Gene description for RBX1
Gene name ring-box 1, E3 ubiquitin protein ligase
Gene symbol RBX1
Other names/aliases BA554C12.1
RNF75
ROC1
Species Homo sapiens
 Database cross references - RBX1
ExoCarta ExoCarta_9978
Entrez Gene 9978
HGNC 9928
MIM 603814
UniProt P62877  
 RBX1 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for RBX1
Molecular Function
    ubiquitin-ubiquitin ligase activity GO:0034450 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin protein ligase binding GO:0031625 IDA
    protein complex binding GO:0032403 IEA
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 TAS
    NEDD8 transferase activity GO:0019788 IDA
    ligase activity GO:0016874 IEA
    protein binding GO:0005515 IPI
    cullin family protein binding GO:0097602 IDA
Biological Process
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    protein neddylation GO:0045116 IDA
    protein monoubiquitination GO:0006513 IDA
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    protein ubiquitination GO:0016567 IDA
    cellular response to hypoxia GO:0071456 TAS
    DNA repair GO:0006281 TAS
    Notch signaling pathway GO:0007219 TAS
    DNA damage response, detection of DNA damage GO:0042769 TAS
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 ISS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    viral process GO:0016032 TAS
    translesion synthesis GO:0019985 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
Subcellular Localization
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 IDA
    Cul4B-RING E3 ubiquitin ligase complex GO:0031465 IDA
    Cul7-RING ubiquitin ligase complex GO:0031467 IDA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
    VCB complex GO:0030891 IEA
    cullin-RING ubiquitin ligase complex GO:0031461 IDA
    nuclear SCF ubiquitin ligase complex GO:0043224 IBA
    cytosol GO:0005829 TAS
    Cul2-RING ubiquitin ligase complex GO:0031462 IDA
    SCF ubiquitin ligase complex GO:0019005 ISS
    Cul5-RING ubiquitin ligase complex GO:0031466 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RBX1 in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RBX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CUL7  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 CUL1 8454
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 CCND2  
Affinity Capture-Western Homo sapiens
4 HSPA8 3312
Affinity Capture-MS Homo sapiens
5 FBXO18 84893
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
6 RFWD2  
Affinity Capture-Western Homo sapiens
7 SKP1 6500
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 CUL3 8452
Affinity Capture-MS Homo sapiens
9 FBXO22 26263
Affinity Capture-MS Homo sapiens
10 VHL  
Affinity Capture-MS Homo sapiens
11 CUL4B 8450
Affinity Capture-MS Homo sapiens
12 CAND1 55832
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 TUBA1B 10376
Affinity Capture-MS Homo sapiens
14 FBXO3  
Affinity Capture-MS Homo sapiens
15 NEDD8 4738
Affinity Capture-MS Homo sapiens
16 HSPA1A 3303
Affinity Capture-MS Homo sapiens
17 FBXW8 26259
Affinity Capture-Western Homo sapiens
18 CUL2 8453
Affinity Capture-MS Homo sapiens
19 DET1  
Affinity Capture-Western Homo sapiens
20 CCND1 595
Affinity Capture-Western Homo sapiens
21 CDC34  
Reconstituted Complex Homo sapiens
22 ACTG1 71
Affinity Capture-MS Homo sapiens
23 CUL4A 8451
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CCND3  
Affinity Capture-Western Homo sapiens
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