Gene description for DCAF13
Gene name DDB1 and CUL4 associated factor 13
Gene symbol DCAF13
Other names/aliases GM83
HSPC064
WDSOF1
Species Homo sapiens
 Database cross references - DCAF13
ExoCarta ExoCarta_25879
Vesiclepedia VP_25879
Entrez Gene 25879
HGNC 24535
MIM 616196
UniProt Q9NV06  
 DCAF13 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DCAF13
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    nuclear estrogen receptor binding GO:0030331 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000462 IBA
    oocyte growth GO:0001555 ISS
    rRNA processing GO:0006364 ISS
    spindle assembly involved in female meiosis GO:0007056 IDA
    protein ubiquitination GO:0016567 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    epigenetic programming in the zygotic pronuclei GO:0044725 IDA
    decidualization GO:0046697 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cell junction GO:0030054 IDA
    small-subunit processome GO:0032040 IBA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IDA
 Experiment description of studies that identified DCAF13 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DCAF13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 H2AFJ 55766
Affinity Capture-MS Homo sapiens
3 SENP3 26168
Affinity Capture-MS Homo sapiens
4 RIOK1 83732
Affinity Capture-MS Homo sapiens
5 RSL1D1 26156
Affinity Capture-MS Homo sapiens
6 GNPDA2 132789
Affinity Capture-MS Homo sapiens
7 FAM98A 25940
Affinity Capture-MS Homo sapiens
8 NOP58 51602
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
9 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TK1 7083
Two-hybrid Homo sapiens
11 KIAA0020 9933
Co-fractionation Homo sapiens
12 PPIL4  
Affinity Capture-MS Homo sapiens
13 HIST1H1D 3007
Cross-Linking-MS (XL-MS) Homo sapiens
14 TGS1  
Affinity Capture-MS Homo sapiens
15 NOP56 10528
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
16 TAS2R42  
Affinity Capture-MS Homo sapiens
17 RPS19 6223
Affinity Capture-MS Homo sapiens
18 ATM 472
Affinity Capture-MS Homo sapiens
19 PRPF40A 55660
Affinity Capture-MS Homo sapiens
20 PWP2 5822
Affinity Capture-MS Homo sapiens
21 WDR36 134430
Co-fractionation Homo sapiens
22 RPS3 6188
Affinity Capture-MS Homo sapiens
23 DNAJB6 10049
Proximity Label-MS Homo sapiens
24 USP36  
Affinity Capture-MS Homo sapiens
25 FIP1L1 81608
Affinity Capture-MS Homo sapiens
26 FAM76B  
Affinity Capture-MS Homo sapiens
27 E2F2 1870
Affinity Capture-MS Homo sapiens
28 NDE1 54820
Affinity Capture-MS Homo sapiens
29 BPTF 2186
Affinity Capture-MS Homo sapiens
30 EXOSC2 23404
Affinity Capture-MS Homo sapiens
31 HOXA5  
Affinity Capture-MS Homo sapiens
32 ATXN2L 11273
Affinity Capture-MS Homo sapiens
33 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 EPB41L2 2037
Affinity Capture-MS Homo sapiens
35 COL8A1 1295
Affinity Capture-MS Homo sapiens
36 CPSF7 79869
Affinity Capture-MS Homo sapiens
37 NPM1 4869
Affinity Capture-MS Homo sapiens
38 ZMYND11 10771
Affinity Capture-MS Homo sapiens
39 FBL 2091
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 Set 56086
Affinity Capture-MS Mus musculus
41 PEA15 8682
Affinity Capture-MS Homo sapiens
42 EXOSC4 54512
Affinity Capture-MS Homo sapiens
43 EED  
Affinity Capture-MS Homo sapiens
44 SDHAP2  
Affinity Capture-MS Homo sapiens
45 DDX18 8886
