Gene description for PWP2
Gene name PWP2 periodic tryptophan protein homolog (yeast)
Gene symbol PWP2
Other names/aliases EHOC-17
PWP2H
UTP1
Species Homo sapiens
 Database cross references - PWP2
ExoCarta ExoCarta_5822
Vesiclepedia VP_5822
Entrez Gene 5822
HGNC 9711
MIM 601475
UniProt Q15269  
 PWP2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PWP2
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    ribosomal small subunit assembly GO:0000028 IBA
    maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000462 IBA
    ribosomal small subunit biogenesis GO:0042274 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 IDA
    Pwp2p-containing subcomplex of 90S preribosome GO:0034388 IBA
 Experiment description of studies that identified PWP2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PWP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UTP18  
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
4 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
5 WDR3  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
6 TARDBP 23435
Affinity Capture-MS Homo sapiens
7 BBS7 55212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 RPL13A 23521
Affinity Capture-MS Homo sapiens
9 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CHCHD1  
Proximity Label-MS Homo sapiens
11 ZNF71  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 FGF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 UNC45B  
Affinity Capture-MS Homo sapiens
14 WDR36 134430
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
15 RPS3 6188
Affinity Capture-MS Homo sapiens
16 DNAJB6 10049
Proximity Label-MS Homo sapiens
17 USP36  
Affinity Capture-MS Homo sapiens
18 C9orf78 51759
Affinity Capture-MS Homo sapiens
19 GPATCH4 54865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KIF23 9493
Affinity Capture-MS Homo sapiens
21 IMP3 55272
Co-fractionation Homo sapiens
22 MPHOSPH10 10199
Co-fractionation Homo sapiens
23 PRC1 9055
Affinity Capture-MS Homo sapiens
24 ANOS1 3730
Affinity Capture-MS Homo sapiens
25 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 POLR1E  
Proximity Label-MS Homo sapiens
27 OBSL1 23363
Affinity Capture-MS Homo sapiens
28 TOM1L1 10040
Two-hybrid Homo sapiens
29 EXOSC4 54512
Affinity Capture-MS Homo sapiens
30 BRD4 23476
Affinity Capture-MS Homo sapiens
31 DDX18 8886
Co-fractionation Homo sapiens
32 KLHL20  
Affinity Capture-MS Homo sapiens
33 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CUL1 8454
Affinity Capture-MS Homo sapiens
35 MPHOSPH6 10200
Affinity Capture-MS Homo sapiens
36 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KIF14 9928
Affinity Capture-MS Homo sapiens
38 CHMP4C 92421
Affinity Capture-MS Homo sapiens
39 KIF20A 10112
Affinity Capture-MS Homo sapiens
40 RPL4 6124
Affinity Capture-MS Homo sapiens
41 RRP9 9136
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
42 ANO4 121601
Affinity Capture-MS Homo sapiens
43 BAG6 7917
Affinity Capture-MS Homo sapiens
44 DTNBP1  
Affinity Capture-MS Homo sapiens
45 MAPT  
Two-hybrid Homo sapiens
46 CHMP4B 128866
Affinity Capture-MS Homo sapiens
47 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 DCAF7 10238
Two-hybrid Homo sapiens
49 IFI16 3428
Affinity Capture-MS Homo sapiens
50 EXOSC5 56915
Affinity Capture-MS Homo sapiens
51 RPL36 25873
Affinity Capture-MS Homo sapiens
52 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
53 SPDYE4  
Proximity Label-MS Homo sapiens
54 HECTD1 25831
Affinity Capture-MS Homo sapiens
55 EED  
Affinity Capture-MS Homo sapiens
56 MAGEB2 4113
Affinity Capture-MS Homo sapiens
57 CD3EAP  
Proximity Label-MS Homo sapiens
58 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 ANLN 54443
Affinity Capture-MS Homo sapiens
60 FGFBP1 9982
Affinity Capture-MS Homo sapiens
61 XPO1 7514
Affinity Capture-MS Homo sapiens
62 ECT2 1894
Affinity Capture-MS Homo sapiens
63 SIRT7  
Affinity Capture-MS Homo sapiens
64 ENOSF1  
Affinity Capture-MS Homo sapiens
65 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 DCAF13 25879
Affinity Capture-MS Homo sapiens
67 EGFR 1956
Negative Genetic Homo sapiens
68 HECW2  
Affinity Capture-MS Homo sapiens
69 ZNF692  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 DNAJC9 23234
Proximity Label-MS Homo sapiens
71 WDR75  
Affinity Capture-MS Homo sapiens
72 TGFA  
Affinity Capture-MS Homo sapiens
73 DDRGK1 65992
Affinity Capture-MS Homo sapiens
74 RNF2  
Affinity Capture-MS Homo sapiens
75 H2AFB2  
Affinity Capture-MS Homo sapiens
76 BRD3 8019
Affinity Capture-MS Homo sapiens
77 RPL15 6138
Affinity Capture-MS Homo sapiens
78 GYPA  
Affinity Capture-MS Homo sapiens
79 SURF6  
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 SIRT6  
Affinity Capture-MS Homo sapiens
82 HDGF 3068
Affinity Capture-MS Homo sapiens
83 TP53 7157
Affinity Capture-MS Homo sapiens
84 ZNF746  
Affinity Capture-MS Homo sapiens
85 BYSL 705
Co-fractionation Homo sapiens
86 Atrx  
Affinity Capture-MS Mus musculus
87 SMURF1 57154
Affinity Capture-MS Homo sapiens
88 TBL3 10607
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
89 UFL1 23376
Affinity Capture-MS Homo sapiens
90 RBM4 5936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 RPS24 6229
Proximity Label-MS Homo sapiens
92 TECTB  
Affinity Capture-MS Homo sapiens
93 RPS6 6194
Proximity Label-MS Homo sapiens
94 GRSF1 2926
Proximity Label-MS Homo sapiens
95 IPO13 9670
Co-fractionation Homo sapiens
96 CUL7 9820
Affinity Capture-MS Homo sapiens
97 KRAS 3845
Negative Genetic Homo sapiens
98 RPA3 6119
Proximity Label-MS Homo sapiens
99 ZNF467  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here