Gene description for UBQLN2
Gene name ubiquilin 2
Gene symbol UBQLN2
Other names/aliases ALS15
CHAP1
DSK2
HRIHFB2157
N4BP4
PLIC2
Species Homo sapiens
 Database cross references - UBQLN2
ExoCarta ExoCarta_29978
Vesiclepedia VP_29978
Entrez Gene 29978
HGNC 12509
MIM 300264
UniProt Q9UHD9  
 UBQLN2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for UBQLN2
Molecular Function
    protein binding GO:0005515 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    identical protein binding GO:0042802 IPI
    molecular condensate scaffold activity GO:0140693 IDA
Biological Process
    autophagosome assembly GO:0000045 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    regulation of macroautophagy GO:0016241 IBA
    regulation of macroautophagy GO:0016241 IMP
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 IMP
    negative regulation of clathrin-dependent endocytosis GO:1900186 IMP
    negative regulation of G protein-coupled receptor internalization GO:1904021 IMP
    positive regulation of ERAD pathway GO:1904294 IMP
    regulation of autophagosome assembly GO:2000785 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    autophagosome GO:0005776 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified UBQLN2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBQLN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-Western Homo sapiens
2 MINOS1-NBL1  
Two-hybrid Homo sapiens
3 P4HB 5034
Co-fractionation Homo sapiens
4 CTAG1A  
Two-hybrid Homo sapiens
5 BPIFA1 51297
Two-hybrid Homo sapiens
6 ZG16  
Two-hybrid Homo sapiens
7 RNASEH2B  
Co-fractionation Homo sapiens
8 NPLOC4 55666
Co-fractionation Homo sapiens
9 UBE2A  
Co-fractionation Homo sapiens
10 RPS28 6234
Cross-Linking-MS (XL-MS) Homo sapiens
11 CCL3  
Two-hybrid Homo sapiens
12 SNAP23 8773
Co-fractionation Homo sapiens
13 PTGDS  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 ZFAND2B  
Two-hybrid Homo sapiens
15 TPD52L2 7165
Co-fractionation Homo sapiens
16 KRT6A 3853
Two-hybrid Homo sapiens
17 UFSP1  
Two-hybrid Homo sapiens
18 RPA2 6118
Co-fractionation Homo sapiens
19 ADSL 158
Co-fractionation Homo sapiens
20 C2orf40  
Two-hybrid Homo sapiens
21 FBXO25  
Biochemical Activity Homo sapiens
22 CTDNEP1 23399
Two-hybrid Homo sapiens
23 CLPSL2  
Two-hybrid Homo sapiens
24 CMPK1 51727
Co-fractionation Homo sapiens
25 HSPA13 6782
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 NUPL1 9818
Two-hybrid Homo sapiens
27 UBE3A 7337
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
28 USP9X 8239
Co-fractionation Homo sapiens
29 UBC 7316
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
30 C4orf26  
Two-hybrid Homo sapiens
31 PSMD14 10213
Affinity Capture-MS Homo sapiens
32 NPBWR1  
Two-hybrid Homo sapiens
33 TXNDC12 51060
Two-hybrid Homo sapiens
34 TRIM32 22954
Two-hybrid Homo sapiens
35 PRPF40A 55660
Two-hybrid Homo sapiens
36 DEFA1B 728358
Two-hybrid Homo sapiens
37 AZGP1 563
Two-hybrid Homo sapiens
38 HSD17B12 51144
Two-hybrid Homo sapiens
39 TMEM123 114908
Two-hybrid Homo sapiens
40 SLPI 6590
Two-hybrid Homo sapiens
41 ETNK1  
Two-hybrid Homo sapiens
42 ANKRD33  
Two-hybrid Homo sapiens
43 COL10A1  
Two-hybrid Homo sapiens
44 RNH1 6050
Co-fractionation Homo sapiens
45 AGR3 155465
Two-hybrid Homo sapiens
46 SLC29A2 3177
Two-hybrid Homo sapiens
47 MIEF2  
Two-hybrid Homo sapiens
48 LAIR2  
Two-hybrid Homo sapiens