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
46 UTP20 27340
Co-fractionation Homo sapiens
47 DDX6 1656
Affinity Capture-MS Homo sapiens
48 CDCA8 55143
Affinity Capture-MS Homo sapiens
49 MECP2 4204
Affinity Capture-MS Homo sapiens
50 CSNK1D 1453
Affinity Capture-MS Homo sapiens
51 RPS11 6205
Affinity Capture-MS Homo sapiens
52 FBXW5  
Affinity Capture-MS Homo sapiens
53 DPH1  
Affinity Capture-MS Homo sapiens
54 PES1 23481
Affinity Capture-MS Homo sapiens
55 CIAO1 9391
Affinity Capture-MS Homo sapiens
56 CCT2 10576
Affinity Capture-MS Homo sapiens
57 Pten  
Affinity Capture-MS Mus musculus
58 TMEM14B  
Affinity Capture-MS Homo sapiens
59 UTP14A 10813
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
60 DDX21 9188
Affinity Capture-MS Homo sapiens
61 RBX1 9978
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
62 CUL4B 8450
Affinity Capture-MS Homo sapiens
63 MEPCE 56257
Affinity Capture-MS Homo sapiens
64 RRP9 9136
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
65 RBM4B  
Affinity Capture-MS Homo sapiens
66 NONO 4841
Affinity Capture-MS Homo sapiens
67 CCT5 22948
Affinity Capture-MS Homo sapiens
68 IFI27L1  
Affinity Capture-MS Homo sapiens
69 ABCF2 10061
Affinity Capture-MS Homo sapiens
70 RPS7 6201
Affinity Capture-MS Homo sapiens
71 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 GRWD1 83743
Affinity Capture-MS Homo sapiens
73 NSRP1  
Affinity Capture-MS Homo sapiens
74 S100B 6285
Affinity Capture-MS Homo sapiens
75 NOB1  
Affinity Capture-MS Homo sapiens
76 HECTD1 25831
Affinity Capture-MS Homo sapiens
77 PAXIP1  
Affinity Capture-MS Homo sapiens
78 RRP1B 23076
Affinity Capture-MS Homo sapiens
79 DDX27 55661
Co-fractionation Homo sapiens
80 GTPBP4 23560
Affinity Capture-MS Homo sapiens
81 PIPSL 266971
Affinity Capture-MS Homo sapiens
82 CCNT1  
Affinity Capture-MS Homo sapiens
83 RPS8 6202
Affinity Capture-MS Homo sapiens
84 C1orf189  
Affinity Capture-MS Homo sapiens
85 UTP15 84135
Affinity Capture-MS Homo sapiens
86 TPX2  
Affinity Capture-MS Homo sapiens
87 NUMA1 4926
Cross-Linking-MS (XL-MS) Homo sapiens
88 SGSM2  
Affinity Capture-MS Homo sapiens
89 IPO5 3843
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
90 ABT1 29777
Affinity Capture-MS Homo sapiens
91 JPH3 57338
Affinity Capture-MS Homo sapiens
92 POP1 10940
Affinity Capture-MS Homo sapiens
93 PSENEN  
Affinity Capture-MS Homo sapiens
94 SIRT7  
Affinity Capture-MS Homo sapiens
95 TSPYL5  
Affinity Capture-MS Homo sapiens
96 NOC4L 79050
Affinity Capture-MS Homo sapiens
97 NAT10 55226
Affinity Capture-MS Homo sapiens
98 Emc6  
Affinity Capture-MS Mus musculus
99 DDB2  
Affinity Capture-MS Homo sapiens
100 MRPL9 65005
Affinity Capture-MS Homo sapiens
101 PTEN 5728
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
102 CTTN 2017
Affinity Capture-MS Homo sapiens
103 EGFR 1956
Negative Genetic Homo sapiens
104 BEX1  
Affinity Capture-MS Homo sapiens
105 YY1 7528
Affinity Capture-MS Homo sapiens
106 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 SART1 9092
Affinity Capture-MS Homo sapiens
108 MTF1  
Affinity Capture-MS Homo sapiens
109 JUN 3725
Affinity Capture-MS Homo sapiens
110 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
111 LBH  
Affinity Capture-MS Homo sapiens
112 FTSJ1 24140
Affinity Capture-MS Homo sapiens
