Gene description for DCAF13
Gene name DDB1 and CUL4 associated factor 13
Gene symbol DCAF13
Other names/aliases GM83
HSPC064
WDSOF1
Species Homo sapiens
 Database cross references - DCAF13
ExoCarta ExoCarta_25879
Entrez Gene 25879
HGNC 24535
MIM 616196
UniProt Q9NV06  
 DCAF13 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DCAF13
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000462 IBA
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IDA
    nucleolus GO:0005730 IBA
    small-subunit processome GO:0032040 IBA
 Experiment description of studies that identified DCAF13 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DCAF13
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DCAF13 is involved
No pathways found





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