Gene description for ANO4
Gene name anoctamin 4
Gene symbol ANO4
Other names/aliases TMEM16D
Species Homo sapiens
 Database cross references - ANO4
ExoCarta ExoCarta_121601
Vesiclepedia VP_121601
Entrez Gene 121601
HGNC 23837
MIM 610111
UniProt Q32M45  
 ANO4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ANO4
Molecular Function
    intracellularly calcium-gated chloride channel activity GO:0005229 IBA
    intracellularly calcium-gated chloride channel activity GO:0005229 IDA
    chloride channel activity GO:0005254 TAS
    phospholipid scramblase activity GO:0017128 IEA
    protein dimerization activity GO:0046983 IEA
Biological Process
    monoatomic ion transmembrane transport GO:0034220 TAS
    establishment of localization in cell GO:0051649 IEA
    calcium activated phosphatidylserine scrambling GO:0061589 IEA
    calcium activated phosphatidylcholine scrambling GO:0061590 IEA
    calcium activated galactosylceramide scrambling GO:0061591 IEA
    chloride transmembrane transport GO:1902476 IBA
    chloride transmembrane transport GO:1902476 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified ANO4 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ANO4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MIA3 375056
Affinity Capture-MS Homo sapiens
2 ENOSF1  
Affinity Capture-MS Homo sapiens
3 TP53RK 112858
Affinity Capture-MS Homo sapiens
4 PREB 10113
Affinity Capture-MS Homo sapiens
5 TPRKB 51002
Affinity Capture-MS Homo sapiens
6 PPP1R15B  
Affinity Capture-MS Homo sapiens
7 TPGS1  
Affinity Capture-MS Homo sapiens
8 ANO6 196527
Affinity Capture-MS Homo sapiens
9 DNAJC18  
Affinity Capture-MS Homo sapiens
10 DNAJC30  
Affinity Capture-MS Homo sapiens
11 ANO9  
Affinity Capture-MS Homo sapiens
12 CTAGE5 4253
Affinity Capture-MS Homo sapiens
13 OSGEP 55644
Affinity Capture-MS Homo sapiens
14 FBXL19  
Affinity Capture-MS Homo sapiens
15 C2orf49 79074
Affinity Capture-MS Homo sapiens
16 PWP2 5822
Affinity Capture-MS Homo sapiens
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