Gene description for WDR4
Gene name WD repeat domain 4
Gene symbol WDR4
Other names/aliases TRM82
TRMT82
Species Homo sapiens
 Database cross references - WDR4
ExoCarta ExoCarta_10785
Entrez Gene 10785
HGNC 12756
MIM 605924
UniProt P57081  
 WDR4 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for WDR4
Molecular Function
    tRNA (guanine-N7-)-methyltransferase activity GO:0008176 IDA
    protein binding GO:0005515 IPI
Biological Process
    tRNA methylation GO:0030488 IEA
    tRNA modification GO:0006400 IDA
    RNA (guanine-N7)-methylation GO:0036265 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified WDR4 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDR4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 METTL1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which WDR4 is involved
No pathways found





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