Gene description for FBXO4
Gene name F-box protein 4
Gene symbol FBXO4
Other names/aliases FBX4
Species Homo sapiens
 Database cross references - FBXO4
ExoCarta ExoCarta_26272
Vesiclepedia VP_26272
Entrez Gene 26272
HGNC 13583
MIM 609090
UniProt Q9UKT5  
 FBXO4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for FBXO4
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IPI
    ubiquitin protein ligase activity GO:0061630 IEA
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    protein polyubiquitination GO:0000209 IDA
    telomere maintenance GO:0000723 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    post-transcriptional regulation of gene expression GO:0010608 IEA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 TAS
    cellular homeostasis GO:0019725 IEA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IDA
    positive regulation of protein ubiquitination GO:0031398 IDA
    regulation of protein stability GO:0031647 IDA
    regulation of protein stability GO:0031647 IMP
    protein destabilization GO:0031648 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 IC
    positive regulation of telomere maintenance via telomerase GO:0032212 IMP
    common myeloid progenitor cell proliferation GO:0035726 IEA
    negative regulation of fibroblast proliferation GO:0048147 IEA
    cellular response to ionizing radiation GO:0071479 IEA
    cellular senescence GO:0090398 IEA
    negative regulation of protein localization to nucleus GO:1900181 IEA
    positive regulation of protein polyubiquitination GO:1902916 IEA
    regulation of DNA damage checkpoint GO:2000001 IEA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    SCF ubiquitin ligase complex GO:0019005 IBA
    SCF ubiquitin ligase complex GO:0019005 IDA
 Experiment description of studies that identified FBXO4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FBXO4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ESPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 FBXO4 26272
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
3 CDC34 997
Biochemical Activity Homo sapiens
4 CUL1 8454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RNF40 9810
Co-fractionation Homo sapiens
6 TNNI3K  
Two-hybrid Homo sapiens
7 TP53 7157
Affinity Capture-Western Homo sapiens
8 YWHAE 7531
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 MCL1 4170
Affinity Capture-Western Homo sapiens
10 SKP1 6500
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 AP1M1 8907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RBX1 9978
Affinity Capture-MS Homo sapiens
13 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
14 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
15 DEFA1B 728358
Affinity Capture-MS Homo sapiens
16 PCBP3 54039
Affinity Capture-MS Homo sapiens
17 UBE2D2 7322
Biochemical Activity Homo sapiens
18 TERF1 7013
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
19 CDK4 1019
Affinity Capture-Western Homo sapiens
20 AP1B1 162
Affinity Capture-MS Homo sapiens
21 FBXW8 26259
Affinity Capture-Western Homo sapiens
22 CCND1 595
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
23 NEDD8 4738
Affinity Capture-MS Homo sapiens
24 AMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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