Gene description for DEFA1B
Gene name defensin, alpha 1B
Gene symbol DEFA1B
Other names/aliases HNP-1
HP-1
HP1
Species Homo sapiens
 Database cross references - DEFA1B
ExoCarta ExoCarta_728358
Entrez Gene 728358
HGNC 33596
UniProt P59665  
 DEFA1B identified in exosomes derived from the following tissue/cell type
Saliva 19199708    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for DEFA1B
Biological Process
    innate immune response GO:0045087 TAS
    antibacterial humoral response GO:0019731 IDA
    defense response to Gram-positive bacterium GO:0050830 IDA
    defense response to virus GO:0051607 IEA
    killing of cells of other organism GO:0031640 IEA
    intracellular estrogen receptor signaling pathway GO:0030520 IDA
    innate immune response in mucosa GO:0002227 IDA
    defense response to fungus GO:0050832 IEA
    immune response GO:0006955 TAS
    chemotaxis GO:0006935 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    Golgi lumen GO:0005796 TAS
    azurophil granule lumen GO:0035578 TAS
    extracellular region GO:0005576 TAS
    extracellular matrix GO:0031012 IDA
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified DEFA1B in exosomes
1
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DEFA1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DEFA1B is involved
PathwayEvidenceSource
Alpha-defensins TAS Reactome
Defensins TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here