49 OR6T1  
Affinity Capture-MS Homo sapiens
50 CARKD 55739
Two-hybrid Homo sapiens
51 PLAUR 5329
Co-fractionation Homo sapiens
52 FAM57A  
Affinity Capture-MS Homo sapiens
53 SMR3B  
Two-hybrid Homo sapiens
54 PODXL 5420
Two-hybrid Homo sapiens
55 COL8A1 1295
Two-hybrid Homo sapiens
56 C7orf76  
Affinity Capture-MS Homo sapiens
57 C1QTNF4  
Two-hybrid Homo sapiens
58 GPR162  
Two-hybrid Homo sapiens
59 NPVF  
Two-hybrid Homo sapiens
60 FGF17  
Two-hybrid Homo sapiens
61 NT5C3A 51251
Two-hybrid Homo sapiens
62 PSMD12 5718
Affinity Capture-MS Homo sapiens
63 DNAJB1 3337
Co-fractionation Homo sapiens
64 C1QC 714
Two-hybrid Homo sapiens
65 YAP1 10413
Co-fractionation Homo sapiens
66 EFEMP1 2202
Two-hybrid Homo sapiens
67 DYNLL1 8655
Affinity Capture-MS Homo sapiens
68 AARSD1 80755
Co-fractionation Homo sapiens
69 FAM127C  
Two-hybrid Homo sapiens
70 CYR61 3491
Two-hybrid Homo sapiens
71 SRP68 6730
Two-hybrid Homo sapiens
72 MYO15B 80022
Two-hybrid Homo sapiens
73 F10 2159
Two-hybrid Homo sapiens
74 KRTAP19-5  
Two-hybrid Homo sapiens
75 NOL3 8996
Two-hybrid Homo sapiens
76 CCK  
Two-hybrid Homo sapiens
77 C6orf15  
Two-hybrid Homo sapiens
78 CST1  
Two-hybrid Homo sapiens
79 SEC23A 10484
Co-fractionation Homo sapiens
80 PSMD6 9861
Affinity Capture-MS Homo sapiens
81 PSMA1 5682
Co-fractionation Homo sapiens
82 HRASLS2  
Two-hybrid Homo sapiens
83 FAF2 23197
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
84 LY6G6D  
Two-hybrid Homo sapiens
85 UBAC1 10422
Two-hybrid Homo sapiens
86 MARCKS 4082
Co-fractionation Homo sapiens
87 LMO4  
Two-hybrid Homo sapiens
88 RAD23A 5886
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
89 SMIM2  
Two-hybrid Homo sapiens
90 RNF11 26994
Two-hybrid Homo sapiens
91 BAG6 7917
Two-hybrid Homo sapiens
92 RNF185  
Affinity Capture-MS Homo sapiens
93 EMC8 10328
Affinity Capture-MS Homo sapiens
94 INSIG2  
Affinity Capture-MS Homo sapiens
95 UBE2V1 7335
Two-hybrid Homo sapiens
96 VTN 7448
Two-hybrid Homo sapiens
97 APOC2 344
Two-hybrid Homo sapiens
98 PMEPA1 56937
Two-hybrid Homo sapiens
99 KRTAP19-3  
Two-hybrid Homo sapiens
100 STAM2 10254
Two-hybrid Homo sapiens
101 PRAP1  
Two-hybrid Homo sapiens
102 PSMC2 5701
Reconstituted Complex Homo sapiens
103 RAD23B 5887
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
104 PPIB 5479
Two-hybrid Homo sapiens
105 UBQLN1 29979
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
106 Cxx1a  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
107 ITPRIPL1  
Two-hybrid Homo sapiens
108 PPIH 10465
Two-hybrid Homo sapiens
109 BCL2L11  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
110 PSMD4 5710
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
111 ATP6V1G1 9550
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
112 NOC4L 79050
Two-hybrid Homo sapiens
113 CNNM3 26505
Two-hybrid Homo sapiens
114 GHRL  
Two-hybrid Homo sapiens
115 KRTAP12-1  
Two-hybrid Homo sapiens
116 CSTF2 1478
Two-hybrid Homo sapiens
117 OR4N2  
Affinity Capture-MS Homo sapiens
118 DEFB115  
Two-hybrid Homo sapiens
119 PSMC1 5700
Reconstituted Complex Homo sapiens
120 UBE2I 7329
Two-hybrid Homo sapiens
121 KRTAP13-1  
Two-hybrid Homo sapiens
122 SPAG11B  
Two-hybrid Homo sapiens
123 HTT 3064
Affinity Capture-Western Homo sapiens
124 UBXN7 26043
Two-hybrid Homo sapiens
125 ASCL1  
Two-hybrid Homo sapiens
126 PLEKHB2 55041
Two-hybrid Homo sapiens
