Gene description for TP53RK
Gene name TP53 regulating kinase
Gene symbol TP53RK
Other names/aliases BUD32
C20orf64
Nori-2
Nori-2p
PRPK
dJ101A2
Species Homo sapiens
 Database cross references - TP53RK
ExoCarta ExoCarta_112858
Entrez Gene 112858
HGNC 16197
MIM 608679
UniProt Q96S44  
 TP53RK identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for TP53RK
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    p53 binding GO:0002039 IDA
    protein binding GO:0005515 IPI
    hydrolase activity GO:0016787 IEA
    ATP binding GO:0005524 IEA
Biological Process
    tRNA processing GO:0008033 IEA
    protein phosphorylation GO:0006468 IDA
    threonylcarbamoyladenosine metabolic process GO:0070525 IBA
Subcellular Localization
    membrane GO:0016020 IEA
    EKC/KEOPS complex GO:0000408 IBA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IBA
 Experiment description of studies that identified TP53RK in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TP53RK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRKB  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 TP53  
Reconstituted Complex Homo sapiens
3 CALCOCO2  
Two-hybrid Homo sapiens
4 CGI-121  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which TP53RK is involved
No pathways found





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