoptosis GO:0097200 IMP
    aspartic-type endopeptidase activity GO:0004190 EXP
Biological Process
    cellular response to DNA damage stimulus GO:0006974 IEA
    heart development GO:0007507 IEA
    extracellular matrix disassembly GO:0022617 TAS
    regulation of apoptotic DNA fragmentation GO:1902510 IEA
    release of cytochrome c from mitochondria GO:0001836 IEA
    erythrocyte differentiation GO:0030218 TAS
    T cell homeostasis GO:0043029 IEA
    neuron apoptotic process GO:0051402 IEA
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    apoptotic DNA fragmentation GO:0006309 TAS
    cell fate commitment GO:0045165 IEA
    apoptotic process GO:0006915 TAS
    response to wounding GO:0009611 IEA
    execution phase of apoptosis GO:0097194 IMP
    platelet formation GO:0030220 TAS
    proteolysis GO:0006508 IDA
    response to UV GO:0009411 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c GO:0008635 TAS
    hippo signaling GO:0035329 TAS
    response to tumor necrosis factor GO:0034612 TAS
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IEA
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    negative regulation of activated T cell proliferation GO:0046007 IEA
    keratinocyte differentiation GO:0030216 IBA
    neuron differentiation GO:0030182 IBA
    regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043281 TAS
    sensory perception of sound GO:0007605 IEA
    negative regulation of apoptotic process GO:0043066 IGI
    extracellular matrix organization GO:0030198 TAS
    glial cell apoptotic process GO:0034349 IEA
    apoptotic signaling pathway GO:0097190 TAS
    B cell homeostasis GO:0001782 IEA
    programmed cell death GO:0012501 TAS
    negative regulation of B cell proliferation GO:0030889 IEA
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IEA
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    negative regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045736 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cytoplasm GO:0005737 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CASP3 in exosomes
1
Experiment ID 489
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CASP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT18 3875
Affinity Capture-MS Homo sapiens
2 HSPE1 3336
Invitro Homo sapiens
3 BIRC2  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
4 PPP3CA 5530
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 PARG 8505
Biochemical Activity Homo sapiens
6 XIAP  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
7 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 ATXN3 4287
Affinity Capture-MS Homo sapiens
9 NDUFS1 4719
Affinity Capture-MS Homo sapiens
10 CSEN  
Affinity Capture-MS Homo sapiens
11 LMNB1 4001
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
12 YWHAE 7531
Affinity Capture-MS Homo sapiens
13 CTNNB1 1499
Invivo Homo sapiens
14 DFFA 1676
Affinity Capture-MS Homo sapiens
15 CASP2  
Biochemical Activity Homo sapiens
16 BID  
Biochemical Activity Homo sapiens
17 SLK 9748
Invivo Homo sapiens
Invitro Homo sapiens
18 SRP72 6731
Invitro Homo sapiens
19 TGM2 7052
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 TRAF3 7187
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
21 BLM 641
Biochemical Activity Homo sapiens
22 DCTN1 1639
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
23 GORASP1 64689
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
24 RFC1 5981
Invitro Homo sapiens
25 BCL2  
Invivo Homo sapiens
Biochemical Activity Homo sapiens
26 SREBF2 6721
Biochemical Activity Homo sapiens
27 KRT8 3856
Affinity Capture-MS Homo sapiens
28 USO1 8615
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
29 CASP3 836
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
30 MCL1 4170
Biochemical Activity Homo sapiens
31 DCC  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
32 NFE2L2 4780
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
33 BIRC7  
Reconstituted Complex Homo sapiens
34 PSEN1 5663
Affinity Capture-MS Homo sapiens
35 HSPD1 3329
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
36 PDE5A 8654
Invitro Homo sapiens
37 BMX  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
38 PICALM 8301
Invitro Homo sapiens
39 DEDD  
Affinity Capture-MS Homo sapiens
40 BIRC5  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
41 HCLS1 3059
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
42 BIRC3 330
Invitro Homo sapiens
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