127 GALP  
Two-hybrid Homo sapiens
128 RSRC2  
Two-hybrid Homo sapiens
129 SMIM11  
Two-hybrid Homo sapiens
130 TXNDC5 81567
Two-hybrid Homo sapiens
131 PFDN2 5202
Co-fractionation Homo sapiens
132 SLC16A3 9123
Two-hybrid Homo sapiens
133 IGLL1  
Two-hybrid Homo sapiens
134 HERC6  
Affinity Capture-Western Homo sapiens
135 PNMA3  
Two-hybrid Homo sapiens
136 RNF128  
Two-hybrid Homo sapiens
137 SMIM19  
Two-hybrid Homo sapiens
138 NTRK1 4914
Affinity Capture-MS Homo sapiens
139 SCG5  
Two-hybrid Homo sapiens
140 NPY  
Two-hybrid Homo sapiens
141 SLITRK1 114798
Two-hybrid Homo sapiens
142 CCL7 6354
Two-hybrid Homo sapiens
143 EGFL6 25975
Two-hybrid Homo sapiens
144 PRR4 11272
Two-hybrid Homo sapiens
145 AGR2 10551
Two-hybrid Homo sapiens
146 NME3 4832
Two-hybrid Homo sapiens
147 C12orf10 60314
Co-fractionation Homo sapiens
148 HEMK1  
Two-hybrid Homo sapiens
149 SUSD4  
Two-hybrid Homo sapiens
150 OSMR 9180
Two-hybrid Homo sapiens
151 EPN1 29924
Affinity Capture-Western Homo sapiens
152 NRN1L  
Two-hybrid Homo sapiens
153 SPN 6693
Two-hybrid Homo sapiens
154 FAM127A  
Two-hybrid Homo sapiens
155 VENTX  
Two-hybrid Homo sapiens
156 PSMD2 5708
Two-hybrid Homo sapiens
157 HNRNPA3 220988
Two-hybrid Homo sapiens
158 SCGB2B2  
Two-hybrid Homo sapiens
159 TOMM20L  
Two-hybrid Homo sapiens
160 NDOR1  
Two-hybrid Homo sapiens
161 CD93  
Two-hybrid Homo sapiens
162 HERPUD1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
163 STAM 8027
Co-fractionation Homo sapiens
164 SUOX  
Two-hybrid Homo sapiens
165 IGBP1 3476
Co-fractionation Homo sapiens
166 FAM127B  
Two-hybrid Homo sapiens
167 PARM1  
Two-hybrid Homo sapiens
168 GUCA2A  
Two-hybrid Homo sapiens
169 C1QTNF2  
Two-hybrid Homo sapiens
170 SLC17A2  
Affinity Capture-MS Homo sapiens
171 PDE12 201626
Co-fractionation Homo sapiens
172 OR7D4  
Two-hybrid Homo sapiens
173 EPS15 2060
Affinity Capture-Western Homo sapiens
174 EMC3 55831
Affinity Capture-MS Homo sapiens
175 DEFA1 1667
Two-hybrid Homo sapiens
176 CAPN15  
Two-hybrid Homo sapiens
177 DMKN  
Two-hybrid Homo sapiens
178 PINK1  
Affinity Capture-MS Homo sapiens
179 CSN2 1447
Two-hybrid Homo sapiens
180 RNF208  
Two-hybrid Homo sapiens
181 MTX2 10651
Two-hybrid Homo sapiens
182 SNRPB 6628
Two-hybrid Homo sapiens
183 C1QA 712
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
184 UBQLN2 29978
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
185 NPPA  
Two-hybrid Homo sapiens
186 SOD3 6649
Two-hybrid Homo sapiens
187 TFF3  
Two-hybrid Homo sapiens
188 MBL2 4153
Two-hybrid Homo sapiens
189 QPCT 25797
Two-hybrid Homo sapiens
190 LOC102723553  
Two-hybrid Homo sapiens
191 FAM46B  
Two-hybrid Homo sapiens
192 CDH15 1013
Two-hybrid Homo sapiens
193 PSMA2 5683
Reconstituted Complex Homo sapiens
194 POM121  
Two-hybrid Homo sapiens
195 FUCA1 2517
Two-hybrid Homo sapiens
196 SERPINE1 5054
Two-hybrid Homo sapiens
197 TNFRSF18  
Two-hybrid Homo sapiens
198 KISS1  
Two-hybrid Homo sapiens
199 HNRNPA1 3178
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
200 RBMS1 5937
Co-fractionation Homo sapiens
201 CSTF2T  
Two-hybrid Homo sapiens
202 EEF1B2 1933
Co-fractionation Homo sapiens
203 UACA 55075
Two-hybrid Homo sapiens
204 ZFYVE19 84936
Co-fractionation Homo sapiens
205 VBP1 7411
Co-fractionation Homo sapiens
206 SEMG1 6406
Two-hybrid Homo sapiens
207 PRKACA 5566
Co-fractionation Homo sapiens