113 STAT1 6772
Affinity Capture-MS Homo sapiens
114 PSPC1 55269
Affinity Capture-MS Homo sapiens
115 DDX47 51202
Co-fractionation Homo sapiens
116 PERP 64065
Affinity Capture-Western Homo sapiens
117 EXOSC3 51010
Affinity Capture-MS Homo sapiens
118 TOP1 7150
Affinity Capture-MS Homo sapiens
119 NHP2L1 4809
Affinity Capture-MS Homo sapiens
120 NDC80 10403
Affinity Capture-MS Homo sapiens
121 PPP1R10  
Affinity Capture-MS Homo sapiens
122 DDB1 1642
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
123 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
124 BMI1  
Affinity Capture-MS Homo sapiens
125 RPS29 6235
Affinity Capture-MS Homo sapiens
126 TMSB4Y  
Affinity Capture-MS Homo sapiens
127 FTSJ3 117246
Affinity Capture-MS Homo sapiens
128 GNL3 26354
Affinity Capture-MS Homo sapiens
129 RPS16 6217
Affinity Capture-MS Homo sapiens
130 CBX4  
Affinity Capture-MS Homo sapiens
131 FTL 2512
Affinity Capture-MS Homo sapiens
132 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
133 PDCL3 79031
Affinity Capture-MS Homo sapiens
134 Cenph  
Affinity Capture-MS Mus musculus
135 CPSF6 11052
Affinity Capture-MS Homo sapiens
136 CIT 11113
Affinity Capture-MS Homo sapiens
137 RBM25 58517
Affinity Capture-MS Homo sapiens
138 DNAJA2 10294
Affinity Capture-MS Homo sapiens
139 MCM5 4174
Affinity Capture-MS Homo sapiens
140 POLR1C 9533
Co-fractionation Homo sapiens
141 EHHADH 1962
Affinity Capture-MS Homo sapiens
142 NUFIP2 57532
Affinity Capture-MS Homo sapiens
143 MEN1 4221
Affinity Capture-MS Homo sapiens
144 SQSTM1 8878
Proximity Label-MS Homo sapiens
145 RPS15 6209
Affinity Capture-MS Homo sapiens
146 C1orf35  
Affinity Capture-MS Homo sapiens
147 AP1S2 8905
Affinity Capture-MS Homo sapiens
148 TBL3 10607
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
149 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
150 TAF2  
Affinity Capture-MS Homo sapiens
151 WDR81 124997
Affinity Capture-MS Homo sapiens
152 EZH1  
Affinity Capture-MS Homo sapiens
153 MKI67  
Affinity Capture-MS Homo sapiens
154 NVL  
Affinity Capture-MS Homo sapiens
155 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 ESR1  
Affinity Capture-Western Homo sapiens
157 C20orf197  
Affinity Capture-MS Homo sapiens
158 KRR1 11103
Affinity Capture-MS Homo sapiens
159 SNIP1  
Affinity Capture-MS Homo sapiens
160 TCF4  
Affinity Capture-MS Homo sapiens
161 TMEM257  
Affinity Capture-MS Homo sapiens
162 SPCS1 28972
Affinity Capture-MS Homo sapiens
163 CDK9 1025
Affinity Capture-MS Homo sapiens
164 RPS6 6194
Proximity Label-MS Homo sapiens
165 RPL8 6132
Affinity Capture-MS Homo sapiens
166 DDX42 11325
Affinity Capture-MS Homo sapiens
167 EXOSC8  
Affinity Capture-MS Homo sapiens
168 RPL23A 6147
Affinity Capture-MS Homo sapiens
169 KRAS 3845
Negative Genetic Homo sapiens
170 POLR1D 51082
Co-fractionation Homo sapiens
171 RPA1 6117
Affinity Capture-MS Homo sapiens
172 METTL17  
Affinity Capture-MS Homo sapiens
173 WARS 7453
Two-hybrid Homo sapiens
174 RPA3 6119
Proximity Label-MS Homo sapiens
175 UQCR11  
Affinity Capture-MS Homo sapiens
176 DDX56  
Affinity Capture-MS Homo sapiens
177 DCAF5  
Affinity Capture-MS Homo sapiens
178 NEDD8 4738
Affinity Capture-MS Homo sapiens
179 ZNF677  
Affinity Capture-MS Homo sapiens
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