Gene description for MCM5
Gene name minichromosome maintenance complex component 5
Gene symbol MCM5
Other names/aliases CDC46
P1-CDC46
Species Homo sapiens
 Database cross references - MCM5
ExoCarta ExoCarta_4174
Entrez Gene 4174
HGNC 6948
MIM 602696
UniProt P33992  
 MCM5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MCM5
Molecular Function
    ATP binding GO:0005524 IEA
    chromatin binding GO:0003682 IEA
    DNA helicase activity GO:0003678 IEA
    protein binding GO:0005515 IPI
    DNA replication origin binding GO:0003688 IEA
Biological Process
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    DNA duplex unwinding GO:0032508 IEA
    mitotic cell cycle GO:0000278 TAS
    DNA replication initiation GO:0006270 IEA
    DNA replication GO:0006260 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
Subcellular Localization
    MCM complex GO:0042555 IDA
    membrane GO:0016020 IDA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified MCM5 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCM5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPA2 6118
Two-hybrid Homo sapiens
2 MCM7 4176
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
3 MCM3 4172
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
4 ORC1L  
Affinity Capture-MS Homo sapiens
5 MDFI  
Two-hybrid Homo sapiens
6 CDC7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 STAT1 6772
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 ORC6L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 ORC2L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 MCM2 4171
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 CIAO1 9391
Affinity Capture-MS Homo sapiens
12 CDC5L 988
Affinity Capture-MS Homo sapiens
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