Gene description for ANO4
Gene name anoctamin 4
Gene symbol ANO4
Other names/aliases TMEM16D
Species Homo sapiens
 Database cross references - ANO4
ExoCarta ExoCarta_121601
Entrez Gene 121601
HGNC 23837
MIM 610111
UniProt Q32M45  
 ANO4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ANO4
Molecular Function
    phospholipid scramblase activity GO:0017128 IEA
    intracellular calcium activated chloride channel activity GO:0005229 IDA
Biological Process
    calcium activated phosphatidylserine scrambling GO:0061589 IEA
    calcium activated galactosylceramide scrambling GO:0061591 IEA
    lipid transport GO:0006869 IEA
    transmembrane transport GO:0055085 TAS
    chloride transport GO:0006821 IDA
    calcium activated phosphatidylcholine scrambling GO:0061590 IEA
    ion transmembrane transport GO:0034220 TAS
    chloride transmembrane transport GO:1902476 IDA
Subcellular Localization
    intracellular GO:0005622 ISS
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified ANO4 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ANO4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ANO4 is involved
PathwayEvidenceSource
Stimuli-sensing channels TAS Reactome





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